Abstract
The complex chemical and structural arrangement of lignocellulosic biomass demand a pretreatment step to dismantle its coherent nature. The current study was designed to study H2SO4 impregnation before hydrothermal pretreatment (HP) of sugarcane bagasse for biogas production using response surface methodology based on defining factors: temperature (160, 180, 200 °C), time (5, 12, 19 min), and H2SO4 concentration (1, 2, 3% w/v). The H2SO4 impregnation had a paramount impact in combination with temperature and treatment time parameters. The lowest solid yield 12.3% was recorded in pretreatment run A-HSO (200 °C, 19 min, 3% H2SO4), up to 100% xylan removal was noted in four pretreatment runs (A-HSO; 200 °C, 19 min, 3% H2SO4, B-HSO; 200 °C, 19 min, 1% H2SO4, C-HSO; 200 °C, 5 min, 3% H2SO4, and O-HSO; 213.6 °C, 12 min, 2% H2SO4), maximum klason lignin increase in insoluble solid fraction (ISF) verified was 358.85% in pretreatment run O-HSO at 213.6 °C, while glucan content reduction (33.18–74.14%) was also observed in pretreatment runs A-HSO, C-HSO, and O-HSO. Results verified maximum methane recovery of 160.16 NmL (g TVS)−1 in pretreatment run I-SHO (180 °C, 12 min, 2% H2SO4). Microbial community analysis observed the predominance of Flavobacterium and Desulfosporosinus genera of Bacterial domain and Methanolinea of Archaea domain. H2SO4 impregnation before HP though not suitable for methane production from ISF could be employed in a biorefinery perspective to obtain valuables (chemicals and fuels) due to the production of organic acids rich liquid stream and lignin-rich solid fraction.
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Abbreviations
- APHA:
-
American Public Health Association
- BMP:
-
Biomethane potential
- CCD:
-
Central composite design
- COD:
-
Chemical oxygen demand
- DM:
-
Dry matter
- GAIN:
-
Global Agricultural Information Network
- HP:
-
Hydrothermal pretreatment
- HPLC:
-
High-performance liquid chromatography
- NCBI:
-
National Center for Biotechnology Information
- OTU:
-
Operational taxonomic unit
- PCR:
-
Polymerase chain reaction
- RDP:
-
Ribosomal Database Project
- SB:
-
Sugarcane bagasse
- SEM:
-
Scanning electron microscopy
- TCD:
-
Thermal conductivity detector
- TS:
-
Total solids
- TVS:
-
Total volatile solids
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This research was funded by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Ministry of Education, Brazil, under the grant ID 23699920852.
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FA, ELS, and MBAV conceived and designed the study. FA conducted the experiments and wrote the manuscript. VS helped in conducting BMP experiments. IKS helped in molecular biology experiments. MBAV, IKS, and JS proofread the manuscript. All authors provided feedback on the manuscript.
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Ahmad, F., Silva, V., Sakamoto, I.K. et al. Bioprospecting Sulfuric Acid Assisted Hydrothermal Pretreatment of Sugarcane Bagasse and Microbial Community Structure for Methane Production. Bioenerg. Res. 15, 631–649 (2022). https://doi.org/10.1007/s12155-021-10268-2
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DOI: https://doi.org/10.1007/s12155-021-10268-2