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Association between rs619586 (A/G) polymorphism in the gene encoding lncRNA-MALAT1 with type 2 diabetes susceptibility among the Isfahan population in Iran

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International Journal of Diabetes in Developing Countries Aims and scope Submit manuscript

Abstract

Background and aim

Type 2 diabetes mellitus (T2DM) is a global human disease that affects millions of people. Long non-coding RNAs (LncRNAs) are transcripts with more than two-hundred nucleotides that play essential roles in the management of mRNAs. In the present study, we examined whether the rs619586 (A/G) polymorphism in the gene encoding lncRNA-MALAT1 is associated with the susceptibility to T2DM among the Isfahan population, Iran.

Methods

To this end, a case-control study was conducted on 200 healthy persons and 200 patients with T2DM. The genomic DNA was extracted from blood samples to strengthen the intended fragments containing rs619586 SNP. Using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP), the wild allele (A) and the mutant allele (G) were examined.

Result and conclusion

Results indicated that the mutant allele (G) and mutant genotypes (AG/GG) were absent in T2DM patients. This absence suggests that the rs619586 (A/G) polymorphism in the gene encoding lncRNA-MALAT1 might not be associated with the susceptibility to T2DM among the Isfahan population.

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Acknowledgment

We are thankful for supporting this research from Shahrekord, Isfahan University of Medical Sciences, and Mashhad University of Medical Sciences.

Funding

The study has been financially supported by Islamic Azad University of Shahrekord, Shahrekord, Iran (Grant number; IR.IAU.SHK.REC.1398.022).

Author information

Authors and Affiliations

  1. Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

    Abbas Samadi-Khouzani, Fatemeh Ghafari & Maryam Peymani

  2. Cellular, Molecular and Genetics Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

    Payam Kheirmand Parizi

  3. Medical Genetics Research Center of Genome, Isfahan University of Medical Sciences, Isfahan, Iran

    Payam Kheirmand Parizi

  4. Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Seyed-Alireza Esmaeili

  5. Immunology Department, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Seyed-Alireza Esmaeili

  6. Department of Medical Biotechnology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran

    Amir Abbas Momtazi-Borojeni

  7. Iran’s National Elites Foundation, Tehran, Iran

    Amir Abbas Momtazi-Borojeni

  8. Department of Medical Biotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Amir Abbas Momtazi-Borojeni

Authors
  1. Abbas Samadi-Khouzani
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  2. Payam Kheirmand Parizi
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  3. Fatemeh Ghafari
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  4. Seyed-Alireza Esmaeili
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  5. Maryam Peymani
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  6. Amir Abbas Momtazi-Borojeni
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Corresponding author

Correspondence to Maryam Peymani.

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Conflict of interests

The samples were collected from subjects using EDTA-coated tubes, and the informed agreement was taken from the patients and control participants.

The survey was confirmed by the ethical council of the Islamic Azad University, Shahrekord Branch, Shahrekord, Iran (Ethical code number: IR.IAU.SHK.REC.1398.022).

Conflict of interests

The authors declare no competing interests.

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Samadi-Khouzani, A., Parizi, P.K., Ghafari, F. et al. Association between rs619586 (A/G) polymorphism in the gene encoding lncRNA-MALAT1 with type 2 diabetes susceptibility among the Isfahan population in Iran. Int J Diabetes Dev Ctries 42, 77–81 (2022). https://doi.org/10.1007/s13410-021-00949-1

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  • DOI: https://doi.org/10.1007/s13410-021-00949-1

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