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Differentiation of IMR32 Neuroblastoma Is Accompanied by a Global Change in the Transcriptome

  • BIOCHEMISTRY, BIOPHYSICS, AND MOLECULAR BIOLOGY
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Abstract

Neuroblastoma is one of the most common cancers in infants and is often multidrug-resistant. One of the methods of treating neuroblastomas is to create conditions for their differentiation. In this work, we performed a full-transcriptome analysis of gene expression in an undifferentiated and differentiated in vitro human neuroblastoma cell line IMR-32 and identified the signaling pathways and biological processes that undergo the greatest changes during differentiation. The results obtained show that a complex heterogeneous population of nerve cells is formed at different stages of differentiation. In the cell population of differentiating neuroblastoma, the expression of genes in which cortical neuronal progenitor cells are enriched increases; at the same time, there are cells expressing markers of early postmitotic neurons. Cells differentiate in several different directions according to the type of synaptic mediator. At the same time, the differentiation of IMR-32 cells is accompanied by an increase in the transcription of genes that suppress the differentiation of nerve cells, Sox2 and PROM1, the expression of which is normally suppressed during in vivo differentiation.

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Funding

This work was supported by the Russian Foundation for Basic Research (project no. 18-04-01306) and the Russian Science Foundation (project no. 19-14-00365).

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Authors and Affiliations

  1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia

    A. G. Stepchenko, T. N. Portseva,  Yu. V. Ilyin,  S. G. Georgieva & E. V. Pankratova

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  1. A. G. Stepchenko
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  2. T. N. Portseva
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  3. Yu. V. Ilyin
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  4. S. G. Georgieva
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  5. E. V. Pankratova
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Corresponding author

Correspondence to E. V. Pankratova.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by M. Batrukova

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Stepchenko, A.G., Portseva, T.N., Ilyin, Y.V. et al. Differentiation of IMR32 Neuroblastoma Is Accompanied by a Global Change in the Transcriptome. Dokl Biochem Biophys 497, 90–94 (2021). https://doi.org/10.1134/S1607672921020149

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  • DOI: https://doi.org/10.1134/S1607672921020149

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