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The first report on the occurrence race 9 of the tomato leaf mold pathogen Cladosporium fulvum (syn. Passalora fulva) in Cuba

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Abstract

Leaf mold caused by the fungus Cladosporium fulvum (syn. Passalora fulva) has been a serious disease in greenhouse tomatoes in Cuba, but less damage is seen in outdoor grown plants. This study was conducted to identify races of the causal agent of tomato leaf mold. A total of 36 single conidial isolates obtained from different provinces in Cuba and the reference strain race 0 were inoculated on a differential set of tomato cultivars carrying different Cf resistance genes. Further, a polymerase chain reaction (PCR) was developed using Avr9 gene-specific primers. As result, all isolates could overcome the Cf-9 resistance gene only, but not resistance genes Cf-2, Cf-4 and Cf-5 and must therefore be coined race 9. Race identity was also confirmed by PCR analysis, which showed that the Avr9 gene was not amplified in any of 36 single conidial isolates studied proving that they all lack this gene. This is the first report of the occurrence of race 9 of C. fulvum in Cuba. Pyramiding other Cf genes in hybrid of tomato may provide a more durable level of resistance to C. fulvum.

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Acknowledgements

We thank Prof. PhD. Monica Höfte (Ghent University) for providing seeds of cultivar Moneymaker NILs and Prof. PhD. Bart Thomma (Wageningen University), Luis Rojas and PhD. María I. Oloriz (Instituto de Biotecnología de las Plantas) for supplying laboratory facilities. This research was performed in the frame of the Institutional University Cooperation Programme with Universidad Central “Marta Abreu” de Las Villas, supported by the Flemish Interuniversity Council (IUC UCLV/VLIR).

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Authors and Affiliations

  1. Laboratorio de Fitopatología, Departamento de Agronomía, Facultad de Ciencias Agropecuarias, Universidad Central “Marta Abreu” de Las Villas, Road to Camajuaní km 5.5, 54 830, Santa Clara, Cuba

    Alexander Bernal-Cabrera & Lidcay Herrera-Isla

  2. Department of Plant Protection, Laboratory of Mycology, National Animal and Plant Health Center, Street to Tapaste and National Highway km 22.5, Post office box 10, San José de las Lajas, Havana, Cuba

    Benedicto Martínez-Coca, Danay Ynfante-Martínez & Belkis Peteira-Delgado

  3. Departamento de Biología, Facultad de Ciencias Agropecuarias, Universidad Central “Marta Abreu” de Las Villas, Road to Camajuaní km. 5.5, 54 830, Santa Clara, Cuba

    Orelvis Portal

  4. Centro de Investigaciones Agropecuarias, Facultad de Ciencias Agropecuarias, Universidad Central “Marta Abreu” de Las Villas, Road to Camajuaní km. 5.5, 54 830, Santa Clara, Cuba

    Orelvis Portal

  5. Laboratorio de Biotecnología, Facultad de Ciencias Agropecuarias, Universidad Técnica de Ambato, Cantón Cevallos vía a Quero, sector el Tambo-la Universidad, Cevallos, 1801334, Ambato, Tungurahua, Ecuador

    Michel Leiva-Mora

  6. Laboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands

    Pierre J. G. M. de Wit

Authors
  1. Alexander Bernal-Cabrera
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  2. Benedicto Martínez-Coca
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  3. Lidcay Herrera-Isla
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  4. Danay Ynfante-Martínez
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  5. Belkis Peteira-Delgado
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  6. Orelvis Portal
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  7. Michel Leiva-Mora
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  8. Pierre J. G. M. de Wit
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Corresponding author

Correspondence to Alexander Bernal-Cabrera.

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Bernal-Cabrera, A., Martínez-Coca, B., Herrera-Isla, L. et al. The first report on the occurrence race 9 of the tomato leaf mold pathogen Cladosporium fulvum (syn. Passalora fulva) in Cuba. Eur J Plant Pathol 160, 731–736 (2021). https://doi.org/10.1007/s10658-021-02261-4

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  • DOI: https://doi.org/10.1007/s10658-021-02261-4

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