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Composition and co-occurrence patterns of Phragmites australis rhizosphere bacterial community

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Abstract

High oxygen levels and root exudates together provide a resource-enriched habitat for rhizosphere microbes that, in turn, foster plant growth and perform key ecological functions. Plant genotype is a main factor shaping rhizosphere bacterial communities; however, the influence of plant genotype on the rhizosphere bacterial community of aquatic macrophytes remains unknown. Here we collected samples of the rhizosphere and bulk sediments of two genotypes of the macrophyte Phragmites australis from the littoral areas of freshwater lakes in China. High-throughput sequencing of the 16S rRNA gene was used to characterize the rhizosphere bacterial community. We found that the rhizosphere recruited a distinct bacterial community relative to that of the bulk sediment. The rhizosphere microbial community was characterized by distinct community composition and core OTUs comprising a few dominant taxa involved in the regulation of plant fitness and nutrient cycling. These taxa included Arthrobacter, Pseudomonas, Trichococcus, and Ramlibacter. Network analysis showed distinct co-occurrence patterns and a genotype-specific preference for hub taxa within the rhizosphere bacterial communities of each genotype. Functional analysis revealed difference between the relative abundance of functional groups participating in C, N, and S cycling. Our results improve our understanding of the composition of the rhizosphere bacterial community of aquatic macrophytes and highlight the importance of a comprehensive consideration of plant genotype in plant bioremediation in aquatic ecosystems.

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Acknowledgements

This work was financially supported by the National Key R&D Program of China (2016YFC0402710), the National Natural Science Foundation of China (32022050, 31730013, 41871096 and 31971478), the Second Tibetan Plateau Scientific Expedition and Research Program (STEP) (2019QZKK0503), the Natural Science Foundation of Jiangsu Province, China (BK20181311), and the Project of Young Scientist Group of NIGLAS (2021NIGLAS-CJH01).

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Authors and Affiliations

  1. Joint International Research Laboratory of Global Change and Water Cycle, State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering, Hohai University, Nanjing, 210098, China

    Siwen Hu, Rujia He, Wanjie Wang, Dayong Zhao, Rui Huang & Zhongbo Yu

  2. State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing, 210008, China

    Siwen Hu, Rujia He & Jin Zeng

  3. The Experimental Teaching Center of Water Resources of Hohai University, Hohai University, Nanjing, 210098, China

    Wanjie Wang

  4. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, Hubei, China

    Ming Duan

Authors
  1. Siwen Hu
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  2. Rujia He
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  3. Wanjie Wang
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  4. Dayong Zhao
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  5. Jin Zeng
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  6. Rui Huang
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  7. Ming Duan
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  8. Zhongbo Yu
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Contributions

SH contributed to data curation, writing-original draft, formal analysis, and visualization. RH helped in data curation, formal analysis, and writing-review and editing. WW contributed to experimental guidance and methodology. DZ helped in conceptualization, resources, writing-review and editing, and funding acquisition. JZ contributed to conceptualization, writing-review and editing, supervision, project administration, and funding acquisition. RH contributed to methodology and software. MD helped in conceptualization, writing-review and editing, and funding acquisition. ZY contributed to conceptualization and funding acquisition.

Corresponding authors

Correspondence to Jin Zeng or Ming Duan.

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Hu, S., He, R., Wang, W. et al. Composition and co-occurrence patterns of Phragmites australis rhizosphere bacterial community. Aquat Ecol 55, 695–710 (2021). https://doi.org/10.1007/s10452-021-09855-4

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  • DOI: https://doi.org/10.1007/s10452-021-09855-4

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