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Diversity of fungal DNA in lake sediments on Vega Island, north-east Antarctic Peninsula assessed using DNA metabarcoding

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Abstract

We assessed the diversity of fungal DNA present in sediments of three lakes on Vega Island, north-east Antarctic Peninsula using metabarcoding through high-throughput sequencing (HTS). A total of 640,902 fungal DNA reads were detected, which were assigned to 224 taxa of the phyla Ascomycota, Rozellomycota, Basidiomycota, Chytridiomycota and Mortierellomycota, in rank order of abundance. The most abundant genera were Pseudogymnoascus, Penicillium and Mortierella. However, a majority (423,508, 66%) of the reads, representing by 43 ASVs, could only be assigned at higher taxonomic levels and may represent taxa not currently included in the sequence databases used or be new or previously unreported taxa present in Antarctic lakes. The three lakes were characterized by high sequence diversity, richness, and moderate dominance indices. The ASVs were dominated by psychrotolerant and cosmopolitan cold-adapted Ascomycota, Basidiomycota and Mortierellomycota commonly reported in Antarctic environments. However, other taxa detected included unidentified members of Rozellomycota and Chytridiomycota species not previously reported in Antarctic lakes. The assigned diversity was composed mainly of taxa recognized as decomposers and pathogens of plants and invertebrates.

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Acknowledgements

This study received financial support from CNPq, PROANTAR, FAPEMIG, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil (CAPES), INCT Criosfera 2. P. Convey is supported by NERC core funding to the British Antarctic Survey’s ‘Biodiversity, Evolution and Adaptation’ Team. We thank the Instituto Antártico Argentino for logistical and financial support for the field campaign in Vega Island, the Lagos team field group, and Fernando Calabozo for the photograph used in Fig. 1d.

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Authors and Affiliations

  1. Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, P. O. Box 486, Belo Horizonte, MG, 31270-901, Brazil

    Mayara Baptistucci Ogaki, Carlos Augusto Rosa & Luiz Henrique Rosa

  2. Departamento de Botânica, Universidade de Brasília, Brasilia, Brazil

    Paulo Eduardo Aguiar Saraiva Câmara & Micheline Carvalho-Silva

  3. Departamento de Biologia Celular, Universidade de Brasília, Brasilia, Brazil

    Otávio Henrique Bezerra Pinto

  4. Instituto Antártico Argentino, Buenos Aires, Argentina

    Juan Manuel Lirio & Silvia H. Coria

  5. Instituto de Geociências, Universidade Federal Fluminense, Niteroi, RJ, Brazil

    Rosemary Vieira

  6. British Antarctic Survey, NERC, High Cross, Madingley Road, Cambridge, CB3 0ET, UK

    Peter Convey

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  1. Mayara Baptistucci Ogaki
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Correspondence to Luiz Henrique Rosa.

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Ogaki, M.B., Câmara, P.E.A.S., Pinto, O.H.B. et al. Diversity of fungal DNA in lake sediments on Vega Island, north-east Antarctic Peninsula assessed using DNA metabarcoding. Extremophiles 25, 257–265 (2021). https://doi.org/10.1007/s00792-021-01226-z

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