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A Method to Screen for Consistency of Effect in Laboratory Toxicity Tests: A Case Study with Anurans and the Herbicide Atrazine

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Abstract

This paper presents a semiquantitative method to help ecotoxicologists evaluate the consistency of data within the available peer-reviewed literature. In this case study, we queried whether there is consistent evidence of direct toxicity in Anurans exposed to atrazine at concentrations ≤ 100 μg/L under laboratory conditions. Atrazine was selected because of the relatively large repository of Anuran toxicity data. To accomplish this, we interrogated available data found in recent quantitative weight-of-evidence risk assessments for atrazine with a series of yes or no questions developed a priori. The questions examined consistency of reported effects within and between studies, within and between species, and across a wide range of endpoints categories (e.g., survivorship, growth and development, reproduction). The analysis found no compelling evidence of a consistent direct effect in Anurans around growth and development, reproduction, or survivorship at concentrations of up to at least 100 μg/L atrazine in laboratory studies. Further work is needed to refine the approach, including accounting for the magnitude of the reported effects. However, we recommend that ecotoxicologists employ some method of formal consistency of effects assessment method routinely before performing toxicity tests, in the contextualizing of new data, and in reviews of contaminants.

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References

  • Adams J, Beasley A, Belanger S, Breton R, Brock B, Buonsante VA, Burgos MG, Green J, Guiney P, Hall T, Hanson ML, Harris MJ, Henry TR, Huggett D, Junghans M, Laskowski R, Maack G, Moermond C, Panter G, Pease A, Poulsen V, Roberts M, Rudén C, Schlekat C, Schoeters I, Solomon K, Staveley J, Stubblefield W, Sumpter J, Warne MSJ, Wentsel R, Wheeler J, Wolff B, Yamazaki K, Zahner H (2019) Improving environmental risk assessments of chemicals: steps towards evidence-based ecotoxicology. Environ Int 128:210–217

    Article  Google Scholar 

  • Andrus JM, Winter D, Scanlan M, Sullivan S, Bollman W, Waggoner JB, Hosmer AJ, Brain RA (2013) Seasonal synchronicity of algal assemblages in three Midwestern agricultural streams having varying concentrations of atrazine, nutrients, and sediment. Sci Total Environ 458:125–139

    Article  Google Scholar 

  • Andrus JM, Winter D, Scanlan M, Sullivan S, Bollman W, Waggoner JB, Hosmer AJ, Brain RA (2015) Spatial and temporal variation of algal assemblages in six Midwest agricultural streams having varying levels of atrazine and other physicochemical attributes. Sci Total Environ 505:65–89

    Article  CAS  Google Scholar 

  • Ankley GT, Bennett RS, Erickson RJ, Hoff DJ, Hornung MW, Johnson RD, Mount DR, Nichols JW, Russom CL, Schmieder PK, Serrrano JA (2010) Adverse outcome pathways: a conceptual framework to support ecotoxicology research and risk assessment. Environ Toxicol Chem 29(3):730–741

    Article  CAS  Google Scholar 

  • Burden N, Benstead R, Benyon K, Clook M, Green C, Handley J, Harper N, Maynard SK, Mead C, Pearson A, Ryder K (2020) Key opportunities to replace, reduce, and refine regulatory fish acute toxicity tests. Environ Toxicol Chem 39(10):2076–2089

    Article  CAS  Google Scholar 

  • Caldwell DJ, Mastrocco F, Hutchinson TH, Länge R, Heijerick D, Janssen C, Anderson PD, Sumpter JP (2008) Derivation of an aquatic predicted no-effect concentration for the synthetic hormone, 17α-ethinyl estradiol. Environ Sci Technol 42(19):7046–7054

    Article  CAS  Google Scholar 

  • Dionne E, Hanson ML, Anderson JC, Brain RA (2021) Chronic toxicity of technical atrazine to the fathead minnow (Pimephales promelas) during a full life-cycle exposure and an evaluation of the consistency of responses. Sci Total Environ 755:142589

    Article  CAS  Google Scholar 

  • Giddings JM, Anderson TA, Hall Jr LW, Kendall RJ, Richards RP, Solomon KR, Williams WM (2005) A probabilistic aquatic ecological risk assessment of atrazine in North American surface waters. 432 pp. Pensacola, FL, USA: SETAC Press.

  • Hanson ML, Brain RA (2020) Context and perspective in ecotoxicology. Environ Toxicol Chem 39(9):1655–1655

    Article  CAS  Google Scholar 

  • Hanson ML, Deeth LE, Prosser RS (2018) Evidence of citation bias in the pesticide ecotoxicology literature. Ecotoxicology 27(7):1039–1045

    Article  CAS  Google Scholar 

  • Hanson ML, Solomon KR, Van Der Kraak G, Brain R (2019) Effects of atrazine on fish, amphibians, and reptiles: update of the analysis based on quantitative weight of evidence. Crit Rev Toxicol 49(8):670–709

    Article  CAS  Google Scholar 

  • Hill AB (1965) The environment and disease: association or causation? Proc R Soc Med 58:295–300

    CAS  Google Scholar 

  • Hutchinson TH, Wheeler JR, Gourmelon A, Burden N (2016) Promoting the 3Rs to enhance the OECD fish toxicity testing framework. Reg Toxicol Pharmacol 76:231–233

    Article  CAS  Google Scholar 

  • Jobling S, Sumpter JP, Sheahan D, Osborne JA, Matthiessen P (1996) Inhibition of testicular growth in rainbow trout (Oncorhynchus mykiss) exposed to estrogenic alkylphenolic chemicals. Environ Toxicol Chem 15(2):194–202

    Article  CAS  Google Scholar 

  • Mahler BJ, Van Metre PC, Burley TE, Loftin KA, Meyer MT, Nowell LH (2017) Similarities and differences in occurrence and temporal fluctuations in glyphosate and atrazine in small Midwestern streams (USA) during the 2013 growing season. Sci Total Environ 579:149–158

    Article  CAS  Google Scholar 

  • Raimondo S, Jackson CR, Barron MG (2010) Influence of taxonomic relatedness and chemical mode of action in acute interspecies estimation models for aquatic species. Environ Sci Technol 44(19):7711–7716

    Article  CAS  Google Scholar 

  • Rohr JR, McCoy KA (2010) A qualitative meta-analysis reveals consistent effects of atrazine on freshwater fish and amphibians. Environ Health Perspect 118:20–32

    Article  CAS  Google Scholar 

  • Rosenthal R (1979) The file drawer problem and tolerance for null results. Psychol Bull 86(3):638

    Article  Google Scholar 

  • Routledge EJ, Sheahan D, Desbrow C, Brighty GC, Waldock M, Sumpter JP (1998) Identification of estrogenic chemicals in STW effluent. 2. In vivo responses in trout and roach. Environ Sci Technol 32(11):1559–1565

    Article  CAS  Google Scholar 

  • USEPA [United States Environmental Protection Agency] (2003) A set of scientific issues being considered by the Environmental Protection Agency regarding: potential developmental effects of atrazine on amphibians. Washington, DC, USA: United States Environmental Protection Agency. Report of the FIFRA Scientific Advisory Panel Meeting, June 17–20, 2003, No. SAP Report No. 2003–01. 34 pp. www.epa.gov/scipoly/sap/index.htm

  • USEPA [United States Environmental Protection Agency (2006) Decision documents for atrazine. Washington, DC: United States Environmental Protection Agency, Office of Prevention, Pesticides, and Toxic Substances. 323 pp. www.epa.gov/oppsrrd1/REDs/atrazine_combined_docs.pdf

  • USEPA [United States Environmental Protection Agency] (2007a) FIFRA Scientific Advisory Panel Meeting, October 9–11, A set of scientific issues being considered by the Environmental Protection Agency Regarding: The Potential for Atrazine to Affect Amphibian Gonadal Development. Arlington, VA, USA: United States Environmental Protection Agency. No. FIFRA SAP Minutes No 2007–08. 32 pp. http://www.epa.gov/scipoly/SAP/meetings/2007/october/finalminutes.pdf

  • USEPA [United States Environmental Protection Agency] (2007b) White Paper on the potential for atrazine to affect amphibian gonadal development. Washington, DC, USA: United States Environmental Protection Agency. Office of Prevention, Pesticides and Toxic Substances, Office of Pesticide Programs, Environmental Fate and Effects Division. 321 pp. http://www.epa.gov/scipoly/sap/meetings/2007/october/2007_amphibian_white_paper.pdf

  • USEPA [United States Environmental Protection Agency] (2012) Meeting of the FIFRA Scientific Advisory Panel on the Problem Formulation for the Environmental Fate and Ecological Risk Assessment for Atrazine. June 12–15, 2012. https://www.regulations.gov/document?D=EPA-HQ-OPP-2012-0230-0005

  • USEPA [United States Environmental Protection Agency] (2019) Directive to Prioritize Efforts to Reduce Animal Testing. Memorandum, September 10, 2019. https://www.epa.gov/sites/production/files/2019-09/documents/image2019-09-09-231249.pdf

  • van den Berg SJ, Maltby L, Sinclair T, Liang R, van den Brink PJ (2021) Cross-species extrapolation of chemical sensitivity. Sci Total Environ 753:141800

    Article  Google Scholar 

  • Van Der Kraak GJ, Hosmer AJ, Hanson ML, Kloas W, Solomon KR (2014) Effects of atrazine in fish, amphibians, and reptiles: an analysis based on quantitative weight of evidence. Crit Rev Toxicol 44(S5):1–66

    Article  Google Scholar 

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Acknowledgments

The authors thank Julie Anderson for her assistance in collating the data used in this analysis and Syngenta LLC for funding her time to assist. They express appreciation to three anonymous reviewers and the journal’s editor for providing helpful comments that contributed to the improvement of this manuscript.

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Authors and Affiliations

  1. Department of Environment and Geography, University of Manitoba, Winnipeg, MB, Canada

    Mark L. Hanson

  2. Syngenta Crop Protection LLC, Greensboro, NC, USA

    Richard A. Brain

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  1. Mark L. Hanson
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  2. Richard A. Brain
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Correspondence to Mark L. Hanson.

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Conflict of interest

RAB is an employee of Syngenta LLC, which is the registrant of atrazine. MLH has received research support in the past from Syngenta LLC, which is the registrant of atrazine.

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Hanson, M.L., Brain, R.A. A Method to Screen for Consistency of Effect in Laboratory Toxicity Tests: A Case Study with Anurans and the Herbicide Atrazine. Arch Environ Contam Toxicol 81, 123–132 (2021). https://doi.org/10.1007/s00244-021-00847-x

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  • DOI: https://doi.org/10.1007/s00244-021-00847-x

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