Abstract
The occurrence of ischemia-reperfusion (I/R) injury leads to dysfunction as well as high rates of morbidity and mortality in stroke, and new effective therapeutic strategies for I/R are still needed. We investigated the effect of IL-27 on I/R injury-induced neurological function impairment, cerebral infarction volume and variation in levels of inflammatory factors in mice with middle cerebral artery occlusion (MCAO), as well as concentration of LDH and neuronal apoptosis in a neuron oxygen-glucose deprivation and reperfusion (OGD/R) model mediated by gp130/STAT3 signaling in vitro. Our results indicated that IL-27 could bind to its receptor of gp130 to attenuate the I/R injury-induced impairment function and cerebral infarction volume, and decrease inflammatory cytokines TNF-α, IL-1β and MCP-1 but increase anti-inflammatory factors IL-10 and TGF-β in vivo, while inhibiting LDH leakage and neuronal apoptosis through activation of STAT3 to antagonize I/R induction. Our results suggest that IL-27 may protect the brain from I/R injury through the gp130/STAT3 signaling pathway.
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All the data involved in this study are included along with this article.
Abbreviations
- MCAO:
-
Middle cerebral artery occlusion
- OGD/R:
-
Oxygen-glucose deprivation and reperfusion
- I/R:
-
Ischemia–reperfusion
- IL-27:
-
Interleukin 27
- STAT3:
-
Activator of signal transcriptional and activators transcription3
- p-STAT3:
-
Phosphorylation STAT3
- TTC:
-
Triphenyl tetrazolium chloride
- DAPI:
-
4’,6’-Diamidino-2-phenylindole dihydrochloride
- c-IAP2:
-
Inhibitor of apoptosis protein-2
- LDH:
-
Lactate dehydrogenase
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Acknowledgements
This research was supported by funding from the project of science and technology plan Chongzuo (Grant No. 2019009 ChongKeGong).
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Chun Luo: conceptualization, methodology, writing, visualization; Binru Li: data curation, investigation; Lang Chen: data curation, validation; Lili Zhao: investigation; Yinghai Wei: writing, reviewing, editing supervision.
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Luo, C., Li, B., Chen, L. et al. IL-27 Protects the Brain from Ischemia-Reperfusion Injury via the gp130/STAT3 Signaling Pathway. J Mol Neurosci 71, 1838–1848 (2021). https://doi.org/10.1007/s12031-021-01802-0
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DOI: https://doi.org/10.1007/s12031-021-01802-0