Abstract
Nile tilapia is an important economically farmed fish worldwide, and Streptococcus agalactiae has become the most serious disease problem for the tilapia industry in China. Therefore, a better understanding of the innate immune mechanisms and their functions in the context of pathogen invasion is needed. In this study, a high-throughput proteomic strategy was developed for determining the differential expression proteins (DEPs) of the genetically improved farmed tilapia (GIFT) (susceptible and resistant GIFT species) splenic responses to S. agalactiae infection. The results showed 1608 proteins were identified with high confidence (P < 0.05). A total of 441 and 382 DEPs were characterized in susceptible and resistant GIFT tilapia species, respectively. Gene ontology (GO) annotation showed that the DEPs covered many biological functions, including cellular process, metabolism, and immune process. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that the DEPs were mainly enriched in the lysosome, apoptosis, and complement and coagulation cascade pathways during S. agalactiae infection. By group comparisons, the proteins related to immune response, cytoskeleton, and energy metabolism of disease-resistant strains were upregulated compared with those of susceptible strain. The content determination of immune-related enzyme activity was consistent with the results obtained throughout the study. More active innate immune system and cell defense is the basis of S. agalactiae resistance for disease-resistant GIFT tilapia species. Furthermore, 34 proteins involved in disease resistance were characterized. Therefore, the present study contributes to a better understanding of the immune system and defense mechanisms of GIFT tilapia in response to S. agalactiae infection and provides valuable recommendations for breeding disease-resistant GIFT tilapia species.
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Abbreviations
- DEPs:
-
differential expression proteins
- GO:
-
gene ontology
- KEGG:
-
kyoto encyclopedia of genes and genomes
- GIFT:
-
genetically improved farmed tilapia
- SOD:
-
superoxide dismutase
- CXCL12:
-
C-X-C motif chemokine ligand 12
- MFAP4:
-
microfibril-associated glycoprotein 4
- NCCRP-1:
-
non-specific cytotoxic cell receptor protein 1
- SHP1:
-
Src homology 2 domain-containing protein tyrosine phosphatase 1
- C2CD2:
-
C2 domain-containing protein 2
- LIM3:
-
LIM domain protein-3
- EML1:
-
echinoderm microtubule-associated protein-like 1
- IFN-γ:
-
interferon-gamma
- IL-1β:
-
interleukin-1 beta
- MudPIT:
-
multidimensional protein identification technology
- iTRAQ:
-
isobaric tags for relative and absolute quantitation
- CHAPS:
-
3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate
- DTT:
-
dithiothreitol
- TEAB:
-
tetraethylammonium bromide
- FASP:
-
filter aided proteome preparation
- hp-RPLC:
-
high-pH reverse-phase liquid chromatography
- CID:
-
collision-induced dissociation
- SD:
-
standard deviation
- PSMs:
-
peptide spectrum matches
- CK:
-
control
- LZM:
-
lysozymes
- T-AOC:
-
total antioxidant capacity
- PRRs:
-
pathogen recognition receptors
- PAMPs:
-
pathogen-associated molecular patterns
- TLRs:
-
Toll-like receptors
- MHC:
-
major histocompatibility complex
- ISAV:
-
infectious salmon anemia virus
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Acknowledgements
We would like to thank the Core Facility Center of SKLCUSA for mass spectrometry support.
Funding
This study was supported by the National Key R&D Program of China (2018YFD0900302-5), Guangxi Nature Fund program (2018JJA130220), and Major Science and Technology projects in Guangxi (AA17204080-2).
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Table S1
A complete proteome list with protein names and accession numbers in disease-resistant and susceptible GIFT strains. (XLSX 821 kb)
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Zhu, J., Yu, K., Ao, Q. et al. Comparative splenic proteomic analysis of susceptible and resistant GIFT tilapia following challenge with Streptococcus agalactiae. Aquacult Int 29, 1141–1159 (2021). https://doi.org/10.1007/s10499-021-00683-9
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DOI: https://doi.org/10.1007/s10499-021-00683-9