Abstract
Flyingfishes of the family Exocoetidae are sometimes difficult to identify solely based on morphology as members of certain genera share overlapping characteristics. Combining morphological and molecular analyses, the present study reports two specimens of stained flyingfish Cheilopogon spilonotopterus (Bleeker 1865) from the northern Bay of Bengal. As for COI gene sequences, nucleotide variations at five different positions (576 nucleotides identical out of 581 bp) were noted between two specimens of C. spilonotopterus and K2P genetic distances for all conspecific sequences ranged 0.000–0.050. The results of DNA barcoding, therefore, support the validity of both specimens as C. spilonotopterus at genetic level. These are the first DNA barcode records of C. spilonotopterus from the northern Bay of Bengal region.
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Acknowledgements
This study was supported by the Research and Publication Cell, University of Chittagong (ref. 
). The Scientific Research Foundation of Third Institute of Oceanography, MNR (2019017) also contributed to the molecular analysis of this work.
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Fishes were collected as part of faunal survey and no animal testing was performed during this study. Therefore, no ethical approval was required.
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Sharifuzzaman, S.M., Rasid, M.H., Rubby, I.A. et al. DNA barcoding confirms a new record of flyingfish Cheilopogon spilonotopterus (Beloniformes: Exocoetidae) from the northern Bay of Bengal. Conservation Genet Resour 13, 323–328 (2021). https://doi.org/10.1007/s12686-021-01200-z
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DOI: https://doi.org/10.1007/s12686-021-01200-z