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Effects of crude oil and aromatic compounds on growth and bioluminescence of Vibrio campbellii FS5

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Abstract

Fifteen native luminescent bacteria were isolated from the Gulf of Thailand, and their sensitivity for the detection of toxicity of crude oil and its aromatic components was investigated. Of these isolates, Vibrio campbellii strain FS5 was one of the two most highly inhibited bacteria at all crude oil concentrations. This bacterium showed a decrease in luminescence intensity of between 10.7 and 80.2% after a 15-min exposure to 0.0001–10 mg/L of crude oil. The degree of bioluminescence inhibition increased with increasing concentrations of crude oil. The presence of crude oil at all concentrations had negative effects on the log bioluminescence per log number of viable cells after 15- to 105-min exposure. About 10 to 100 times, lower half maximal effective concentration (EC50) values were observed for polycyclic aromatic hydrocarbons (PAHs) than those for benzene, toluene, ethylbenzene, and xylene (BTEX). In the presence of each individual BTEX and PAH, the bioluminescence inhibition increased with increasing exposure time (1–32 h). This indigenous bacterium can be used as a simple and general indicator of oil contamination and its impact on coastal waters as well as for assessing potential toxicity during oil bioremediation.

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Acknowledgements

The authors would like to thank the Center of Excellence on Hazardous Substance Management (HSM) for their invaluable support in terms of facilities and scientific equipment.

Funding

This research was supported by the Office of Higher Education Commission (OHEC) and the S&T Postgraduate Education and Research Development Office (PERDO) (contract no. HSM-PJ-CT-17–01). Support was also provided by the Postdoctoral Fellowship from Prince of Songkla University.

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Authors and Affiliations

  1. Environmental Assessment and Technology for Hazardous Waste Management Research Center, Faculty of Environmental Management, Prince of Songkla University, Songkhla, 90112, Thailand

    Suriya Palamae & Oramas Suttinun

  2. Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Penjai Sompongchaiyakul

  3. Center of Excellence On Hazardous Substance Management (HSM), Bangkok, 10330, Thailand

    Penjai Sompongchaiyakul & Oramas Suttinun

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  1. Suriya Palamae
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  2. Penjai Sompongchaiyakul
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  3. Oramas Suttinun
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Conceptualization, Methodology, Investigation, Formal analysis, Data curation, Writing—original draft: Suriya Palamae; Supervision, Funding acquisition: Penjai Sompongchaiyakul; Supervision, Conceptualization, Methodology, Resources, Data curation, Writing—original draft, Writing—review and editing, Funding acquisition: Oramas Suttinun.

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Correspondence to Oramas Suttinun.

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Palamae, S., Sompongchaiyakul, P. & Suttinun, O. Effects of crude oil and aromatic compounds on growth and bioluminescence of Vibrio campbellii FS5. Environ Monit Assess 193, 291 (2021). https://doi.org/10.1007/s10661-021-09081-3

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