Skip to main content
SpringerLink
Log in

Human CTCF Interacts with Drosophila CP190, but not with Kaiso

  • BIOCHEMISTRY, BIOPHYSICS, AND MOLECULAR BIOLOGY
  • Published:
Doklady Biochemistry and Biophysics Aims and scope Submit manuscript

Abstract

Human CTCF (hCTCF) is a major architectural protein in mammals. In Drosophila, the CTCF homologue (dCTCF) interacts with the BTB domain of the CP190 protein, which is involved in the establishment of open chromatin and activity of insulators. Previously, it was shown that the BTB protein Kaiso interacts with hCTCF and regulates its activity. We have carried out a detailed study of the interaction between these proteins in the yeast two-hybrid assay. Surprisingly, Kaiso did not interact with hCTCF and its Drosophila homologue. On the other hand, CP190 interacted with the C-terminus of hCTCF. The results obtained demonstrate that the interaction between CTCF and CP190 proteins is highly conserved. It is likely that humans have other BTB proteins that perform the functions described for the Drosophila CP190.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.

Similar content being viewed by others

REFERENCES

  1. Fedotova, A.A., Bonchuk, A.N., Mogila, V.A., et al., Acta Naturae, 2017, vol. 9, pp. 47–58.

    Article  CAS  Google Scholar 

  2. Arzate-Mejia, R.G., Recillas-Targa, F., and Corces, V.G., Development, 2018, vol. 145, p. 6.

    Article  Google Scholar 

  3. Kyrchanova, O., Ivlieva, T., Toshchakov, S., et al., Nucleic Acids Res., 2011, vol. 39, pp. 3042–3052.

    Article  CAS  Google Scholar 

  4. Bonchuk, A., Maksimenko, O., Kyrchanova, O., et al., BMC Biol., 2015, vol. 13, p. 63.

    Article  Google Scholar 

  5. Szabo, Q., Bantignies, F., Cavalli, G., et al., Sci. Adv., 2019, vol. 5, p. 4.

    Article  Google Scholar 

  6. Bonchuk, A., Kamalyan, S., Mariasina, S., et al., Sci. Rep., 2020, vol. 10, p. 1.

    Article  Google Scholar 

  7. Defossez, P.A., Kelly, K.F., Filion, G.J., et al., J. Biol. Chem., 2005, vol. 280, pp. 43017–43023.

    Article  CAS  Google Scholar 

  8. Prokhortchouk, A., Hendrich, B., Jorgensen, H., et al., Genes Dev., 2001, vol. 15, p. 13.

    Article  Google Scholar 

  9. Stogios, P.J., Downs, G.S., Jauhal, J.J., et al., Genome Biol., 2005, vol. 6.

  10. Raghav, S.K., Waszak, S.M., Krier, I., et al., Mol Cell, 2012, vol. 46, pp. 335–350.

    Article  CAS  Google Scholar 

  11. Ahanger, S.H., Shouche, Y.S., Mishra, R.K., et al., Nucleus, 2013, vol. 4, pp. 115–122.

    Article  Google Scholar 

  12. Filion, G.J., Zhenilo, S., Salozhin, S., et al., Mol. Cell Biol., 2006, vol. 1 P, pp. 169–181.

  13. Zhenilo, S., Deyev, I., Litvinova, E., et al., Cell Death Differ., 2018, vol. 11, pp. 1938–1951.

    Article  Google Scholar 

  14. Bartkuhn, M., Straub, T., Herold, M., et al., EMBO J., 2009, vol. 28, no. 7, pp. 877–888.

    Article  CAS  Google Scholar 

Download references

Funding

The study was supported by the Russian Science Foundation (project no. 19-74-30026).

Author information

Authors and Affiliations

  1. Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia

    K. Y. Khalisova,  P. G. Georgiev & A. N. Bonchuk

Authors
  1. K. Y. Khalisova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. G. Georgiev
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. N. Bonchuk
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. N. Bonchuk.

Ethics declarations

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

Additional information

Translated by M. Batrukova

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Khalisova, K.Y., Georgiev, P.G. & Bonchuk, A.N. Human CTCF Interacts with Drosophila CP190, but not with Kaiso. Dokl Biochem Biophys 497, 112–115 (2021). https://doi.org/10.1134/S1607672921020071

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1607672921020071

Keywords:

Search

Navigation