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Comprehensive dynamic analysis of proteins in the spermatheca of female Haemaphysalis longicornis after copulation

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Abstract

Ticks are obligate blood-sucking parasitic arthropods. When sucking the blood of hosts, they can also transmit a variety of pathogens to hosts that severely endanger the health of humans and animals. The spermatheca is an organ for the storage and protection of sperm and an important component of the reproductive system of female ticks. The spermatheca content changes dramatically over time after copulation. In particular, some proteins and polypeptide substances can influence the physiological functions of female ticks and promote blood feeding and egg laying by female ticks. To investigate the molecular mechanisms underlying the productive process of Haemaphysalis longicornis, data-independent acquisition (DIA) quantitative proteomics technology was used to perform in-depth research of the dynamic changes in all proteins in the spermatheca of ticks within a short time after copulation to look for key proteins in the spermatheca contents after copulation that affect the reproduction of female ticks in order to provide meaningful information for the comprehensive prevention and control of ticks.

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Acknowledgements

This work was supported by the Natural Science Foundation for Excellent Youth Scholars of Hebei Province of China (No. C2017205135), the Natural Science Fund for Distinguished Young Scholars of Hebei Normal University (No. L2017J04), and the Open Funds of the State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (No. SKLVEB2018KFKT007).

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Author notes

  1. Jingze Liu

    Present address: Hebei Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology, College of Life Sciences, Hebei Normal University, 20 Nanerhuan East Road, Shijiazhuang, 050024, Hebei, China

  2. Xiaoshuang Wang and Yuhong Hu contributed equally to this work.

Authors and Affiliations

  1. Hebei Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang, 050024, Hebei, China

    Xiaoshuang Wang, Yuhong Hu, Xiaomin Xue, Mengxue Li, Siyang Zhou & Hui Wang

  2. Instrumental Analysis Center, Hebei Normal University, Shijiazhuang, 050024, Hebei, China

    Yuhong Hu & Yanan Han

  3. Department of Pathogenic Biology, College of Basic Medicine, Hebei Medical University, Shijiazhuang, 050017, Hebei, China

    Xiaohong Yang

  4. State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China

    Xiaohong Yang

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  1. Xiaoshuang Wang
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Contributions

HW designed experiments and wrote the initial manuscript. SZ, XW, YH, YH performed experiments. XX, ML prepared figures. JL designed experiments and corrected the manuscript.

Corresponding authors

Correspondence to Hui Wang or Jingze Liu.

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The authors declare that they have no conflict of interest.

Ethical approval

This study was approved by the Animal Ethics Committee of Hebei Normal University (No. 165031) as complying with the animal protection law of the People’s Republic of China.

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Wang, X., Hu, Y., Han, Y. et al. Comprehensive dynamic analysis of proteins in the spermatheca of female Haemaphysalis longicornis after copulation. Exp Appl Acarol 83, 583–596 (2021). https://doi.org/10.1007/s10493-021-00606-6

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  • DOI: https://doi.org/10.1007/s10493-021-00606-6

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