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Organo-metallic pollutants of distillery effluent and their toxicity on freshwater fish and germinating Zea mays seeds

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Abstract

Anaerobically treated molasses-based distilleries spent wash is a major source of terrestrial and aquatic pollution due to the mixture of the various unknown organometallic compounds in discharged waste. The nature of pollutants present in effluent has to be characterized prior to the evaluation of their fate in the environment at various tropic levels. The absorption maxima (λmax) were obtained at 295 nm of pollutants present in spent wash which indicated that melanoidins are a major colorant along with other organic compounds. Abundantly identified compounds with GC–MS in effluent were found: 1,2,4,5-tetrahydro-2-methyl-3H-2-benzazepine-3-one, 6-(trifluoromethyl)-1H-imidazole [4,5-c] pyridine, 4,6-di-t-butyl-1H, 3H-thieno[3,4-c] thiophene, butanoic acid, trimethylsilyl ester, silane, (dodecyloxy)trimethylsilyl ester, 4-styryl [2,2] paracyclophane, 4-(p-cumylphenoxy) phthalonitrile, hexadecanoic acid, trimethylsilyl ester, butyl ester, and squalene. Some of these compounds are known with mutagenic and androgenic properties. The toxicity test of post-methanated distillery effluent (PMDE) showed direct toxic effects on catfish (Heteropneustes fossilis) even at 1% (v/v) concentration and caused degeneration of primary and secondary lamellae of the gill and the epithelial layer. Further, higher concentrations between 5 and 10% of PMDE showed loss of primary and secondary lamellae of gills, and liver catalase activity was also increased dramatically in H. fossilis, indicating the generation of oxidative free radicals. Similarly, inhibition of α-amylase activities in germinating Zea mays L. (Maize) seeds was also noted at 10% PMDE. This study confirmed the environmental toxicity of effluent with freshwater fish and Zea mays seeds.

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Acknowledgements

This research was financially supported by the Department of Biotechnology, Government of India. Vide Letter No. BT/PR13922/BCE/8/1129/2015

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  1. Department of Environmental Microbiology, School of Environmental Sciences, Babasaheb Bhimrao Ambedkar University (A Central University) Vidya Vihar, Raebareli Road, Lucknow, Uttar Pradesh, 226025, India

    S. Tripathi, K. Singh & Ram Chandra

  2. Department of Zoology, School of Life Sciences, Babasaheb Bhimrao Ambedkar University (A Central University) Vidya Vihar, Raebareli Road, Lucknow, Uttar Pradesh, 226025, India

    A. Singh & A. Mishra

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Tripathi, S., Singh, K., Singh, A. et al. Organo-metallic pollutants of distillery effluent and their toxicity on freshwater fish and germinating Zea mays seeds. Int. J. Environ. Sci. Technol. 19, 2025–2038 (2022). https://doi.org/10.1007/s13762-021-03233-1

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