Skip to main content
SpringerLink
Log in

Phase shifted-lateral shearing digital holographic microscopy imaging for early diagnosis of cysts in soft tissue-mimicking phantom

  • Regular Paper
  • Published:
Applied Physics B Aims and scope Submit manuscript

Abstract

The diagnosis of cyst structures that can be seen in almost every part of the body can be made using various medical imaging methods recently. Although the used methods and devices provide convenience in diagnosis and treatment, sometimes they can not detect early-stage cyst structures without showing any symptoms. In this study, the cystic structures with different size and formation in phantoms which are soft tissue-mimicking structures created without the need for any living tissue obtained with biopsy process are visualized by the proposed Phase Shifted-Lateral Shearing Digital Holographic Microscopy in three dimensional. The proposed method is verified with experimental results obtained by using various phantom models created at different times and structures. Experimental results illustrate the applicability of the proposed method for imaging of the cyst structures in the created soft tissue-mimicking phantoms even if they are in different structures, formations and stages (especially in early stage). In other respects, it enables to image the biological specimens in small size using simple and low-cost setup.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10

Similar content being viewed by others

References

  1. H. Kasban, M.A.M. El-Bendary, D.H. Salama, A comparative study of medical imaging techniques. Int. J. Inf. Sci. Intell. Syst. (IJSR) 4(2), 37–58 (2015)

    Google Scholar 

  2. J.Z. Cheng et al., Computer-aided diagnosis with deep learning architecture: applications to breast lesions in US images and pulmonary nodules in CT scans. Sci. Rep. 6(1), 1–13 (2016)

    Article  Google Scholar 

  3. Y. Lee, Improved total-variation noise-reduction technique with gradient method using iteration counter and its application in medical diagnostic chest and abdominal X-ray imaging. Optik 170(1), 475–483 (2018)

    Article  ADS  Google Scholar 

  4. E.K. Wang et al., A deep learning based medical image segmentation technique in Internet-of-Medical-Things domain. Futur. Gener. Comp. Syst. 108, 135–144 (2020)

    Article  Google Scholar 

  5. J.P. Angelo et al., Review of structured light in diffuse optical imaging. J. Biomed. Opt. 24(7), 071602 (2019)

    ADS  Google Scholar 

  6. X. Dang et al., Deep-tissue optical imaging of near cellular-sized features. Sci. Rep. 9(1), 1–12 (2019)

    ADS  Google Scholar 

  7. P. Farzam et al., Shedding light on the neonatal brain: probing cerebral hemodynamics by diffuse optical spectroscopic methods. Sci. Rep. 7(1), 1–10 (2017)

    Article  Google Scholar 

  8. N. Honda et al., Determination of optical properties of human brain tumor tissues from 350 to 1000 nm to investigate the cause of false negatives in fluorescence-guided resection with 5-aminolevulinic acid. J. Biomed. Opt. 23(7), 075006 (2018)

    Article  ADS  Google Scholar 

  9. F. Vasefi, N. MacKinnon, D.L. Farkas, B. Kateb, Review of the potential of optical technologies for cancer diagnosis in neurosurgery: a step toward intraoperative neurophotonics. Neurophotonics 4(1), 011010 (2017)

    Article  Google Scholar 

  10. B.M. Haas et al., Comparison of tomosynthesis plus digital mammography and digital mammography alone for breast cancer screening. Radiology 269(3), 694–700 (2013)

    Article  Google Scholar 

  11. I.M. Berke et al., Seeing through musculoskeletal tissues: improving in situ imaging of bone and the lacunar canalicular system through optical clearing. PLoS One 11(3), 1–29 (2016)

    Article  Google Scholar 

  12. J. Garrett, E. Fear, A new breast phantom with a durable skin layer for microwave breast imaging. IEEE Trans. Antennas Propag. 63(4), 1693–1700 (2015)

    Article  ADS  MathSciNet  MATH  Google Scholar 

  13. T.D. Vreugdenburg, C.D. Willis, L. Mundy, J.E. Hiller, A systematic review of elastography, electrical impedance scanning, and digital infrared thermography for breast cancer screening and diagnosis. Breast Cancer Res. Treat. 137(3), 665–676 (2013)

    Article  Google Scholar 

  14. Y. Xiao, Q. Zhou, Z. Chen, Automated breast volume scanning versus conventional ultrasound in breast cancer screening. Acad. Radiol. 22(3), 387–399 (2015)

    Article  Google Scholar 

  15. A. Anand, I. Moon, B. Javidi, Automated disease identification with 3-D optical imaging: a medical diagnostic tool. Proc. IEEE 105(5), 924–946 (2017)

    Article  Google Scholar 

  16. A. Anand et al., Compact, common path quantitative phase microscopic techniques for imaging cell dynamics. Pramana 82(1), 71–78 (2014)

    Article  ADS  Google Scholar 

  17. Y. Park, C. Depeursinge, G. Popescu, Quantitative phase imaging in biomedicine. Nat. Photon. 12(10), 578–589 (2018)

    Article  ADS  Google Scholar 

  18. V. Kumar, G.S. Khan., C. Shakher, Phase contrast imaging of red blood cells using digital holographic interferometric microscope, Proc. SPIE 10453 Third International Conference on Applications of Optics and Photonics, (2017) pp. 104532T

  19. V. Rastogi et al., Design and development of volume phase holographic grating based digital holographic interferometer for label free quantitative cell imaging. Appl. Opt. 59(12), 3773–3783 (2020)

    Article  ADS  Google Scholar 

  20. V.K. Lam et al., Quantitative assessment of cancer cell morphology and motility using telecentric digital holographic microscopy and machine learning. Cytom. Part A 93(3), 334–345 (2018)

    Article  Google Scholar 

  21. Z. El-Schich, A.L. Mölder, A.G. Wingren, Quantitative phase imaging for label-free analysis of cancer cells-focus on digital holographic microscopy. Appl. Sci. 8(7), 1027 (2018)

    Article  Google Scholar 

  22. P. Vora et al., Wide field of view common-path lateral-shearing digital holographic interference microscope. J. Biomed. Opt. 22(12), 126001(1–11) (2017)

    ADS  Google Scholar 

  23. A.S. Singh, A. Anand, R.A. Leitgeb, B. Javidi, Lateral shearing digital holographic imaging of small biological specimens. Opt. Express 20(21), 23617–23622 (2012)

    Article  ADS  Google Scholar 

  24. S. Devinder, A. Lal, T.R. Dastidar, S.K. Dubey, Quantitative analysis of numerically focused red blood cells using subdivided two-beam interference (STBI) based lateral-shearing digital holographic microscope, [Online]. Available: arXiv preprint arXiv:1909.03454, (2019)

  25. J. Di et al., Dual-wavelength common-path digital holographic microscopy for quantitative phase imaging based on lateral shearing interferometry. Appl. Opt. 55(26), 7287–7293 (2016)

    Article  ADS  Google Scholar 

  26. P. Marquet, C. Depeursinge, P.J. Magistretti, Review of quantitative phase-digital holographic microscopy: promising novel imaging technique to resolve neuronal network activity and identify cellular biomarkers of psychiatric disorders. Neurophotonics 1(2), 020901 (2014)

    Article  Google Scholar 

  27. Y. Cao, G.Y. Li, X. Zhang, Y.L. Liu, Tissue-mimicking materials for elastography phantoms: a review. Extreme Mech. Lett. 17(1), 62–70 (2017)

    Article  Google Scholar 

  28. V. Kumari, G. Sheoran, T. Kanumuri, SAR analysis of directive antenna on anatomically real breast phantoms for microwave holography. Microw. Opt. Techn. Lett. 62(1), 466–473 (2020)

    Article  Google Scholar 

  29. B. Karaböce, E. Çetin, M. Özdingiş, H.O. Durmuş, Image measurement verification studies of different objects in tissue-mimicking fantom, in Proc. TIPTEKNO, (2017) pp. 1-4

  30. M. Ziemczonok, A. Kuś, P. Wasylczyk, M. Kujawińska, 3D-printed biological cell phantom for testing 3D quantitative phase imaging systems. Sci. Rep. 9(1), 1–9 (2019)

    Article  Google Scholar 

  31. W.K. Lee et al., Biosynthesis of agar in red seaweeds: a review. Carbohydr. Polym. 164 (2017)

  32. M. Earle, G. De Portu, E. DeVos, Agar ultrasound phantoms for low-cost training without refrigeration. Afr. J. Emerg. Med. 6(1), 18–23 (2016)

    Article  Google Scholar 

  33. T. Tahara et al., High-speed three-dimensional microscope for based dynamically moving biological objects based on parallel phase-shift digital holographic microscopy. IEEE J. Sel. Top. Quantum Electron. 18(4), 1387–1393 (2012)

    Article  ADS  Google Scholar 

  34. T. Kreis, Handbook of Holographic Interferometry: Optical and Digital Methods, 2nd edn. (Wiley-VCH, Weinheim, 2005).

    Google Scholar 

  35. T. Fukuda et al., Three-dimensional motion-picture imaging of dynamic object by parallel-phase-shifting digital holographic microscopy using an inverted magnification optical system. Opt. Rev. 24(2), 206–211 (2017)

    Article  Google Scholar 

  36. T. Fukuda et al., Three-dimensional imaging of distribution of refractive index by parallel phase-shifting digital holography using Abel inversion. Opt. Express 25(15), 18066–18071 (2017)

    Article  ADS  Google Scholar 

  37. K. Ishikawa et al., High-speed imaging of sound using parallel phase-shifting interferometry. Opt. Express 24(12), 12922–12932 (2016)

    Article  ADS  Google Scholar 

  38. CR600x2 Product information, http://optronis.com/en/products/cr600x2-mc/

  39. CR600x2 Camera Users’ Manual, http://optronis.com/nwp-content/uploads/2017/02/Camera-manual-english-1830-SU-02-N.pdf

  40. E. Sánchez-Ortiga et al., Off-axis digital holographic microscopy: practical design parameters for operating at diffraction limit. Appl. Opt. 53(10), 2058–2066 (2014)

    Article  ADS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Electrical-Electronics Engineering, Zonguldak Bulent Ecevit University, 67100, Zonguldak, Turkey

    Tugba Ozge Onur & Gulhan Ustabas Kaya

  2. Department of Biomedical Engineering, Zonguldak Bulent Ecevit University, 67100, Zonguldak, Turkey

    Ceren Kaya

Authors
  1. Tugba Ozge Onur
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gulhan Ustabas Kaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ceren Kaya
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tugba Ozge Onur.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Onur, T.O., Ustabas Kaya, G. & Kaya, C. Phase shifted-lateral shearing digital holographic microscopy imaging for early diagnosis of cysts in soft tissue-mimicking phantom. Appl. Phys. B 127, 61 (2021). https://doi.org/10.1007/s00340-021-07607-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s00340-021-07607-8

Search

Navigation