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Phosphate Transporter MdPHT1;7 Enhances Phosphorus Accumulation and Improves Low Phosphorus and Drought Tolerance

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Abstract

Phosphorus is an important macronutrient for plant growth and is acquired by plants mainly as phosphate. Phosphate fertilizer is usually used to reduce inorganic phosphate (Pi) deficiency in the soil, improve the low phosphorus and drought tolerance of plants, and promote plant growth. Phosphate transporters (PHTs) play an important role in absorbing phosphorus from the soil. MdPHT1;7 was induced by Pi deficiency and drought in roots in our previous research. In this study, we cloned MdPHT1;7 and showed its heterologous expression can complement a high-affinity Pi transporter gene in the Pi uptake-defective yeast mutant BY4743. MdPHT1;7 is a phosphorus transporter located on the cell membrane. Overexpression of MdPHT1;7 in ‘Orin’ apple and ‘Micro-Tom’ tomato enhanced Pi accumulation, low Pi tolerance and drought tolerance. We hypothesized that MdPHT1;7 can enhance Pi absorption and play an important role in improving plant resistance to low phosphorus and drought stresses.

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Abbreviations

Pi:

Inorganic phosphate

PHT:

Phosphate transporter

WT:

Wild-type

OE:

Overexpression

YNB:

Yeast nitrogen base

Cef:

Cefotaxime

qRT-PCR:

Quantitative real-time PCR

EL:

Electrolyte leakage

H2O2 :

Hydrogen peroxide

MDA:

Malondialdehyde

Chl:

Chlorophyll

SOD:

Superoxide dismutase

CAT:

Catalase

ACP:

Acid phosphatase

NBT:

Nitro blue tetrazolium

DAB:

Diaminobenzidine

SD:

Standard deviation

ORFs:

Open reading frames

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31901965), and Natural Science Foundation of Inner Mongolia Autonomous Region (2019BS03037). The authors are grateful to Dr. Guomin Han at Anhui Agricultural University for providing the yeast mutant BY4743 which defects PHO84.

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Authors and Affiliations

  1. Beijing Academy of Agriculture and Forestry Sciences, Beijing Academy of Forestry and Pomology Sciences, Beijing Engineering Research Center for Deciduous Fruit Trees, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture and Rural Affairs, Beijing, 100093, China

    Tingting Sun, Beibei Zhou, Junke Zhang & Qinping Wei

  2. State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling, 712100, China

    Tingting Pei & Fengwang Ma

  3. School of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou, 014010, Inner Mongolia, China

    Tingting Sun & Hu Meng

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  1. Tingting Sun
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  2. Beibei Zhou
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  4. Hu Meng
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  5. Junke Zhang
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Contributions

FM and JZ designed the experiments. TS, BZ, TP and HM performed the experiments. TS, QW and FM wrote the manuscript with the help from other authors. All authors have read and approved the final version of the manuscript.

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Correspondence to Fengwang Ma or Qinping Wei.

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Supplementary Information

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12374_2021_9309_MOESM1_ESM.jpg

Supplementary file1 (JPG 28 KB) Fig. S1 PCR and qRT-PCR analysis of the transgenic calli of ‘Orin’ apple. (A) PCR results for Oe experiments. Lanes: L1 and L2, MdNAT7-Oe transgenic lines; M, molecular marker DL2000. (B) qRT-PCR analysis of MdPHT1;7 overexpression transcripts in L1 and L2. WT, the wild-type of apple calli; L1, L2, the different lines of MdPHT1;7-Oe transgenic callus.

12374_2021_9309_MOESM2_ESM.jpg

Supplementary file2 (JPG 24 KB) Fig. S2 PCR and qRT-PCR analysis of the MdPHT1;7 overexpression transgenic in ‘Micro-Tom’ tomato. (A) PCR results for Oe experiments. Lanes: M, molecular marker DL2000; V, positive vector containing pGWB 408-MdNAT7 plasmid; OE-1 and OE-2 transgenic lines. (B) qRT-PCR analysis of MdPHT1;7 overexpression transcripts in OE-1 and OE-2. WT, the wild-type of tomato; OE-1, OE-2, the different lines of MdPHT1;7-Oe transgenic tomato.

Supplementary file3 (DOCX 16 KB)

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Sun, T., Zhou, B., Pei, T. et al. Phosphate Transporter MdPHT1;7 Enhances Phosphorus Accumulation and Improves Low Phosphorus and Drought Tolerance. J. Plant Biol. 64, 403–416 (2021). https://doi.org/10.1007/s12374-021-09309-2

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