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Simultaneous Carbon and Nitrogen Removal from Domestic Wastewater using High Rate Vermifilter

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Abstract

Being a cost-effective and environmentally benign technology, vermifiltration has significantly replaced the available conventional wastewater remediation methods in many cases over the last few decades. The present work emphasizes on the investigation of the nitrogen transformation dynamics, in addition to the organic carbon abatement in the designed high rate hybrid vermifilter. Moreover, the economical sustainability of the vermifiltration technology has also been enlightened by creating a bridge with the concept of circular bio-economy. The designed high rate macrophyte-assisted vermifilter (MAVF) ascertained significant high nitrogen and organic carbon removal efficiencies from the real domestic sewage, considering the chemical oxygen demand (COD) of the influent and hydraulic loading rate (HLR) as the input variables. The designed MAVF facilitated the maximum ammonium nitrogen (NH4+-N), organic nitrogen, and total kjeldahl nitrogen removal efficiencies up to 98.2 ± 0.70%, 100%, and 99 ± 0.47%, respectively when COD of the influent and HLR were 200 ± 25 mg/L and 3 ± 0.1 m3/m2-d, respectively. On the other hand, substantial enhancement in the nitrate nitrogen (NO3-N) in the effluent (73 ± 10.55 times its influent concentration) was observed with influent COD of 200 ± 25 mg/L and HLR of 7 ± 0.2 m3/m2-d. When the influent COD and HLR were maintained at 700 ± 45 mg/L and 3 ± 0.1 m3/m2-d, respectively, the highest total nitrogen removal of 87 ± 2.25% was obtained. Alternatively, the influent COD of 200 ± 25 mg/L and HLR of 3 ± 0.1 m3/m2-d yielded the highest COD removal efficiency of 77 ± 1.59%. Hence, the outcome of the present research work strengthens the suitability of the vermifiltration technology as an economically and ecologically sound natural wastewater bio-remediation technology for the treatment of domestic wastewater.

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References

  1. Tong T, Elimelech M (2016) The global rise of zero liquid discharge for wastewater management: drivers, technologies, and future directions. Environ Sci and Technol 50:6846–6855

    Article  CAS  Google Scholar 

  2. Verma AK, Dash RR, Bhunia P (2012) A review on chemical coagulation/flocculation technologies for removal of colour from textile wastewaters. J Environ Manage 93:154–168

    Article  CAS  PubMed  Google Scholar 

  3. Zhao L, Wang Y, Yang J, Xing M, Li X, Yi D, Dehan Deng D (2010) Earthworm microorganism interactions: a strategy to stabilize domestic wastewater sludge. Water Res 44:2572–2582

    Article  CAS  PubMed  Google Scholar 

  4. Mburu N, Tebitendwa SM, Bruggen JJAV, Rousseau DPL, Lens PNL (2013) Performance comparison and economics analysis of waste stabilization ponds and horizontal subsurface flow constructed wetlands treating domestic wastewater: a case study of the Juja sewage treatment works. J Environ Manage 128:220–225

    Article  CAS  PubMed  Google Scholar 

  5. Volkman S (2003). Sustainable wastewater treatment and reuse in urban areas of developing world. Master's thesis. Michigan Technological University

  6. Simate GS, Cluett J, Lyuke SE, Musapatika ET, Ndlovu S, Walubita LF, Alvarez AE (2011) The treatment of brewery wastewater for reuse: state of the art. Desalination 273:235–247

    Article  CAS  Google Scholar 

  7. Degrémont (1989) Water technical handbook, 9 edn. Degrémont, Paris

  8. Metcalf and Eddy (2003). Wastewater engineering, treatment and reuse, 4th edn. McGraw-Hill, New York

  9. Feng Y, Wang X, Logan BE, Lee H (2008) Brewery wastewater treatment using air cathode microbial fuel cells. Appl Microbiol Biot 78:873–880

    Article  CAS  Google Scholar 

  10. Samal K, Dash RR, Bhunia P (2017) Treatment of wastewater by vermifiltration integrated with macrophyte filter: a review. J Environ Chem Eng 5:2274–2289

    Article  CAS  Google Scholar 

  11. Singh R, Bhunia P, Dash RR (2017) A mechanistic review on vermifiltration of wastewater: design, operation and performance. J Environ Manage 197:656–672

    Article  CAS  PubMed  Google Scholar 

  12. Li YS, Xiao YQ, Qiu JP, Dai YQ, Robin P (2009) Continuous village sewage treatment by vermifiltration and activated sludge process. Water Sci Technol 60:3001–3010

    Article  CAS  PubMed  Google Scholar 

  13. Kumar T, Rajpal A, Bhargava R, Prasad KSH (2014) Performance evaluation of vermifilter at different hydraulic loading rate using riverbed material. Ecol Eng 62:77–82

    Article  Google Scholar 

  14. Nebert LD, Bloem J, Lubbers IM, van Groenigen JW (2011) Association of earthworm-denitrifier interactions with increased emission of nitrous oxide from soil mesocosms amended with crop residue. Appl Environ Microbiol 77:4097–4104

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Drake HL, Horn MA (2007) As the worm turns: the earthworm gut as a transient habitat for soil mierobial biomes. Annu Rev Mierobiol 61:169–189

    Article  CAS  Google Scholar 

  16. Horn MA, Schramm A, Drake HL (2003) The earthworm gut: an ideal habitat for ingested N2O producing microorganisms. Appl Environ Microbiol 69:1662–1669

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Burtelow AE, Bohlen PJ, Groffman PM (1998) Influence of exotic earthworm invasion on soil organic matter, microbial biomass and denitrification potential in forest soils of the northeastern United States. Appl Soil Ecol 9:197–202

    Article  Google Scholar 

  18. Costeilo DM, Lamberti GA (2009) Biological and physical effects of non-native earthworms on nitrogen eyeling hi riparian soils. Soil Biol Biochem 41:2230–2235

    Article  Google Scholar 

  19. Giannoponlos G, Pulleman MM, Van Groenigen JW (2010) Interactions between residue placement and earthworm ecological strategy affect aggregate turnover and N2O dynamics in agrieultural soil. Soil Biol Biochem 42:618–625

    Article  Google Scholar 

  20. Fierer N, Jackson JA, Viigalys R, Jackson RB (2005) Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assays. Appl Environ Microbiol 71:4117–4120

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Amador JA, Görres JH (2007) Microbiological characterization of the structures built by earthworms and ants in an agricultural field. Soil Biol Bioehem 39:2070–2077

    Article  CAS  Google Scholar 

  22. Scheu S (1987) Microbial activity and nutrient dynamics in earthworm easts (Lumbrieidae). Biol Eertil Soils 5:230–234

    Google Scholar 

  23. Parkin TB, Berry EC (1999) Microbial nitrogen transformations in earthworm burrows. Soil Biol Biochem 31:1765–1771

    Article  CAS  Google Scholar 

  24. Brown GG, Barois I, Laveile P (2000) Regulation of soil organic matter dynamics and microbial activity in the drilosphere and the role of interactions with other edaphic functional domains. Eur J Soil Biol 36:177–198

    Article  Google Scholar 

  25. Jiang L, Liu Y, Hu X, Zeng G, Wang H, Zhou L, Tan X, Huang B, Liu S, Liu S (2016) The use of microbial-earthworm ecofilters for wastewater treatment with special attention to influencing factors in performance: a review. Bioresour Technol 200:999–1007

    Article  CAS  PubMed  Google Scholar 

  26. Xing M, Li X, Yang J (2010) Treatment performance of small-scale vermifilter for domestic wastewater and its relationship to earthworm growth, reproduction and enzymatic activity. Afr J Biotechnol 9:7513–7520

    Article  CAS  Google Scholar 

  27. Singh R, Samal K, Dash RR, Bhunia P (2019) Vermifiltration as a sustainable natural treatment technology for the treatment and reuse of wastewater: a review. J Environ Manag 247:140–151

    Article  Google Scholar 

  28. Sinha RK, Heart S, Valani DB, Chauhan KA (2009) Environmental-economics of crop production by vermiculture: economically viable & environmentally sustainable over chemical agriculture. Am Euras J Agric Environ Sci 5:01–55

    Google Scholar 

  29. Kumar T, Bhargava R, Prasad KSH, Pruthi V (2015) Evaluation of vermifiltration process using natural ingredients for effective wastewater treatment. Ecol Eng 75:370–377

    Article  Google Scholar 

  30. Rajpal A, Bhargava R, Chopra AK, Kumar T (2014) Vermistabilization and nutrient enhancement of anaerobic digestate through earthworm species Perionyx excavatus and Perionyx sansibaricus. J Mater Cycles Waste Manage 16:219–226

    Article  CAS  Google Scholar 

  31. Arora S, Kazmi AA (2015) The effect of seasonal temperature on pathogen removal efficacy of vermifilter for wastewater treatment. Water Res 74:88–99

    Article  CAS  PubMed  Google Scholar 

  32. Wang DB, Zhang ZU, Li XM, Zheng W, Ding Y, Yang B, Yang Q, Zeng T, Cao J, Yue X, Shen T, Zeng G, Deng J, He X (2010) Effects of earthworms on surface clogging characteristics of intermittent sand filters. Water Sci Technol 61:2881–2888

    Article  CAS  PubMed  Google Scholar 

  33. Kumar T, Rajpal A, Arora S, Bhargava R, Prasad KSH, Kazmi AA (2016) A comparative study on vermifiltration using epigeic earthworm Eisenia fetida and Eudrilus eugeniae. Desalin Water Treat 57:6347–6354

    Article  CAS  Google Scholar 

  34. Xu D, Li Y, Howard A (2013) Influence of earthworm Eisenia fetida on removal efficiency of N and P in vertical flow constructed wetland. Environ Sci Pollut Res 20:5922–5929

    Article  CAS  Google Scholar 

  35. APHA (American Public Health Association) (2005) Standard methods for the examination of water and wastewater, 20th edn. Amer Public Health Assoc, Amer Water Works Assoc, Water poll Control Fed, Washington, USA

  36. Tchobanoglous G, Burton FL, Stensel HD (2003) Wastewater engineering, treatment and reuse, 4th edn. McGraw-Hill, New York

    Google Scholar 

  37. Kadam AM, Oza GH, Nemade PD, Shankar HS (2008) Pathogen removal from municipal wastewater in constructed soil filter. Ecol Eng 33:37–44

    Article  Google Scholar 

  38. Agren GI (2004) The C:N:P stoichiometry of autotrophs—theory and observations. Ecol Lett 7:185–191

    Article  Google Scholar 

  39. Elser JJ, Acharya K, Kyle M, Cotner J, Makino W, Markow T, Watts T, Hobbie S, Fagan W, Schade J (2003) Growth rate–stoichiometry couplings in diverse biota. Ecol Lett 6:936–943

    Article  Google Scholar 

  40. U.S.E.P.A (1993) Nitrogen. EPA/625/R-93/010, U.S. Environmental Protection Agency, Cincinnati, OH

  41. Gonenc E, Harremoes P (1990) Nitrification in rotating-disk systems. 2. Criteria for simultaneous mineralization and nitrification. Water Res 24:499–505

    Article  CAS  Google Scholar 

  42. Okabe S, Kindaichi T, Ito T, Satoh H (2004) Analysis of size distribution and areal cell density of ammonia-oxidizing bacterial microcolonies in relation to substrate microprofiles in biofilms. Biotechnol Bioeng 85:86–95

    Article  CAS  PubMed  Google Scholar 

  43. Zhang TC, Fu YC, Bishop PL (1994) Competition in biofilms. Water Sci Technol 29:263–270

    Article  CAS  Google Scholar 

  44. Elenter D, Milferstedt K, Zhang W, Hausner M, Morgenroth E (2007) Influence of detachment on substrate removal and microbial ecology in a heterotrophic/autotrophic biofilm. Water Res 41:4657–4671

    Article  CAS  PubMed  Google Scholar 

  45. Zhu S, Chen S (2001) Effects of organic carbon on nitrification rate in fixed film biofilters. Aquacult Eng 25:1–11

    Article  Google Scholar 

  46. Ebeling JM, Timmons MB, Bisogni JJ (2006) Engineering analysis of the stoichiometry of photoautotrophic, autotrophic, and heterotrophic removal of ammonia–nitrogen in aquaculture systems. Aquaculture 257:346–358

    Article  Google Scholar 

  47. Gupta AB, Gupta SK (2001) Simultaneous carbon and nitrogen removal from high strength domestic wastewater in an aerobic RBC biofilm. Water Res 35:1714–1722

    Article  CAS  PubMed  Google Scholar 

  48. Tran NH, Urase T, Ngo HH, Hu J, Ong SL (2013) Insight into metabolic and co-metabolic activities of autotrophic and heterotrophic microorganisms in the biodegradation of emerging trace organic contaminants. Biores Technol 146:721–731

    Article  CAS  Google Scholar 

  49. Her JJ, Huang JS (1995) Influences of carbon source and C/N ratio on nitrate/nitrite denitrification and carbon breakthrough. Bioresour Technol 54:45–51

    Article  CAS  Google Scholar 

  50. Xia S, Li J, Wang R (2008) Nitrogen removal performance and microbial community structure dynamics response to carbon nitrogen ratio in a compact suspended carrier biofilm reactor. Ecol Eng 32:256–262

    Article  Google Scholar 

  51. Singh R, Bhunia P, Dash RR (2019) Impact of organic loading rate and earthworms on dissolved oxygen and vermifiltration. J Hazard Toxic Radioact Waste 23:04019001

    Article  CAS  Google Scholar 

  52. Grace MA, Healy MG, Clifford E (2016) Performance and surface clogging in intermittently loaded and slow sand filters containing novel media. J Environ Manage 180:102–110

    Article  CAS  PubMed  Google Scholar 

  53. Nivala J, Knowles P, Dotro G, Garcia J, Wallace S (2012) Clogging in subsurface-flow treatment wetlands: measurement, modeling and management. Water Res 46:1625–1640

    Article  CAS  PubMed  Google Scholar 

  54. Fang CX, Zheng Z, Luo XZ, Guo FH (2010) Effect of hydraulic load on domestic wastewater treatment and removal mechanism of phosphorus in earthworm ecofilter. Fresen Environ Bull 19:1099–1108

    CAS  Google Scholar 

  55. Singh R, Bhunia P, Dash RR (2019) Optimization of organics removal and understanding the impact of HRT on vermifiltration of brewery wastewater. Sci Total Environ 651:1283–1293

    Article  CAS  PubMed  Google Scholar 

  56. Singh R, D’Alessio M, Meneses Y, Bartelt-Hunt S, Ray C (2021) Nitrogen removal in vermifiltration: mechanisms, influencing factors, and future research needs. J Environ Manage 281:111868

    Article  CAS  PubMed  Google Scholar 

  57. Arora S, Rajpal A, Kumar T, Bhargava R, Kazmi AA (2014) Pathogen removal during wastewater treatment by vermifiltration. Environ Technol 35:2493–2499

    Article  CAS  PubMed  Google Scholar 

  58. La Mora-Orozco D, González-Acuña IJ, Saucedo-Terán RA, Flores-López HE, Rubio-Arias HO, Ochoa-Rivero JM (2018) Removing organic matter and nutrients from pig farm wastewater with a constructed wetland system. Int J Environ Res Public Health 15:1031

    Article  Google Scholar 

  59. Singh R, Alessio MD, Meneses Y, Bartelt-Hunt SL, Woodbury B, Ray C (2020) Development and performance assessment of an integrated vermifiltration based treatment system for the treatment of feedlot runoff. J Clean Prod 278:123355

    Article  Google Scholar 

  60. Sirivedhin T, Gray KA (2006) Factors affecting denitrification rates in experimental wetlands: field and laboratory studies. Ecol Eng 26:167–181

    Article  Google Scholar 

  61. Wang L, Guo F, Zheng Z, Luo X, Zhang J (2011) Enhancement of rural domestic sewage treatment performance, and assessment of microbial community diversity and structure using tower vermifiltration. Bioresour Technol 102:9462–9470

    Article  CAS  PubMed  Google Scholar 

  62. Singh R, Bhunia P, Dash RR (2018) Understanding intricacies of clogging and its alleviation by introducing earthworms in soil biofilters. Sci Total Environ 633:145–156

    Article  CAS  PubMed  Google Scholar 

  63. Ghatnekar SD, Kavian MF, Sharma SM, Ghatnekar SS, Ghatnekar GS, Ghatnekar AV (2010) Application of vermi-filter-based effluent treatment plant (pilot scale) for biomanagement of liquid effluents from the gelatine industry. Dyn Soil Dyn Plant 4:83–88

    Google Scholar 

  64. Devkota D, Dhakal SC, Dhakal D, Dhakal DD, Ojha RB (2014) Economics of Production and Marketing of Vermicompost in Chitwan, Nepal. Int J Agric Soil Sci 2:112–117

    Google Scholar 

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Acknowledgements

The authors would like to thank the Environmental Engineering department of Indian Institute of Technology Bhubaneswar (IIT Bhubaneswar), Odisha, India, for providing all the necessary facilities for carrying out the research work successfully.

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Correspondence to Puspendu Bhunia.

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Dey Chowdhury, S., Bhunia, P. Simultaneous Carbon and Nitrogen Removal from Domestic Wastewater using High Rate Vermifilter. Indian J Microbiol 61, 218–228 (2021). https://doi.org/10.1007/s12088-021-00936-4

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