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Initiation of Nyssomyia neivai and Migonemyia migonei (Diptera: Psychodidae: Phlebotominae) colonies under laboratory conditions from wild blood fed females in northwest Argentina

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Abstract

Sand flies (Diptera: Psychodidae: Phlebotominae) are the vectors of Leishmania parasites, which are the causal agents of leishmaniasis. In this work, the survival and developmental time of the immature sand fly stages of Nyssomyia neivai and Migonemyia migonei were studied under laboratory conditions for one generation. Sand flies were collected in a rural dwelling of San Ramón de la Nueva Orán, Salta, Argentina and conditioned in individual rearing containers at 25±2 °C and 80–90% humidity. A total of 38 females of Ny. neivai oviposited 1,392 eggs (\(\overline{\mathrm x}\) = 40.81±16.5) in 5.6 days whereas two females of Mg. migonei oviposited 119 eggs (\(\overline{\mathrm x}\) = 59.50±4.95) in seven days. Migonemyia migonei presented a higher hatching percentage (66.39%) than Ny. neivai (60.99%), without statistical differences between the species (p > 0.05). A total of 78 adults of Ny. neivai emerged (5.60%) while 27 adults (22.69%) emerged for Mg. migonei (p < 0.0001). The survival curves of both species showed that the mortality decreased in the last stages and tended to constant values. This is the first study about the life cycle of sand flies present in northern part of province of Salta, the most endemic area of tegumentary leishmaniasis in Argentina. These results would allow the sustained study of sand flies in the laboratory to deepen the knowledge and understanding of their biology. Finally, it would contribute to elucidate the epidemiological role of the most abundant sand fly species in our study area where Leishmania (Viannia) braziliensis prevails.

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References

  • Andrade Filho JD, Galati EA, Falcão AL (2004) Biology of the first generation of a laboratory colony of Nyssomyia intermedia (Lutz & Neiva, 1912) and Nyssomyia neivai (Pinto, 1926) (Diptera: Psychodidae). Mem Inst Oswaldo Cruz 99(6):597–601

    Article  Google Scholar 

  • Barreto MP (1942) Contribuição para o estudo da biologia dos flebótomos em condições experimentais (Diptera Psychodidae). Med. USP, São Paulo, Brasil, Tese de Doutorado, Fac

    Google Scholar 

  • Begon M, Townsend CR, Harper JL (2006) Life, Death and Life Histories. Ecology: from individuals to ecosystems. pp. 89–131. Blackwell Publishing Ltd, England

  • Cárdenas E, Ferro C, Corredor D, Martínez O, Munstermann LE (1999) Reproductive biology of Lutzomyia shannoni (Dyar) (Diptera: Psychodidae) under experimental conditions. J Vector Ecol 24(2):158–170

    PubMed  Google Scholar 

  • Casanova C (2001) Avaliação do potencial biológico de Lutzomyia intermedia s.l. como vetor da leishmaniose tegumentar americana na zona rural do município de Conchal, estado de São Paulo. Tesis Doctoral, Faculdade de Saúde Pública, Universidad de São Paulo, São Paulo

  • Copa GN, Almazán MC, Aramayo LV, Krolewiecki AJ, Cajal SP, Juarez M, Lauthier JJ, Korenaga M, Barroso P, Nasser JR, Marco JD, Gil JF (2019) Tegumentary leishmaniasis and sand flies in a border area between Argentina and Bolivia. Trans R Soc Trop Med Hyg 113(2):91–100

    Article  Google Scholar 

  • Córdoba-Lanús E, De Grosso ML, Piñero JE, Valladares B, Salomón OD (2006) Natural infection of Lutzomyia neivai with Leishmania spp. in northwestern Argentina. Acta Trop 98(1):1–5

  • De Almeida DN, Da Silva OR, Brazil BG, Soares MJ (2004) Patterns of exochorion ornaments on eggs of seven South American species of Lutzomyia sand flies (Diptera: Psychodidae). J Med Entomol 41(5):819–825

    Article  Google Scholar 

  • Di Rienzo JA, Casanoves F, Balzarini MG, Gonzalez L, Tablada M, Robledo CW. InfoStat versión 2016. Grupo InfoStat, FCA, Universidad Nacional de Córdoba, Argentina. http://www.infostat.com.ar

  • Escovar J, Bello FJ, Morales A, Moncada L, Cárdenas E (2004) Life tables and reproductive parameters of Lutzomyia spinicrassa (Diptera: Psychodidae) under laboratory conditions. Mem Inst Oswaldo Cruz 99(6):603–607

    Article  Google Scholar 

  • Feliciangeli MD (2004) Natural breeding places of phlebotomine sandflies. Med Vet Entomol Mar 18(1):71–80

  • Galati EA (2003) Phlebotominae (Diptera, Psychodidae): Morphology, adult terminology and identification of the taxa of America. In Rangel EF, Lainson R, Editors Ed Fiocruz, Rio de Janeiro pp. 53–175

  • Goulart TM, da Rocha Silva FB, Machado VE, Oliveira WHC, de Castro CF, Rodrigues MVN, Pinto MC (2017) Breeding protocol for the sand fly Nyssomyia neivai (Diptera: Psychodidae) in laboratory conditions. Acta Trop 174:102–105

    Article  Google Scholar 

  • Goulart TM, de Castro CF, Machado VE, da Rocha Silva FB, Pinto MC (2015) Techniques to improve the maintenance of a laboratory colony of Nyssomyia neivai (Diptera: Psychodidae). Parasit Vectors 8:423

    Article  Google Scholar 

  • Hashiguchi Y, Gomez EA, Velez LN, Villegas NV, Kubo M, Mimori T, Hashiguchi K, Kato H (2020) Anthropophilic phlebotomine sand fly Lutzomyia species and search for the natural Leishmania infections in an area endemic for cutaneous leishmaniasis in Ecuador. Acta Trop 203:105287

    Article  Google Scholar 

  • Hernández D, Rojas E, Scorza JV, Jorquera A (2006) [Dog (Canis familiaris) infectivity to Lutzomyia youngi in Trujillo, Venezuela]. Biomedica 242–248

  • Hertig M (1964) Laboratory colonization of Central American Phlebotomus sandflies. Bull World Health Organ 31:569–570

    CAS  PubMed  PubMed Central  Google Scholar 

  • Killick-Kendrick R (1999) The biology and control of Phlebotomine sand flies. Clin Dermatol 17:279–289

    Article  CAS  Google Scholar 

  • Killick-Kendrick R, Leaney AJ, Ready PD (1977) The establishment, maintenance and productivity of a laboratory colony of Lutzomyia longipalpis (Diptera:Psychodidae). J Med Entomol 13(4–5):429–440

    Article  CAS  Google Scholar 

  • Krolewiecki AJ, Gil JF, Quipildor M, Cajal SP, Pravia C, Juarez M, Villalpando C, Locatelli FM, Chanampa M, Castillo G, Oreste MF, Hoyos CL, Negri V, Nasser JR (2013) Restricted outbreak of American tegumentary leishmaniasis with high microfocal transmission. Am J Trop Med Hyg 88(3):578–582

    Article  CAS  Google Scholar 

  • Lawyer P, Killick-Kendrick M, Rowland T, Rowton E, Volf P (2017) Laboratory colonization and mass rearing of phlebotomine sand flies (Diptera, Psychodidae). Parasite 24:42

    Article  Google Scholar 

  • Marialva EF, Secundino NF, Fernandes FF, Araújo HRC, Ríos-Velásquez CM, Pimenta PFP, Pessoa FAC (2020) Morphological aspects of immature stages of Migonemyia migonei (Diptera: Psychodidae, Phlebotominae), an important vector of Leishmaniosis in South America, described by scanning electron microscopy. PLoS One 12;15(11):e0242163

  • ES Marquez EA Castro de LB Nabut MCV Costa-Ribeiro da DCT Araújo L, Poubel SB, Gonçalves AL, Cruz MFR, Dos Santos Trad APME, Dias RAF, Navarro IT, Thomaz-Soccol V, (2017) Cutaneous leishmaniosis in naturally infected dogs in Paraná, Brazil, and the epidemiological implications of Leishmania (Viannia) braziliensis detection in internal organs and intact skin. Vet Parasitol 243:219–225

  • Nieves E, Ribeiro A, Brazil R (1997) Physical factors influencing the oviposition of Lutzomyia migonei (Diptera: Psychodidae) in laboratory conditions. Mem Inst Oswaldo Cruz 92(6):733–737

    Article  CAS  Google Scholar 

  • Rangel EF, de Souza NA, Wermelinger ED, Barbosa AF (1985) Establishment of a colony, in laboratory, of Lutzomyia intermedia Lutz & Neiva, 1912 (Diptera, Psychodidae, Phlebotominae). Mem Inst Oswaldo Cruz 80(2):219–226

    Article  CAS  Google Scholar 

  • Ready PD (2013) Biology of phlebotomine sand flies as vectors of disease agents. Annu Rev Entomol 58:227–250

    Article  CAS  Google Scholar 

  • Rocha DA, Costa LMD, Pessoa GDC, Obara MT (2021) Methods for detecting insecticide resistance in sand flies: A systematic review. Acta Trop 213:105747

    Article  CAS  Google Scholar 

  • Rosa J, Pereira DP, Brazil RP, Filho JD, Salomón O, Szelag E (2012) Natural infection of cortelezzii complex (Diptera: Psychodidae: Phlebotominae) with Leishmania braziliensis in Chaco. Argentina Acta Trop 123(2):128–131

    Article  Google Scholar 

  • Sábio PB, Brilhante AF, Quintana MG, Andrade AJ, Galati EAB (2016) On the Synonyms of Psathyromyia (Psathyromyia) shannoni (Dyar, 1929) and Pa. bigeniculata (Floch & Abonnenc, 1941) and the Resuscitation of Pa. pifanoi (Ortiz, 1972) With the Description of Its Female (Diptera: Psychodidae: Phlebotominae). J Med Entomol 53(5):1140–1147

  • Salomon OD, Quintana M, Rosa J (2008) Eco-epidemiology of Cutaneous Leishmaniasis in Argentina. SIICSALUD 16:514–520

    Google Scholar 

  • Salomón OD, Wilson ML, Munstermann LE, Travi BL (2004) Spatial and temporal patterns of phlebotomine sand flies (Diptera: Psychodidae) in a cutaneous leishmaniasis focus in northern Argentina. J med entomol 41(1):33–39

    Article  Google Scholar 

  • Shimabukuro PHF, de Andrade AJ, Galati EAB (2017) Checklist of American sand flies (Diptera, Psychodidae, Phlebotominae): genera, species, and their distribution. Zookeys. 8(660):67–106

  • Souza NA, Andrade-Coelho CA, Barbosa AF, Vilela ML, Rangel EF, Deane MP (1995) The influence of sugars and amino acids on the blood-feeding behaviour, oviposition and longevity of laboratory colony of Lutzomyia longipalpis (Lutz & Neiva, 1912) (Diptera: Psychodidae, Phlebotominae). Mem Inst Oswaldo Cruz 90(6):751–757

    Article  CAS  Google Scholar 

  • Souza NA, Andrade-Coelho CA, Vilela ML, Barbosa AF, Rangel EF (1999) A new larval diet for colonization of Phlebotominae sand flies. Mem Inst Oswaldo Cruz 94(6):845–847

    Article  CAS  Google Scholar 

  • Tiwary P, Singh SK, Kushwaha AK, Rowton E, Sacks D, Singh OP, Sundar S, Lawyer P (2017) Establishing, Expanding, and Certifying a Closed Colony of Phlebotomus argentipes (Diptera: Psychodidae) for Xenodiagnostic Studies at the Kala Azar Medical Research Center, Muzaffarpur, Bihar. India J Med Entomol 54(5):1129–1139

    Article  CAS  Google Scholar 

  • Truppel JH, Otomura F, Teodoro U, Massafera R, da Costa-Ribeiro MC, Catarino CM, Dalagrana L, Costa Ferreira ME, Thomaz-Soccol V (2014) Can equids be a reservoir of Leishmania braziliensis in endemic areas? PLoS One 9(4):e93731

    Article  Google Scholar 

  • Volf P, Volfova V (2011) Establishment and maintenance of sand fly colonies. J Vector Ecol 36(Suppl 1):S1–S9

    Article  Google Scholar 

  • Young DG, Duncan MA (1994) Guide to the identification and geographic distribution of Lutzomyia sand flies in Mexico, the West Indies, Central and South America (Diptera: Psychodidae). Mem Am Entomol Inst 54:1–881

Download references

Acknowledgments

We express our sincerest thanks the staff of Instituto de Investigaciones de Enfermedades Tropicales UNSa – Sede Regional Orán for collaboration in the collection and processing of samples: Tech. Gabriela Flores and Tech. Andrés Escala. We thank Dr. Alejandro Krolewiecki for providing part of the equipment to carry out this study.

Funding

This study was supported by the Consejo de Investigación de la Universidad Nacional de Salta (CIUNSa 2352, 2544, 2679) and Fondo para la Investigación Científica y Tecnológica (FONCYT)—Proyectos de Investigación Científica y Tecnológica (PICT) 2016 (4213).

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Authors and Affiliations

  1. Cátedra de Química Biológica y Biología Molecular, Facultad de Ciencias Naturales, Universidad Nacional de Salta, Universidad Nacional de Salta, Salta, Argentina

    G. N. Copa, M. C. Almazán, J. R. Nasser & J. F. Gil

  2. Instituto de Investigaciones de Enfermedades Tropicales (IIET), Facultad de Ciencias de la Salud, Sede Regional Orán, Universidad Nacional de Salta, Salta, Argentina

    G. N. Copa, M. C. Almazán & J. F. Gil

  3. Departamento de Biologia Animal, Universidade Estadual de Campinas (UNICAMP), Campinas, Brazil

    T. M. Goulart

  4. Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista (UNESP), Araraquara, Brazil

    M. C. Pinto

  5. Instituto de Patología Experimental (IPE-CONICET). Facultad de Ciencias de la Salud, Universidad Nacional de Salta, Salta, Argentina

    D. Marco

  6. Departamento de Física, Instituto de Investigación en Energía No Convencional (INENCO-CONICET), Universidad Nacional de Salta, Salta, Argentina

    J. F. Gil

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  1. G. N. Copa
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  2. M. C. Almazán
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  6. J. R. Nasser
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  7. J. F. Gil
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Contributions

GNC and JFG contributed to the study design, sand flies captured, data analysis, laboratory experiments, literature search and writing of the manuscript. MCA, TMG, MCP, DM and JN contributed to literature search and writing of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to J. F. Gil.

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Copa, G.N., Almazán, M.C., Goulart, T.M. et al. Initiation of Nyssomyia neivai and Migonemyia migonei (Diptera: Psychodidae: Phlebotominae) colonies under laboratory conditions from wild blood fed females in northwest Argentina. Int J Trop Insect Sci 41, 3065–3074 (2021). https://doi.org/10.1007/s42690-021-00500-z

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