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Recent Trends in Chiral Separations by 2D-HPLC

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Abstract

2D-HPLC is an important development in chiral separations due to its excellent capability to resolve the enantiomers. This article describes the latest update in the chiral resolution of racemates using 2D-HPLC. The various aspects of the chiral resolution such as validation, optimization, and applications are discussed in both biological and environmental matrices. Some latest developments of nano-2D-HPLC are also included. Besides, efforts are made to discuss the challenges and future perspectives. During the preparation of this article, it was observed that much work has not been done in this direction and only a few papers describe the application of 2D-HPLC in the chiral separation. There is an urgent need to develop more 2D-HPLC methods for a variety of racemates. This article will be useful to the investigators, academicians and industry persons in the future to improve 2D-HPLC for the chiral resolution of the various racemates.

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Fig. 1
Fig. 2
Fig. 3

adapted from Ref. [45]

Fig. 4
Fig. 5

adapted from reference [59] 1(A) and 2(A): achiral separation and 3(A): chiral separation

Fig. 6

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Authors and Affiliations

  1. Department of Chemistry, Kingdom of Saudi Arabia, Taibah University, P.O. Box 30002, Al-Madinah Al-Munawarah, 14177, Kingdom of Saudi Arabia

    Imran Ali

  2. Department of Chemistry, Jamia Millia Islamia Central University, New Delhi, 11025, India

    Imran Ali & Mohd. Suhail

  3. Pharmaceutical and Medicinal Chemistry Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, Dokki, 12311, Cairo, Egypt

    Hassan Y. Aboul-Enein

  4. Department of Inorganic Substances Technology and Electrochemical Processes, Mendeleev University of Chemical Technology, 9 Miusskaya Sq, Moscow, 125047, Russian Federation

    Tatiana Kon’kova

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  1. Imran Ali
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  3. Hassan Y. Aboul-Enein
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Ali, I., Suhail, M., Aboul-Enein, H.Y. et al. Recent Trends in Chiral Separations by 2D-HPLC. Chromatographia 84, 535–548 (2021). https://doi.org/10.1007/s10337-021-04030-1

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