Abstract
An aqueous extract was obtained from the biomass of Hermetia illucens larvae using the electro-pulse plasma-dynamic method. The extract was highly effective in quenching the chemiluminescence of luminol in the presence of hemoglobin and hydrogen peroxide. It contained the dark pigment melanin with a concentration of paramagnetic centers of 8.3 × 1016 spin/g of dry matter and antioxidant activity. The antiradical properties of the extract were confirmed on the model of oxidative stress of the nematode Caenorhabditis elegans in the presence of 40 mmol of paraquat: the introduction of the extract increased the median lifespan of the nematode by 25%. The antioxidant effect identified is associated with the presence of a complex of antioxidants in the extract and, possibly, antioxidant enzymes that destroy hydroperoxides.
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ACKNOWLEDGMENTS
The authors express their gratitude to the General Director of LLC KorolevFarm N.S. Nuzhnenko and Director of Production of LLC KorolevFarm Yu.M. Svetushkina for obtaining the extract by the method of EPDE.
Funding
This work was supported by the Ministry of Education and Science, project no. 075-11-2019-070, and as part of a State Assignment for topic no. 0109-2019-0008.
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Statement on the welfare of animals. This work was carried out in compliance with the ethical standards of scientific experiments with animals (Order of the USSR Ministry of Health No. 755 from August 12, 1977. On Measures to Further Improve Organizational Forms of Work with the Use of Experimental Animals).
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Ushakova, N.A., Dontsov, A.E., Marsova, M.V. et al. Antioxidant Properties of an Extract of Hermetia illucens Larvae. Biol Bull Russ Acad Sci 48, 118–121 (2021). https://doi.org/10.1134/S1062359021020138
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DOI: https://doi.org/10.1134/S1062359021020138