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Winter hardiness analysis of wheat-rye 5R(5A)-substituted lines in Western Siberia

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Abstract

Homoeologous group 5 chromosomes of wheat possess genes that have a significant influence on the time of earing, growth habit (Vrn), frost resistance (Fr) and winter hardiness. The Vrn-A1/Fr-A1, Vrn-B1/Fr-B1 and Vrn-D1/Fr-D1 genes are assigned to the long arm of the 5A, 5B and 5D chromosomes, respectively. In rye (Secale cereale L.), Vrn-R1/Fr-R1 genes, which determine the growth habit and cold/frost resistance, are localized on chromosome 5RL. To investigate the effect of individual chromosomes on winter hardiness, as well as their interactions with the background, wheat-rye 5R(5A)-substituted lines based on winter and spring wheats were used in this study. We created the wheat-rye 5R(5A) substitution lines based on the winter wheats cvs. ‘Filatovka’ and ‘Ulʼynovka’ and based on the spring wheats cvs. ‘Rang’ and ‘Mironovskaya krupnozernaya’ to identify the role of rye chromosome 5R in controlling the growth habit and winter hardiness. Two cultivars of rye—‘Onokhoiskaya’ (spring habit) and ‘Vietnamskaya Mestnaya’—are the source of chromosome 5R. We do not know the growth habit of rye ‘Vietnamskaya Mestnaya’. Field experiments were carried out over three years during the winters of 2015/2016, 2016/2017 and 2017/2018 in the Novosibirsk Region. It was found that 5R(5A) substitution lines for spring cvs. ‘Rang’ and ‘Mironovskaya krupnozernaya’, with the participation of the spring rye ‘Onokhoiskaya’, have a winter habit. The wheat-rye 5R(5A) lines for winter cvs. ‘Ul'yanovka’ and ‘Filatovka’, with the participation of rye ‘Vietnamskaya Mestnaya’, have a facultative habit. These substituted lines can grow in both spring and autumn sowing. The evaluation of winter hardiness conducted was carried out by means of visual scoring on a 1–9 score basis. All wheat-rye-substituted lines survived in the conditions of the Novosibirsk Region. Winter hardiness scores ranged from 5 to 6 after the 2015/2016 and 2016/2017 winters, whereas winter hardiness scores varied from 8 to 9 after the 2017/2018 winter.

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References

  • Adonina IG, Leonova IN, Badaeva ED, Salina EA (2016) Genotyping of hexaploid wheat varieties from different Russian regions. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov J Genet Breed 20(1):44–50. https://doi.org/10.18699/VJ16.107

    Article  Google Scholar 

  • Barashkova EA, Vinogradova VV (1988) Assessment of winter and frost resistance of field crops. In: Udovenko GV (ed) Diagnostics of plant resistance to stress: methodical guidance. VIR, Leningrad, pp 128–154 ((in Russ))

    Google Scholar 

  • Chumanova EV, Efremova TT, Trubacheeva NV, Arbuzova VS, Rosseeva LP (2014) Chromosome composition of wheat–rye lines and the influence of rye chromosomes on disease resistance and agronomic traits. Russ J Genet 50:1169–1178. https://doi.org/10.1134/S1022795414110039

    Article  CAS  Google Scholar 

  • Dhillon T, Pearce SP, Stockinger EJ, Distelfeld A, Li C, Knox AK, Vashegyi I, Vágújfalvi A, Galiba G, Dubcovsky J (2010) Regulation of freezing tolerance and flowering in temperate cereals: the VRN-1 connection. Plant Physiol 153:1846–1858. https://doi.org/10.1104/pp.110.159079

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Farkas A, Molnár I, Kiss T, Karsai I, Molnár-Láng M (2014) Effect of added barley chromosomes on the flowering time of new wheat/winter barley addition lines in various environments. Euphytica 195:45–55. https://doi.org/10.1007/s10681-013-0970-7

    Article  Google Scholar 

  • Edwards K, Johnstone C, Thompson C (1991) A simple and rapid method for the preparation of plant genomic DNA for PCR Analysis. Nucl Acids Res 19:1349

    Article  CAS  Google Scholar 

  • Efremova TT, Maystrenko OI, Arbuzova VS, Laikova LI, Panina GM, Popova OM, Berezova OV (2006) Effect of alien 5R(5A) chromosome substitution on ear-emergence time and winter hardiness in wheat-rye substitution lines. Euphytica 151:145–153. https://doi.org/10.1007/s10681-006-9135-2

    Article  CAS  Google Scholar 

  • Fowler DB, Dvorak J, Gusta LV (1977) Comparative cold hardiness of several Triticum species and Secale cereale L. Crop Sci 17:941–943

    Article  Google Scholar 

  • Erath W, Bauer E, Fowler DB, Gordillo A, Korzun V, Ponomareva M, Schmidt M, Schmiedchen B, Wilde P, Schön C-C (2017) Exploring new alleles for frost tolerance in winter rye. Theor Appl Genet 130:2151–2164. https://doi.org/10.1007/s00122-017-2948-7

    Article  CAS  PubMed  Google Scholar 

  • Fowler DB, Limin AE, Ritchie JT (1999) Low-temperature tolerance in cereals: model and genetic interpretation. Crop Sci 39:626–633

    Article  Google Scholar 

  • Fowler DB, Limin AE (2004) Interactions among factors regulating phenological development and acclimation rate determine low-temperature tolerance in wheat. Ann Bot 94:717–724. https://doi.org/10.1093/aob/mch196

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Fu D, Szűcs P, Yan L, Helguera M, Skinner JS, Von Zitzewitz J, Hayes PM, Dubcovsky J (2005) Large deletions within the first intron in VRN-1 are associated with spring growth habit in barley and wheat. Mol Gen Genom 273:54–65. https://doi.org/10.1007/s00438-004-1095-4

    Article  CAS  Google Scholar 

  • Galiba G, Quarrie SA, Sutka J, Morgounov A, Snape JW (1995) RFLP mapping of the vernalization (Vrn1) and frost resistance (Fr1) genes on chromosome 5A of wheat. Theor Appl Genet 90:1174–1179

    Article  CAS  Google Scholar 

  • Galiba G, Vagujfalvi A, Li C, Soltesz A, Dubcovsky J (2009) Regulatory genes involved in the determination of frost tolerance in temperate cereal. Plant Sci 176:12–19. https://doi.org/10.1016/j.plantsci.2008.09.016

    Article  CAS  Google Scholar 

  • Gray GR, Chauvin LP, Sarhan F, Huner N (1997) Cold acclimation and freezing tolerance (a complex interaction of light and temperature). Plant Physiol 114:467–474

    Article  CAS  Google Scholar 

  • Gusta LV, Fowler DB (1979) Cold resistance and injury in winter cereals. In: Mussel H, Staples RC (eds) Stress physiology in crop plants. Wiley, New York, pp 160–178

    Google Scholar 

  • Hyles J, Bloomfield MT, Hunt JR, Trethowan RM, Trevaskis B (2020) Phenology and related traits for wheat adaptation. Heredity 125:417–430. https://doi.org/10.1038/s41437-020-0320-1

    Article  PubMed  PubMed Central  Google Scholar 

  • Korzun V, Melz G, Börner A (1996) RFLP mapping of the dwarfing (Ddw1) and hairy peduncle (Hp) genes on chromosome 5 of rye (Secale cereale L.). Theor Appl Genet 92:1073–1077

    Article  CAS  Google Scholar 

  • Kozlov VE (2012) Comparison of methods for obtaining genetic diversity for breeding winter-hardy wheat in Siberia. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov J Genet Breed 16(1):232–239 ((in Russ))

    Google Scholar 

  • Kozlov VE, Ponomarenko VI, Razmakhnin EP (2018) Seasonal life history plasticity of the group of winter wheat and triticale accessions. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov J Genet Breed 22(3):310–315. https://doi.org/10.18699/VJ18.365 ((in Russ))

    Article  Google Scholar 

  • Kuperman FM, Rzhanova EI, Murashev VV, Lvova IN, Akhundova VA, Sedova IN, Shcherbina IP (1982) Development biology of cultivated plants. Graduate School, Moscow, 341p (in Russ)

  • Law CN, Worland AJ, Giorgi B (1976) The genetic control of ear emergence by chromosomes 5A and 5D of wheat. Heredity 36:49–58

    Article  Google Scholar 

  • Law CN, Worland AJ (1997) Genetic analysis of some flowering time and adaptive traits wheat. New Phytol 137:19–28

    Article  Google Scholar 

  • Leach RC, Dundas IS (2006) Single nucleotide polymorphic marker enabling rapid and early screening for the homoeolocus of β-amylase-R1: a gene linked to copper efficiency on 5RL. Theor Appl Genet 113:301–307. https://doi.org/10.1007/s00122-006-0296-0

    Article  CAS  PubMed  Google Scholar 

  • Limin AE, Fowler DB (2006) Low-temperature tolerance and genetic potential in wheat (Triticum aestivum L.): response to photoperiod, vernalization, and plant development. Planta 224:360–366. https://doi.org/10.1007/s00425-006-0219-y

    Article  CAS  PubMed  Google Scholar 

  • Mahfoozi S, Limin AE, Fowler DB (2001) Developmental regulation of low-temperature tolerance in winter wheat. Ann Bot 87:751–757. https://doi.org/10.1006/anbo.2001.1403

    Article  Google Scholar 

  • McIntosh RA, Yamazaki Y, Dubcovsky J, Rogers WJ, Morris C, Appels R, Xia XC (2013) Catalogue of gene symbols for wheat. http:/wheat/komugi/genes

  • Milec Z, Tomková L, Sumíková T, Pánková K (2012) A new multiplex PCR test for the determination of Vrn-B1 alleles in bread wheat (Triticum aestivum L.). Mol Breed 30:317–323. https://doi.org/10.1007/s11032-011-9621-7

    Article  CAS  Google Scholar 

  • Morgounov AI, Alborran M, Rajaram S (1998) Selecting winter/facultative wheat genotypes from spring × spring crosses. Euphytica 100:395–400

    Article  Google Scholar 

  • Muñoz-Amatriaín M, Hernandez J, Herb D, Baenziger PS, Bochard AM, Capettini F, Casas A, Cuesta-Marcos A, Einfeldt C, Fisk S, Genty A, Helgerson L, Herz M, Hu G, Igartua E, Karsai I, Nakamura T, Sato K, Smith K, Stockinger E, Thomas W, Hayes P (2020) Perspectives on low temperature tolerance and vernalization sensitivity in barley: prospects for facultative growth habit. Front Plant Sci 11:585927. https://doi.org/10.3389/fpls.2020.585927

    Article  PubMed  PubMed Central  Google Scholar 

  • Plaschke I, Börner A, Xie BX, Koebner RM, Schlege R, Gale MD (1993) RFLP mapping of genes affecting plant height and growth habit in rye. Theor Appl Genet 85:1049–1054

    Article  CAS  Google Scholar 

  • Pugsley A (1983) The impact of plant physiology on Australian wheat breeding. Euphytica 32:743–748

    Article  Google Scholar 

  • Rigin BV (2012) Spring type of common wheat (Triticum aestivum L.) development: phenological and genetical aspects. Proc Appl Bot Genet Breed 170:17–34 ((in Russ))

    Google Scholar 

  • Roberts DWA (1990) Identification of loci on chromosome-5A of wheat involved in control of cold hardiness, vernalization, leaf length, rosette growth habit, and height of hardened plants. Genome 33:247–259

    Article  Google Scholar 

  • Silkova OG, Dobrovolskaya OB, Adonina IG, Kravtsova LA, Salina EA, Shchapova AI, Shumny VK, Dubovets NI, Roeder MS (2006) Production of wheat-rye substitution lines and identification of chromosome composition of karyotypes using C-banding, GISH, and SSR markers. Russ J Genet 42:645–653. https://doi.org/10.1134/S1022795406060093

    Article  CAS  Google Scholar 

  • Silkova OG, Dobrovolskaya OB, Dubovets NI, Adonina IG, Kravtsova LA, Shchapova AI, Shumny VK (2007) Production of wheat–rye substitution lines based on winter rye cultivars with karyotype identification by means of C-banding, GISH, and SSR Markers. Russ J Genet 43:957–960. https://doi.org/10.1134/S1022795407080200

    Article  CAS  Google Scholar 

  • Snape JW, Law CN, Parker BB, Worland AJ (1985) Genetical analysis of chromosome 5A of wheat and its influence on important agronomic characters. Theor Appl Genet 71:518–526

    Article  CAS  Google Scholar 

  • Snape JW, Sarma R, Quarrie SA, Fish L, Galiba G, Sutka J (2001) Mapping genes for flowering time and frost tolerance in cereals using precise genetic stocks. Euphytica 120:309–315. https://doi.org/10.1023/A:1017541505152

    Article  CAS  Google Scholar 

  • Stockinger EJ, Skinner JS, Gardner KG, Francia E, Pecchioni N (2007) Expression levels of barley Cbf genes at the Frost resistance-H2 locus are dependent upon alleles at Fr-H1 and Fr-H2. Plant J 51:308–321. https://doi.org/10.1111/j.1365-313X.2007.0141.x

    Article  CAS  PubMed  Google Scholar 

  • Sutka J, Galiba G, Vagujfalvi A, Gill BS, Snape JW (1999) Physical mapping of the Vrn-A1 and Fr1 genes on chromosome 5A of wheat using deletion lines. Theor Appl Genet 99:199–202

    Article  CAS  Google Scholar 

  • Sutka J (2001) Genes for frost resistance in wheat. Euphytica 119:167–172. https://doi.org/10.1023/A:1017520720183

    Article  CAS  Google Scholar 

  • Tchebakova NM, Parfenova EI, Lysanova GI, Soja AJ (2011) Agroclimatic potential across central Siberia in an altered twenty-first century. Environ Res Lett 6:045207. https://doi.org/10.1088/1748-9326/6/4/045207

    Article  Google Scholar 

  • Tóth B, Galiba G, Feher E, Sutka J, Snape JW (2003) Mapping genes affecting flowering time and frost resistance on chromosome 5B of wheat. Theor Appl Genet 107:509–514. https://doi.org/10.1007/s00122-003-1275-3

    Article  CAS  PubMed  Google Scholar 

  • Vágújfalvi A, Galiba G, Cattivelli L, Dubcovsky J (2003) The cold-regulated transcriptional activator Cbf3 is linked to the frost tolerance locus Fr-A2 on wheat chromosome 5A. Mol Gen Genom 269:60–67. https://doi.org/10.1007/s00438-003-0806-6

    Article  CAS  Google Scholar 

  • Vágújfalvi A, Aprile A, Miller A, Dubcovsky J, Delugu G, Galiba G, Cattivelli L (2005) The expression of several CBF genes at the Fr-A2 locus is linked to frost resistance in wheat. Mol Gen Genom 274:506–514. https://doi.org/10.1007/s00438-005-0047-y

    Article  CAS  Google Scholar 

  • Yan L, Helguera M, Kato K, Fukuyama S, Sherman J, Dubcovsky J (2004) Allelic variation at the VRN-1 promoter region in polyploid wheat. Theor Appl Genet 109:1677–1686. https://doi.org/10.1007/s00122-004-1796-4

    Article  CAS  PubMed  Google Scholar 

  • Yamamoto M, Mukai Y (2005) High-resolution physical mapping of the secalin-1 locus of rye on extended DNA fibers. Cytogenet Genome Res 109:79–82. https://doi.org/10.1159/000082385

    Article  CAS  PubMed  Google Scholar 

  • Zhao Y, Gowda M, Würschum T, Longin CFH, Korzun V, Kollers S, Schachschneider R, Zeng J, Fernando R, Dubcovsky J, Reif JC (2013) Dissecting the genetic architecture of frost tolerance in Central European winter wheat. J Exp Bot 64:4453–4460. https://doi.org/10.1093/jxb/ert259

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhang J, Wang Y, Wu S, Yang J, Liu H, Zhou Y (2012) A single nucleotide polymorphism at the Vrn-D1 promoter region in common wheat is associated with vernalization response. Theor Appl Genet 125:1697–1704. https://doi.org/10.1007/s00122-012-1946-z

    Article  PubMed  Google Scholar 

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Acknowledgements

This research was supported by the budgetary project 0259-2021-0018.

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Authors and Affiliations

  1. The Federal Research Center Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    T. T. Efremova, E. V. Chumanova & I. M. Zhukova

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  1. T. T. Efremova
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  2. E. V. Chumanova
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Correspondence to T. T. Efremova.

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Communicated by M. Molnár-Láng.

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Efremova, T.T., Chumanova, E.V. & Zhukova, I.M. Winter hardiness analysis of wheat-rye 5R(5A)-substituted lines in Western Siberia. CEREAL RESEARCH COMMUNICATIONS 50, 25–35 (2022). https://doi.org/10.1007/s42976-021-00147-z

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