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Biocontrol mechanism of Myxococcus fulvus B25-I-3 against Phytophthora infestans and its control efficiency on potato late blight

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Abstract

Phytophthora infestans is the pathogen of potato late blight, which is one of the most serious diseases of the potato. Myxobacteria, especially Myxococcus, become a valuable biological control resource due to their preponderant abilities to produce various secondary metabolites with novel structure and remarkable biological activity. In a previous study, Myxococcus fulvus B25-I-3 with antagonistic activity against P. infestans was isolated from an environmental sample by rabbit fecal induction method. The biocontrol mechanism of M. fulvus B25-I-3 against P. infestans and its control efficiency on potato late blight were studied. The results showed that the active substances produced by strain B25-I-3 had strong inhibitory effect on the asexual reproduction and sexual reproduction of P. infestans. In addition, the active substances could reduce the content of soluble proteins and the activity of the protective enzymes (polyphenol oxidase, peroxidase, phenylalanine ammonia lyase, superoxide dismutase) in P. infestans and increase the oxidative damage and permeability of cell membrane. And the active substances could inhibit the infection of the detached potato leaves by P. infestans significantly. In conclusion, M. fulvus B25-I-3 can produce active substances against P. infestans and has potential value to develop into biological pesticides for the control of potato late blight. The completion of this work may provide basic data for the isolation and identification of active substances and the development of pesticides against potato late blight.

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Funding

This study was supported by the National Natural Science Foundation of China (No. 31370058), Inner Mongolia Autonomous Region Graduate Education Innovation Program Funding Project (No. B20171012920), Natural Science Foundation Program of Inner Mongolia, China (No.2019MS03066), and the 10th Batch of “Grassland Elite” Project of Inner Mongolia, China (No. DC2000000758).

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Authors and Affiliations

  1. College of Life Sciences, Inner Mongolia Agricultural University, Hohhot, 010010, China

    Zhi Hua Wu, Qiang Ma, Zhi Ning Sun, Hai Chen Cui & Hui Rong Liu

  2. Baotou Teachers’ College, Baotou, 014030, China

    Zhi Hua Wu

  3. The Second Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, 014040, China

    Zhi Hua Wu

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  1. Zhi Hua Wu
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  2. Qiang Ma
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  4. Hai Chen Cui
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Zhihua Wu, Qiang Ma, Zhining Sun, and Haichen Cui. The first draft of the manuscript was written by Zhihua Wu, and all the authors commented on previous versions of the manuscript. All the authors read and approved the final manuscript.

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Correspondence to Hui Rong Liu.

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Wu, Z.H., Ma, Q., Sun, Z.N. et al. Biocontrol mechanism of Myxococcus fulvus B25-I-3 against Phytophthora infestans and its control efficiency on potato late blight. Folia Microbiol 66, 555–567 (2021). https://doi.org/10.1007/s12223-021-00865-1

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