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Serial Evaluation of T cell Subsets in Paediatric Acute Myeloid Leukaemia- a Prospective Study

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Indian Journal of Hematology and Blood Transfusion Aims and scope Submit manuscript

Abstract

Purpose

There is paucity of data regarding T-cells in paediatric AML patients. The aim of this prospective study was to evaluate trend of T-cell subset during disease course of paediatric AML patients and to see its correlation with patient characteristics and survival outcome.

Methods

T-cell subsets (CD3, CD4 and CD8) were evaluated by flow-cytometry at diagnosis, post-induction, post-treatment completion, at 3 months and 6 months post-treatment completion, and relapse in 29 pediatric AML patients. Trend of T-cells was plotted between group A (those in continuous remission) and group B (those who relapsed) patients.

Results

Patients with high WBC count had significantly higher number of CD3, CD4 and CD8 cell. Baseline Tcell subsets did not affect CR, EFS and OS; however, higher than median CD4 count predicted improved DFS [58% vs 25%; HR = 0.306 (0.10–0.93); P = 0.037]. On serial follow-up from post-induction till 3 months after completion of therapy, there was no difference in the absolute values of T cell subsets between group A and B patients.

Conclusion

Our study demonstrated T cell subsets are increased in AML subjects with high WBC count. CD4 cells have a positive impact on DFS. Serial follow-up has no impact on T cell subsets. Further studies in larger patient cohorts are needed to evaluate if CD4 population may serve as an immune biomarker for AML.

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Funding

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Author notes

  1. Anuj Kumar Bansal, Surender Kumar Sharawat Sharawat has contributed equally.

Authors and Affiliations

  1. Department of Medical Oncology, Dr. B.R.A. Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi, 110029, India

    Anuj Kumar Bansal, Surender Kumar Sharawat & Sameer Bakhshi

  2. Unit of Laboratory Oncology, Dr. B.R.A. Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi, 110029, India

    Ritu Gupta

  3. Department of Biostatistics, Dr. B.R.A. Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi, 110029, India

    Sreenivas Vishnubhatla

  4. Dr. B.R.A. Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi, 110029, India

    Anuj Kumar Bansal, Surender Kumar Sharawat, Ritu Gupta & Sameer Bakhshi

  5. All India Institute of Medical Sciences, New Delhi, 110029, India

    Anuj Kumar Bansal, Surender Kumar Sharawat, Sreenivas Vishnubhatla & Sameer Bakhshi

Authors
  1. Anuj Kumar Bansal
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  2. Surender Kumar Sharawat
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  3. Ritu Gupta
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  4. Sreenivas Vishnubhatla
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  5. Sameer Bakhshi
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Corresponding author

Correspondence to Sameer Bakhshi.

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Bansal, A.K., Sharawat, S.K., Gupta, R. et al. Serial Evaluation of T cell Subsets in Paediatric Acute Myeloid Leukaemia- a Prospective Study. Indian J Hematol Blood Transfus 38, 153–157 (2022). https://doi.org/10.1007/s12288-021-01436-7

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  • DOI: https://doi.org/10.1007/s12288-021-01436-7

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