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Tiller Outgrowth in Rice (Oryza sativa L.) is Controlled by OsGT1, Which Acts Downstream of FC1 in a PhyB-Independent Manner

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Abstract

Tillering is one of the most important determinants of biomass and yield in rice (Oryza sativa L.). The capacity of plants to develop tillers from primordial meristems or buds is determined not only by the genotype but also by environmental cues. Here, we characterized the function of rice grassy tiller1 (OsGT1) and its interaction with other genetic and biological factors involved in tiller bud outgrowth in rice by generating OsGT1 RNA interference (RNAi) and overexpression (OX) lines. The tiller number was increased in OsGT1-RNAi mutants but strongly suppressed in OsGT1-OX lines. Expression analysis of OsGT1 in rice phyB mutants and in genotypes carrying various genetic combinations of GT1 RNAi and phyB demonstrated that OsGT1 is not involved in phyB-mediated suppression of tiller development in rice. Expression analysis of fine culm1 (fc1), a rice tb1 homolog, and molecular assays demonstrated that FC1 enhances the expression of OsGT1 by directly binding to its promoter. Comparison of the transcriptomic profiles of fc1 and OsGT1-RNAi mutants revealed differentially expressed genes (DEGs) common to both genotypes. Finally, analysis of tillering phenotypes of OX and RNAi seedlings treated with various phytohormones implied a possible role of OsGT1 in strigolactone-mediated tiller outgrowth. Overall, this study enhances our understanding of the diverse mechanisms of tiller development in grasses.

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Acknowledgements

This research was supported by grants from the Next-Generation BioGreen 21 Program (PJ01326601), the Rural Development Administration, and from Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2020R1F1A1049807), Republic of Korea. The support of Dong-jin Kang was appreciated. C.-d. Han thanks Dong-jin Kang for help in running a lab.

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Author notes

  1. Vikranth Kumar, SungHoon Kim and MochRosyadi Adnan contributed equally to this work.

Authors and Affiliations

  1. Division of Applied Life Science (BK21 Plus Program), Plant Molecular Biology & Biotechnology Research Center (PMBBRC), Gyeongsang National University, Jinju, 52828, Republic of Korea

    Vikranth Kumar, Sung Hoon Kim, Moch Rosyadi Adnan, Jin Hee Jeong & Chang-deok Han

  2. Division of Biological Sciences, Wonkwang University, Iksan, 54538, Republic of Korea

    Jung Heo & Soon Ju Park

  3. Plasma Technology Research Center, National Fusion Research Institute, 37 Dongjangsan-ro, Gunsan, 54004, Republic of Korea

    Ryza A. Priatama

  4. Department of Plant Medicine, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 52828, Republic of Korea

    Jeung Joo Lee

  5. Division of Horticulture Industry, Wonkwang University, Iksan, 54538, Republic of Korea

    Chul Min Kim

  6. Department of Horticulture Bioscience, College of Natural Resource and Life Sciences, Pusan National University, Miryang, 50463, Republic of Korea

    Byoung Il Je

  7. College of Plant Protection, Shenyang Agricultural University, Dongling Road 120, Shenyang, 110866, China

    Yuan Hu Xuan

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  1. Vikranth Kumar
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  2. Sung Hoon Kim
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  12. Chang-deok Han
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Contributions

CDH experimental design, data analysis, and interpretation, manuscript editing. YHX experimental design, data analysis, and interpretation. VK, SHK, and MRA data generation and analysis, image analysis, data presentation, manuscript writing. RAP and JHJ material generation and analysis, DNA extraction, qPCR analysis. SJP and HJ bioinformatics analysis of RNA-seq data. CMK, BIJ, and JJL data analysis and interpretation.

Corresponding author

Correspondence to Chang-deok Han.

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Kumar, V., Kim, S.H., Adnan, M.R. et al. Tiller Outgrowth in Rice (Oryza sativa L.) is Controlled by OsGT1, Which Acts Downstream of FC1 in a PhyB-Independent Manner. J. Plant Biol. 64, 417–430 (2021). https://doi.org/10.1007/s12374-021-09310-9

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  • DOI: https://doi.org/10.1007/s12374-021-09310-9

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