Skip to main content
SpringerLink
Log in

Leaf Anatomical Evaluation of Some Nepeta L., Taxa in Iran

  • Research Paper
  • Published:
Iranian Journal of Science and Technology, Transactions A: Science Aims and scope Submit manuscript

Abstract

We evaluated the leaf blade anatomical characteristics of thirty-three populations representing of 21 Nepeta L. taxa belonging to five sections from Iran to determine their taxonomic value in taxa delimitation and infrageneric classification in sections. Leaf samples of six individuals were examined per each population. The samples were fixed in F.A.A solution. The hand transverse sections were studied using light microscope, and data were analyzed using SPSS and MVSP softwares. We found that several leaf anatomical characteristics were common among the taxa and their populations; therefore, they had no taxonomic value. Hence, other anatomical features (the indumentum type, palisade cell wall shape, palisade/sponge cells ratio, parenchyma cells shape in midrib, the shape of vascular tissue, thickness of phloem and xylem tissues) differed among the populations and had the taxonomical importance. Moreover, the quantitative anatomical traits significantly (P ≤ 0.001) varied among the taxa and their populations. The classification pattern of the studied taxa and their populations in UPGMA tree and PCA plot was inconsistent with the infrageneric classifications of the genus according to Flora of Iran and Flora Iranica. In addition, populations of some species were clustered separately. Since there was a high level of infraspecific diversity in Nepeta taxa, this issue should be considered in all taxonomical treatments of this genus to define the correct infrageneric classification pattern.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Data Availability

The data which support the findings of this investigation are available from the corresponding author, upon reasonable request.

References

  • Abu-Assab MS, Cantino PD (1987) Phyllogenetic implications of leaf anatomy in subtribe Mellittidinñ (Labiatae) and related taxa. J Arnold Arboretum 68:1–34

    Google Scholar 

  • Acar M, Ozcan T, Satil F, Dirmenci T (2011) A comparative anatomical study on two endemic Nepeta L. species (N. baytopii and N. sorgerae). Biodivers Conserv 4(3): 58–70.

  • Araújo JS, Azevedo AA, Silva LC, Meira RMSA (2010) Leaf anatomy as an additional taxonomy tool for 16 species of Malpighiaceae found in the Cerrado area (Brazil). Plant Syst Evol 286:117–131. https://doi.org/10.1007/s00606-010-0268-3

    Article  Google Scholar 

  • Bosabalitis AM, Kokkini S (1997) Infraspecific variation of leaf anatomy in Origanum vulgare grown wild in Greece. Bot J Linn Soc 123:353–362

    Google Scholar 

  • Bukatsch F (1972) Bermerkungen zur doppelfärbung astrablau-safranin. Mikrokosmos 61:255

    Google Scholar 

  • Choi HJ, Oh BU (2011) A partial revision of Allium (Amaryllidaceae) in Korea and north-eastern China. Bot J Linn Soc 167:153–211. https://doi.org/10.1111/j.1095-8339.2011.01166.x

    Article  Google Scholar 

  • Dickison WC (2000) Integrative plant anatomy. Academic, New York

    Google Scholar 

  • Dinç M, Duran A, Pinar M, Öztürk M (2008) Anatomy, palynology and nutlet micromorphology of Turkish endemic Teucrium sandrasicum (Lamiaceae). Biologia 63(5):637–641. https://doi.org/10.2478/s11756-008-0137-5

    Article  Google Scholar 

  • Duarte MDR, Lopes JF (2007) Stem and leaf anatomy of Plectranthus neochilus Schltr. Lamiaceae. Braz J Pharmacogn 17(4):549–556

    Article  Google Scholar 

  • Ecevit-Genç G, Büyükkılıç-Altınbaşak B, Özcan T, Dirmenci T (2018) Comparative anatomical studies of some Teucrium sect. Teucrium species: Teucrium alyssifolium Stapf, Teucrium brevifolium Schreb. and Teucrium pestalozzae Boiss. (Lamiaceae). PhytoKeys 96:63–77. https://doi.org/10.3897/phytokeys.96.24498

    Article  Google Scholar 

  • Fahn A (1982) Plant anatomy. Pergamon Press, Oxford, p 545

    Google Scholar 

  • Jamzad Z (1999) Two new species of Nepeta L. (Labiatae) from Iran. Iranian J Bot 8(1):43–48

    Google Scholar 

  • Jamzad Z (2012) Flora of Iran, no. 76, Lamiaceae. Research Institute of Forest and Rangelands, Tehran

  • Jamzad Z, Assadi M (1984) New species of the genera Nepeta and Ajuga (Labiatae) from Iran. Iranian J Bot 2:95–102

    Google Scholar 

  • Jamzad Z, Chase M, Ingrouille M, Simmonds MSJ, Jalili A (2003) Phylogenetic relationships in Nepeta L. (Lamiaceae) and related genera based on ITS sequence data. Taxon 52:21–32

    Article  Google Scholar 

  • Johansen DA (1940) Plant Microtechnique. McGraw, New York

    Google Scholar 

  • Harley RM, Atkin S, Budanstev AL, Cantino PD, Conn BJ, Grayer R, Harley MM, de Tok R, Krestovskaja T, Morales R, Paton AJ, Ryding O, Upson T (2004) Flowering plants, dicotyledons. In: Kubitzki K (ed) The families and genera of vascular plants, vol 6. Springer Verlag, Berlin, pp 167–275

    Google Scholar 

  • Kahraman A, Celep F, Dogan M (2009) Comparative morphology, anatomy and palynology of two Salvia L species (Lamiaceae) and their taxonomic implications. Bangladesh J Plant Taxon 16(1):73–82. https://doi.org/10.3329/bjpt.v16i1.2749

    Article  Google Scholar 

  • Metcalfe CR, Chalk L (1979) Anatomy of dicotyledons. Vol. 1. Systematic anatomy of leaf and stem, with a brief history of the subject, 2nd edn. Clarendon Press, Oxford, pp 40–41

    Google Scholar 

  • Moon HK, Hong SP, Smets E, Huysmans S (2009) Phylogenetic significance of leaf micromorphology and anatomy in the tribe Mentheae (Nepetoideae: Lamiaceae). Bot J Linn Soc 160(2):211–231. https://doi.org/10.1111/j.1095-8339.2009.00979.x

    Article  Google Scholar 

  • Mráz P (1998) The structure and development of the glandular trichomes of Teucrium montanum (Lamiaceae). Biol Bratis 53(1):65–72

    Google Scholar 

  • Ozcan M, Eminagaoglu A (2014) Stem and leaf anatomy of three taxa in Lamiaceae. Bangladesh J Bot 43(3):345–352

    Article  Google Scholar 

  • Pădure IM (2006) Nepeta (Lamiaceae) morphology and anatomy in Romania and their taxonomic significance. Plant, fungal and habitat diversity investigation and conservation. In: Proceedings of IV BBC, Sofia. pp. 34–38

  • Pandey S, Misra S (2014) Taxonomy of angiosperms. Ane Books, New Delhi, p 620

    Google Scholar 

  • Podani J (2000) Introduction to the exploration of multivariate biological data. Backhuys Publishers, Leiden

    MATH  Google Scholar 

  • Pojarkova AI (1954) Nepeta. In: Shishkin BK, Yuzepchuk, SV (eds.), Flora of the USSR. Vol. 20. Acad Sci U.S.S.R., Moscow.

  • Rechinger KH (1982) Flora Iranica, Labiatae. Akademische Druck-U, vol 150. Verlagsanstalt, Graz

    Google Scholar 

  • Rudall P (1980) Wood anatomy in the hyptidinae (Labiatae). Kew Bull 35(4):735–741

    Article  Google Scholar 

  • Salmaki Y, Zarre S, Lindqvist C et al (2011) Comparative leaf anatomy of Stachys (Lamiaceae: Lamioideae) in Iran with a discussion on its subgeneric classification. Plant Syst Evol 294:109–125. https://doi.org/10.1007/s00606-011-0450-2

    Article  Google Scholar 

  • Talebi SM, Ghorbani-Nahooji M, Yarmoohammadi M (2017) Infra-generic morphological variations in some Nepeta L. taxa of Iran. Ukr J Ecol 7(3):208–216. https://doi.org/10.15421/2017_69

    Article  Google Scholar 

  • Talebi SM, Ghorbani Nohooji M, Yarmohammadi M (2018a) Histological and cellular variations in populations of Nepeta heliotropifolia in Iran. J Cell Tissue 8(4):387–397

    Google Scholar 

  • Talebi SM, Ghorbani-Nohooji M, Yarmohammadi M, Azizi N (2020) Pollen morphological traits analysis of eighteen Nepeta species in Iran. Mediterr Bot 41(1):85–99. https://doi.org/10.5209/mbot.62919

    Article  Google Scholar 

  • Talebi SM, Ghorbani-Nohooji M, Yarmohammadi M, Azizi N, Matsyura A (2018b) Trichomes morphology and density analysis in some Nepeta species of Iran. Mediterr Bot 39(1):51–62. https://doi.org/10.5209/MBOT.59574

    Article  Google Scholar 

  • Talebi SM, Ghorbani Nohooji M, Yarmohammadi M, Khani M, Matsyura A (2019a) Effect of altitude on essential oil composition and on glandular trichome density in three Nepeta species (N. sessilifolia, N. heliotropifolia and N. fissa). Mediterr Bot 40(1):81–93. https://doi.org/10.5209/MBOT.59730

    Article  Google Scholar 

  • Talebi SM, Sheidai M, Ariyanejad F, Matsyura A (2019b) Stem anatomical study of some Iranian Marrubium L species. Biodiversitas 20:2589–2595. https://doi.org/10.13057/biodiv/d200922

    Article  Google Scholar 

  • Yetişen K (2014) Morphological and anatomical study of the endemic species Phlomis monocephala (Lamiaceae). Phytol Balc 20(1):49–55

    Google Scholar 

Download references

Funding

The authors received no financial support for this evolution, authorship and publication of this paper.

Author information

Authors and Affiliations

  1. Department of Biology, Faculty of Sciences, Arak University, 38156-8-8349, Arak, Iran

    Seyed Mehdi Talebi

Authors
  1. Seyed Mehdi Talebi
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Seyed Mehdi Talebi designed this research, analyzes the data and wrote the manuscript.

Corresponding author

Correspondence to Seyed Mehdi Talebi.

Ethics declarations

Conflict of interest

The author declares that there is no conflict of interest.

Consent for publication

I (Seyed Mehdi Talebi) hereby declare that I participated in this research and in the development of the paper. I have read its final version and given my consent for this paper to be published in the journal.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Talebi, S.M. Leaf Anatomical Evaluation of Some Nepeta L., Taxa in Iran. Iran J Sci Technol Trans Sci 45, 1211–1222 (2021). https://doi.org/10.1007/s40995-021-01128-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40995-021-01128-x

Keywords

Search

Navigation