FormalPara Key Points

In a retrospective cohort of 185 patients with multiple sclerosis treated with ocrelizumab, 176 infections were reported, in 46.1% of patients.

Odds of infection in univariate and multivariable analyses were not uniform, with increasing IgA and IgG and multiple clinical factors being associated with reduced odds of infection.

1 Introduction

Multiple sclerosis (MS) is a chronic inflammatory demyelinating disorder of the central nervous system, and a foremost cause of disability in young adults. Highly effective disease modifying therapies (DMTs) in relapsing-remitting MS (RRMS) have shown superior efficacy in reducing the rate of clinical relapses, MRI activity and disability accumulation in randomised clinical trials (RCTs). Observational data has shown that initial treatment with higher efficacy DMTs is associated with a more favourable long-term Expanded Disability Status Scale (EDSS) score [1, 2] and a lower risk of conversion to secondary-progressive MS (SPMS) [3]. Use of higher efficacy DMTs is chiefly balanced against increased risk of serious adverse events, particularly risk of infection. Compared with the general population, patients with MS have been shown to have higher rates of infection [4] and infection-related health-care utilisation, including hospitalisation [4, 5], and are more likely to die from infectious causes [6,7,8]. DMTs appear to be associated with varying infection risk, with real-world data indicating a higher risk of infection-related hospitalisation for fingolimod, natalizumab and rituximab compared with both the general population and MS patients treated with injectable therapies [9]. Also, higher rates of infections were seen in MS patients on natalizumab and fingolimod compared with MS patients not exposed to DMTs [10].

While MS is traditionally thought to be a T-cell-mediated disease, B-cells are now recognised as integral to the pathophysiology of MS, as evidenced by (1) intrathecal oligoclonal band synthesis, (2) B-cell detection in meningeal aggregates and leptomeningeal lymphoid follicles, (3) B-cells in perivascular lesions, and (4) the high efficacy of B-cell depleting therapies [11, 12], as demonstrated in the pivotal phase II and III studies in RRMS [13,14,15] and primary progressive MS (PPMS) [16].

In phase III randomised trials, ocrelizumab, a fully humanised anti-CD20 monoclonal antibody, was not associated with higher rates of serious infections compared with interferon-β in RRMS [14] and placebo in PPMS [16]. Higher rates of upper respiratory tract infection and herpes infections, however, were seen in ocrelizumab-treated patients in both studies. Further, a higher rate of nasopharyngitis was observed in ocrelizumab-treated patients compared with interferon-β in the RRMS groups [14, 16]. On the other hand, use of ocrelizumab in rheumatoid arthritis in combination with methotrexate resulted in an increased rate of serious infections compared with methotrexate use alone [17]. Emerging data has indicated a possible association between B-cell depleting therapies and more severe COVID-19 outcomes [18, 19], engendering clinical uncertainty with regards to initiating, continuing or postponing treatment with B-cell depleting therapies in patients with MS.

Identifying patients who may be at higher risk of infection when treated with ocrelizumab and other B-cell depleting DMTs is therefore an important consideration. The primary aim of this study was to identify risk factors for infection of any kind in patients with MS treated with ocrelizumab in a tertiary health centre in Victoria, Australia.

2 Methods

2.1 Study Design

In a single-centre, retrospective, observational cohort study of patients with MS treated with ocrelizumab, from 1 April until 2 October 2020, we evaluated the number of infections reported by patients since their prior infusion, either via voluntary survey completed by patients upon attending hospital for their ocrelizumab infusions, or via a phone survey following informed consent. The study received ethics approval from The Royal Melbourne Hospital Human Research Ethics Committee (QA2020045).

Patients were asked to note if they had any upper or lower respiratory tract infections, flu-like illness, herpes mucocutaneous lesions (including herpes zoster), urinary tract infections, gastrointestinal infection, cellulitis, or other infections, by either selecting pre-defined descriptions of symptoms from a list, or detailing their infective symptoms (see supplementary appendix in the electronic supplementary material [ESM]), also listing the number of such infections to a maximum of four for a given type, except herpes labialis, for which the maximum number in a given patient was counted as one, given such infections were deemed most likely to be prone to recall bias. They were also asked if antimicrobial use was initiated for a given infection. The reported interval of interest was the time between the most recent two infusions of ocrelizumab (or the time of completing a phone survey where an infusion was deferred).

Further, patients were asked to report if their rate of infection had remained unaltered, increased or decreased since commencing ocrelizumab, and to report influenza vaccination administered since their last infusion. All patients were also asked about smoking status.

Where possible, external general practice records (from their primary care physician) were obtained to confirm infection-related health-care consultations and antimicrobial utilisation pertaining to infections outlined by patients in their survey, and hospital records were used to identify any admissions due to infection in the corresponding time period, as well as potential infusion-related reactions (IRR) that could confound interpretation of relevant infections as reported by patients.

2.2 Data Acquisition

Variables identified for analysis as potential contributors to infection risk included age, sex, MS duration, EDSS, number of ocrelizumab cycles and prior immunosuppression (denoting any prior DMT other than interferon-β, glatiramer acetate and intravenous immunoglobulin [IVIg]), IgG, IgA and IgM levels, total lymphocyte levels, neutrophil counts and selected lymphocyte subsets. In parallel to evaluating risk of infection, risk of antimicrobial use was elaborated. A composite variable of self-reported or confirmed antimicrobial use was developed to approximate the proportion of patients sustaining infections deemed to require antimicrobials. In addition to patients self-reporting antimicrobial use, a proportion of patients not reporting antimicrobial use reported a health-care visit for an infection, and where subsequent review of relevant medical records revealed prescription of an antimicrobial agent, such cases were included in the composite variable. This was thought overall to reflect a more accurate representation of the proportion of patients prescribed antimicrobials.

Baseline demographic data was obtained from iMed, an MS clinical data entry system. Electronic medical records and laboratory data on white cell count differentials, lymphocyte subsets and immunoglobulin levels were obtained from the Royal Melbourne Hospital electronic medical records system and external pathology electronic records. Lymphocyte subsets were measured and quantified as follows: total lymphocyte count and absolute counts of CD3+, CD4+, CD8+, and CD19+.

2.3 Statistical Analysis

Statistical analysis was carried out using R version 1.2.5033. Means and standard deviations (SDs) were obtained where appropriate to summarise demographic clinical and laboratory data. Logistic regression models within the generalised linear model framework were used to perform univariate and multivariable analyses, to estimate the associations of selected clinical and laboratory variables with the outcomes of self-reported infection and self-reported or confirmed antimicrobial use. Accordingly, odds ratios (ORs) and 95% confidence intervals (95% CI) were calculated. Variables selected for multivariable analyses included those with p < 0.05 in the univariate analyses, and variables deemed important clinical confounders of infection risk, specifically, age, gender, EDSS score, disease duration, number of ocrelizumab cycles and respective immunoglobulin levels.

To account for the effect of the variability in the reported interval for infections, the logarithm of the time from a patient’s prior infusion until the time of the survey was included as an offset in each of the logistic regression models.

The timing of pre-infusion lymphocyte and immunoglobulin measurements was subject to variation. Given the propensity for reconstitution of lymphocyte and immunoglobulin levels with time since CD20+ depletion, a binary correction variable (1 for patients who had a blood test after the median time interval since their prior infusion [5.8 months] and 0 otherwise) was included in all the models involving a laboratory variable.

Immunoglobulin data were only available for subgroups of the study cohort. Therefore, a multivariable model was run separately for IgG, IgM and IgA within these respective subgroups.

3 Results

3.1 Demographic Results

Of 237 patients receiving infusions from April 1, 2020 until October 12, 2020, 207 completed the survey. Of these, 22 patients who were receiving their first dose of ocrelizumab were excluded. In total, 185 patients with MS were included in the study, with 126 completing the survey individually at the time of their most recent infusion, and a further 59 patients completing the survey via phone. Demographics and clinical characteristics are outlined in Table 1.

Table 1 Demographics and clinical characteristics

In brief, the mean (SD) age was 43.4 (10.6) years, 134 of 185 (72.4%) were female, the mean (SD) disease duration was 10.7 (7.5) years, 50 (29.4%) patients were DMT-naïve prior to ocrelizumab, the median (1st, 3rd quartiles) EDSS score was 2.0 (1, 4) and the mean (SD) number of ocrelizumab doses was 4.6 (1.3). One hundred patients who had transitioned off a prior DMT had washout information available. The number of patients with available intervals and the median washout (range) for respective DMTs were as follows: natalizumab: 52 patients, 42.5 (16–446) days; fingolimod: 32 patients, 41.0 (16–125) days; rituximab: 11 patients, 189.0 (117–227) days; dimethyl fumarate: 2 patients, 23.5 (8–39) days; alemtuzumab: 2 patients, 742.5 (604–881) days; and daclizumab: 1 patient, 33.0 days.

Immunoglobulin levels for analysis were available as follows: 131 patients had IgG levels, 98 had IgA levels and 107 had available IgM levels. Of patients reporting infections, available immunoglobulin levels were as follows: 68 of 89 (76%) for IgG, 65 (73%) for IgA and 66 (74%) for IgM. Mean immunoglobulin levels of IgG, IgA and IgM were 9.54 ± 2.40 g/L, 1.80 ± 0.92 g/L and 0.70 ± 0.48 g/L, respectively, with 2.3%, 3.1% and 9.3% of patients, respectively, recording levels below the lower limit of normal prior to their index infusion. The mean lymphocyte count was 1.67 ± 0.70 × 109/L, mean neutrophil count was 4.57 ± 1.92 × 109/L and mean CD4+, CD8+ and CD19+ counts were 0.93 ± 0.51 × 109/L, 0.40 ± 0.27 × 109/L and 0.02 ± 0.06 × 109/L, respectively.

3.2 Infections, Health-Care Utilisation, Hospitalisation

The total number of self-reported infections was 176, in 89 (48.1%) patients. Upper respiratory tract infections (78 total in 27.6% of patients) were the most common, followed by urinary tract infections (33 total in 11.9% of patients). No patients contracted SARS-CoV-2. Further details on the type of reported infections and frequency are outlined in Table 2. The rate of self-reported infections was 169.4 per 100 patient years (PY). Of these, 47 patients reported antimicrobial prescription and 27 had confirmed infection-related health-care visits, totalling 45 visits, with prescription of antimicrobials confirmed on 39 occasions, with a rate of prescription of 37.5 per 100 PY. Five (2.7%) patients were hospitalised, with hospital diagnoses of a viral infection with superimposed bacterial infection, osteomyelitis, urosepsis, cystitis and pyelonephritis. Details as to the clinical characteristics of these patients can be found in the ESM. The majority of patients (72%) reported no change in the frequency of infection since commencing ocrelizumab, with 16% perceiving an increased frequency and 12% a decreased frequency.

Table 2 Self-reported infections

On review of medical records in the relevant reporting period, in patients reporting respiratory symptoms, documentation of a likely IRR was confirmed in one patient contemporaneous with their infusion (feeling flushed, noted to be tachycardic), but in this patient an upper respiratory infection was confirmed via external health records corresponding to a distant future date. Three other patients reported symptoms during their ocrelizumab infusions, thought unlikely to confound interpretation of descriptions of subsequent infections—in brief, self-limiting dizziness in one patient, and worsening fatigue in two other patients.

3.3 Univariate, Multivariable Analyses of Predictors of Infection and Antimicrobial Use

In the univariate analyses (Table 3), only higher serum IgA showed a statistically significant association with decreased odds of infection (OR 0.44, 95% CI 0.25–0.76). In a clinical multivariable model (n = 139, Table 4), we did not find evidence for independent associations between any of age, gender, disease duration, doses of ocrelizumab and EDSS score and self-reported infection risk. In a separate multivariable model, higher IgA levels (n = 73) were associated with lower odds of infection (OR 0.37, 95% CI 0.17–0.80). In a multivariable model evaluating IgG and the prior clinical variables (n = 104), older age (OR 0.94, 95% CI 0.89–0.99), and higher serum IgG (OR 0.81, 95% CI 0.67–0.99) were associated with lower odds of infection. Finally, in a multivariable model including IgM levels (n = 83, Table 4), only older age was associated with lower odds of infection (OR 0.93, 95% CI 0.88–0.99).

Table 3 Univariate analysis of the predictors of self-reported infection
Table 4 Multivariable analyses of the predictors of self-reported infection.

The univariate analyses (Table 5) did not identify any variables showing a significant association with use of antimicrobial therapy. In a clinical multivariable model, only older age was associated with lower odds of antimicrobial therapy use (OR 0.95, 95% CI 0.91–1.00). In three further multivariable models (Table 6), older age and shorter disease duration were associated with a lower risk of using antimicrobial therapy. In one model, lower EDSS (OR 1.99, 95% CI 1.02–3.86) and higher IgA levels (OR 0.23, 95% CI 0.07–0.79) were associated with lower odds of antimicrobial use (n = 73). We did not find evidence for associations of IgG levels (n = 104, OR 0.82, 95% CI 0.65–1.04) and IgM levels (n = 83, OR 0.12, 95% CI 0.10–1.50) with antimicrobial use.

Table 5 Univariate analysis of the predictors of self-reported and confirmed antimicrobial use
Table 6 Multivariable analyses of the predictors of self-reported and confirmed antimicrobial use

4 Discussion

This study demonstrated that, in multivariable models, higher serum IgA and IgG and younger age were associated with reduced odds of infection. Further, younger age, longer MS disease duration and increased EDSS were associated with increased odds of antimicrobial use, whilst higher serum IgA was associated with reduced odds of antimicrobial use. Our study builds on literature detailing safety outcomes of a B-cell depleting therapy, ocrelizumab, whilst revealing novel factors that could predispose to heightened infection risk and increased infection-related treatment.

The overall rate of self-reported infections in our study of 169.4 per 100 PY was higher than the reported rate of infections in recently published 5-year data from the OPERA double-blind and open-label extension (OLE) studies in RRMS patients [20]. In our study, 16% of patients were > 55 years of age, and 11% of patients had an EDSS score > 5.5, reflecting the greater clinical heterogeneity encountered in a real-world setting. Whilst a direct comparison to the aforementioned findings is not possible given the different methods of defining infections, our findings highlight the possibility of higher rates of infection than seen in selected clinical trial populations. Notwithstanding, older age in our cohort was not associated with increased risk of infection.

The most commonly reported infection in our cohort was that involving the upper respiratory tract (78 cases in 27.6% of patients), followed by urinary tract infection (33 cases in 11.9% of patients), herpes labialis, mouth or genital ulcers (14 cases in 7.6% of patients). Review of relevant medical records failed to identify significant IRRs in patients in whom dates of respiratory infections were unknown, thereby potentially confounding interpretation of relevant reported symptoms, though it should be noted that after hospital discharge following an infusion, medical records would only reflect IRRs in patients who had reported attributable symptoms. The ensuing rate of healthcare-related visits that could be confirmed was 43.3 per 100 PY, and the rate of confirmed antimicrobial use alone was 37.5 per 100 PY. With the exception of two prescriptions of famciclovir for cutaneous herpes simplex virus (HSV), antimicrobials were exclusively represented by antibiotic prescription (a full list of confirmed antimicrobials is listed in the ESM). This represented a higher rate of antibiotic prescription than previously published data for rituximab, a chimeric anti-CD20 monoclonal antibody, in a national register [9], although it should be noted our cohort was significantly smaller in size. Similarly, the higher rate of serious infection seen in our study compared with the OPERA-OLE [20] study likewise needs to be interpreted with caution, given the low number of serious infections (5).

The association of younger age with risk of infection and antimicrobial use was an unexpected finding. Of patients younger than the median age of 42.58 (data not shown), 52.7% (48 of 91) reported an infection, compared with 43.64% (41 of 94) in those at this age or greater. In the younger group, 34.1% of patients (31 of 91) reported an upper respiratory tract infection, whereas in the older group, 21.3% of patients (20 of 94) reported an upper respiratory tract infection, accounting for the largest proportion of the difference in total frequency of infections between these groups. Urinary tract infections, occurring in 13.2% (12 of 91) and 10.6% (10 of 94) of patients in the younger and older age groups, respectively, were more evenly balanced. The higher rate of infection (and particularly upper respiratory tract infections) reported in younger patients we felt is unlikely attributable exclusively to age, but rather, may have been influenced by factors such as exposure to young children, and older patients potentially isolating more stringently given public awareness of the heightened risk for severe COVID-19 with increasing age. A differential effect of recall bias cannot be excluded.

The association of antimicrobial use with increasing disease duration seen in our study represents a novel finding. Greater cumulative exposure to DMTs with longer disease duration, and greater likelihood of having medical comorbidities may underpin this relationship. Cardiac and pulmonary disease, for instance, have been associated with increased risk of severe infection in rituximab-treated patients [21]. Indeed, in the era of COVID-19, medical comorbidities are associated with greater COVID-19 severity in the general population [22, 23] and patients with MS [23,24,25]; it thus follows that patients with CD20 depletion and medical comorbidities may have a heightened risk of infection compared with those without.

Increasing age and EDSS score are likewise associated with greater COVID-19 severity in patients with MS [24, 25], and along with disease duration, are more broadly associated with risk of infection-related hospitalisation in MS patients [26]. In our study, consistent with these findings, increasing EDSS was associated with an increased risk of antimicrobial use. Higher EDSS is associated with more frequent urological complications, including infection [27], with lower urinary tract dysfunction, catheterisation, and greater functional dependence leading to compromised hygiene, all potentially increasing the risk of urinary tract infections [27]. Likewise, respiratory dysfunction is associated with higher EDSS in patients with MS [28], predisposing to aspiration pneumonia and lung infections [29].

No relationship between prior immunosuppression and infection or antimicrobial use was seen in our study, but given the heterogeneous levels of immune suppression associated with these agents as a group, variable wash-out periods and variable subsequent duration of ocrelizumab use, a more meaningful analysis was precluded. Future studies could explore the differential effect of prior DMTs on risk of infection in ocrelizumab-treated patients with larger numbers of patients in respective groups.

In our cohort, given only small proportions of progressive phenotypes—with 14 patients in total (7.6%) with either PPMS or SPMS—the impact of MS course on infection risk could not be fully explored, though an association between higher EDSS and antimicrobial use was seen. In the phase III RCT, serious infections occurred in a similar number of patients receiving ocrelizumab as compared with placebo [16] and remained balanced in the OLE [30]. Conversely, in rituximab-treated PPMS patients, in the phase II/III RCT, serious infections occurred compared with in those receiving placebo. This disparity may reflect a higher mean age and EDSS in PPMS populations than is typically seen in RRMS [31].

The association of lower serum IgG levels and risk of infection as seen in our study is consistent with findings from phase III ocrelizumab OLE data, with significant associations of IgG and IgM but not IgA levels below the lower limit of normal and serious infections observed [32]. Rituximab-related hypogammaglobulinaemia in neuromyelitis optica spectrum disorder is similarly associated with serious infection [33]. Conversely, in an MS cohort treated with ocrelizumab [34], neither IgG nor IgM predicted the risk of infection during a 26-month median follow-up. A study of rituximab-treated rheumatoid arthritis patients with concurrent methotrexate similarly demonstrated that rates of serious infection did not differ before and after the development of IgG or IgM below the lower limit of normal, though the minimum follow-up was 4 months [35]. These findings may underscore the need for longer follow-up to better delineate the consequence of B-cell depletion mediated hypogammaglobulinaemia on infection risk. Indeed, OLE data for ocrelizumab in RRMS [20] and PPMS [30] demonstrated similar increases in the proportions of patients developing IgG, IgA and IgM below the lower limit of normal over time, consistent with data in rheumatological [35, 36] and other diseases [37] in demonstrating a relationship between the number of doses of anti-CD20 therapy and risk of hypogammaglobulinaemia.

The association of lower serum IgA in our study and the risk of infection represents an uncommonly reported finding. Reduced serum IgA in the context of B-cell depletion has generally not been considered as a risk factor for infection [38]. The majority of patients with primary IgA deficiency are asymptomatic, though some patients have a propensity to recurrent sinopulmonary infections [39]. Lower serum IgA levels may be a surrogate marker of underlying immune perturbation, indirectly contributing to infection risk. The reduction of serum IgG and IgA post B-cell depletion remains incompletely understood, given long-lived plasma cells, which do not express CD20 [40], appear to be impervious to CD20-depleting therapy [41]. Mechanisms may include a reduced pool of memory B-cells to differentiate into plasmablasts and plasma cells [42]. This, however, could only partly explain it, given that, in mouse models, memory B-cells are not required to maintain bone marrow plasma cells [41, 43]. The more common occurrence of IgM hypogammaglobulinaemia following treatment with anti-CD20 therapy may likewise reflect a dependence of IgM-secreting plasma cells on a pool of memory B-cells, though this in itself may be incompletely understood, given the presence of long-lived IgM plasma cells [44]. In addition to clinical significance, mechanisms of anti-CD20-related hypogammaglobulinaemia also require future elucidation.

Furthermore, beyond the scope of our study, owing to a lack of available data, multiple studies have implicated pre-CD20 therapy hypogammaglobulinaemia as a risk factor for future infections [21, 35], fostering the recommendation to assess patients’ immunoglobulin levels prior to CD20 therapy initiation, which is often not done in clinical practice [37].

No association was seen in our study between total lymphocyte count and lymphocyte subset levels and risk of self-reported infection or antimicrobial use. Considering CD19+ levels, given the profoundly reduced mean count in our cohort, likely due to the majority of patients having undetectable levels, an association between B-cell depletion and infection risk, even if present, could not be borne out. In ocrelizumab-treated patients with PPMS [16], a reduction in CD8+ T cells and an initial decrease in CD3+ cells have been demonstrated. In patients with MS, both rituximab [45] and ocrelizumab [46] have been shown to deplete CD20+ T-cells, which comprise a minority of overall T-cells, and are thought to represent a highly activated T-cell population with pro-inflammatory properties [46]. Nonetheless, the significance of these findings requires further elaboration, and the effect of CD3+CD20+ depletion in the context of CD20+ depletion, if any, on risk of infection in clinical practice remains unknown.

Similarly, no relationship was seen between neutrophil counts and self-reported infections or anti-microbial use. In part, this may be reflective of the generally robust absolute neutrophil counts (ANC) in our study, with only two patients having a grade 1 neutropaenia (data not shown). In the phase III PPMS ocrelizumab study, 1% of patients developed neutrophil counts < 1.0 × 109/L, without associated infections [47]. Furthermore, consistent with the rare association in relation to rituximab therapy, late-onset neutropaenia, described as an ANC below 1.5 × 109/L, > 4 weeks after the last dose of therapy [48], has been reported in MS patients receiving ocrelizumab, without any infectious complications [49,50,51]. Given the rarity of anti-CD20-related neutropaenia, larger studies with longer follow-up would be of interest to explore the incidence, infective risks and management implications.

During the study period, three patients discontinued ocrelizumab. One was a patient hospitalised with a diagnosis of a viral infection with superimposed bacterial infection, who had been deemed to have experienced frequent infections longitudinally whilst receiving ocrelizumab; another was a patient admitted with osteomyelitis, and in another patient, ocrelizumab was discontinued due to subclinical IgG hypogammaglobulinaemia. Historical patients in our institution to have discontinued ocrelizumab prior to our study period were excluded to avoid increased recall bias in assessing infections not reflected in medical records.

Given the low proportion of patients who were current smokers (15.1%), and the potential impact of COVID-19 public health measures on rates of influenza transmission, smoking and influenza vaccination were not considered in our analyses as potential factors modulating infection risk. Of seven patients reporting an influenza-like illness, three had received the seasonal influenza vaccine. None of the patients who received the pneumococcal vaccine reported symptoms suggestive of lower respiratory tract infections.

Notwithstanding the reported infections, reassuringly, only 16% of patients reported an increase in the frequency of infections sustained whilst receiving ocrelizumab. Further studies are required to evaluate the head-to-head difference in rate of infection in ocrelizumab-treated patients and those on other DMTs, particularly other higher efficacy DMTs.

Our work adds to the body of literature detailing real-world outcomes in MS patients treated with ocrelizumab and safety outcomes. In comparison with further previously published real-world series [52,53,54,55,56], our reported frequency of infections was significantly higher; although, with the exception of the incorporation of phone interviews in one study [52], these studies largely included infections as they were recorded in medical records. Notwithstanding the possibility of greater accuracy in defining infections, a review of medical records alone may under-report the true incidence, particularly for milder infections, which are less likely to be raised in standard outpatient consultations. Interestingly, few infection-related hospitalisations were reported; although, similar to our study, studies were limited to the initial cycles of ocrelizumab treatment, with a median of three infusions in two studies [55, 56], and a median follow-up period of 200 days after initiation in another study [53]. Thus, particularly considering the increasing incidence of hypogammaglobulinaemia with longer treatment duration, the need for longer-term data remains. Indeed, a large, prospective real-world study evaluating safety outcomes, including infection, in ocrelizumab-treated MS patients is currently under way [57]. Further, where assessed, in one study, a higher rate of adverse events were seen in patients previously treated with DMTs, though this was not statistically significant, and the absolute numbers of infections were low [56]. Beyond this, authors generally concluded that there were no relationships between baseline demographics and infection risk [52, 55], and though an overall and pre- and post-treatment decline in IgG was seen in one study, conclusions couldn’t be drawn as to the relationship of these findings with risk of infection [52]. Finally, in a comparison of the tolerability and immune effects of ocrelizumab and rituximab in MS [58], reductions in IgG levels in ocrelizumab-treated patients and IgM levels in both groups were seen, but the significance of these findings with respect to infection was not assessed.

Our study has a number of limitations. The retrospective manner of data collection raises the possibility of recall and reporting bias, with the possibility that reports of infection and antimicrobial use could be either over- or under-reported. Further, it is possible patient-completed and phone-completed surveys may have been associated with varying degrees of recall bias. Given the pre-defined descriptions of infection, an accurate and proven diagnosis of a given type of infection was not possible. Infections being defined via self-reported survey prevents comparison of our findings with studies reporting infection based on a clinical assessment and diagnosis. Further, our study evaluated patients at a single time point, focusing on the rates of infection ‘since’ their last infusion. Longer duration follow-up may have provided a better reflection of possible associations between clinical factors and infection risk, but at risk of further increasing recall bias. A lack of centralised health-care records meant it was not possible to verify all infections where a patient had a medical assessment, as patients would often attend a different general practice to that listed in hospital records or may attend multiple practices. Considering antimicrobial usage, limiting this variable to those in whom antimicrobial use could be confirmed by review of medical records alone was deemed to significantly underestimate the true proportion of such patients, given the aforementioned difficulty in accessing all relevant records.

Due to COVID-19 public health measures such mask-wearing in public, restrictions upon leaving one’s home and social distancing, the frequency of airborne and droplet-transmitted respiratory infections may have been reduced for a significant proportion of our study. Also, we were not able to account for patients’ medical comorbidities, which may in turn have independent associations with risk of infection. Laboratory measurements in our cohort were obtained from multiple laboratories, which could have influenced immunoglobulin and lymphocyte results, and measurements were not consistently available for each patient, as previously detailed.

Finally, due to the limited sample size, clinically meaningful associations may have been missed in the analyses. We were only able to assess linear relationships between the predictors and the outcomes.

5 Conclusions

Overall, our study demonstrated that in a large cohort of MS patients receiving ocrelizumab, higher serum IgG and IgA levels and older age were associated with a reduced odds of infection. Our study also showed that older age and higher EDSS were associated with reduced odds of antimicrobial use, and longer disease duration and higher EDSS were associated with an increased odds of antimicrobial use. Although some of these factors are well described predicators, serum IgA levels and risk of infection in ocrelizumab-treated patients is a novel finding. Our results highlight the need to monitor patients’ immunoglobulin profiles prior to commencing ocrelizumab and during the maintenance course. Further studies are required to delineate the relationship of immunoglobulin levels and infection risk, and more broadly, assess long-term risks factors for infection in patients receiving ocrelizumab.