Abstract
Soil arbuscular mycorrhizal (AM) fungi were monitored in a 4-year experiment of nitrogen (N) and phosphorus (P) fertilization in three growing seasons (April, August, and November) under two successional stages (young and mature) in a subtropical forest of China. AM fungal extra-radical hyphal density was significantly affected by fertilization, season, and interactions between fertilization and season and between forest stage and season, but not by forest stage. The spore density of AM fungi was significantly affected by forest stage and season, but not by fertilization. Totally, 160 operational taxonomic units (OTUs) of AM fungi have been identified at a 97% sequence similarity level using Illumina MiSeq sequencing of the V4 region of the 18S rDNA. AM fungal Shannon diversity index, but not OTU richness, showed a positive response to P fertilization. The community composition of AM fungi was significantly influenced by P fertilization, but not by N fertilization, season, and forest stage. Phosphorus fertilization exhibited a negative impact on the relative abundance of the dominant Glomeraceae. This finding highlights that P availability has a strong effect on the community of AM fungi in the subtropical forest ecosystem.
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27 April 2021
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Acknowledgements
We are grateful to Mr. Zai-Gen Jiang and the staff of the Gutianshan National Nature Reserve for fertilization, field management, and sample collection.
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This work was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (grant number XDB31000000), National Natural Science Foundation of China (grant numbers 31570499, 31971447, 91751113), and CAS-TWAS President’s Fellowship (grant number 2015CTF101).
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Maitra, P., Zheng, Y., Wang, YL. et al. Phosphorus fertilization rather than nitrogen fertilization, growing season and plant successional stage structures arbuscular mycorrhizal fungal community in a subtropical forest. Biol Fertil Soils 57, 685–697 (2021). https://doi.org/10.1007/s00374-021-01554-4
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DOI: https://doi.org/10.1007/s00374-021-01554-4