Skip to main content
SpringerLink
Log in

Biostimulant priming in Oryza sativa: a novel approach to reprogram the functional biology under nutrient-deficient soil

  • Original Paper
  • Published:
Cereal Research Communications Aims and scope Submit manuscript

Abstract

Biostimulants can be used as an alternative to fertilizers to overcome the effect of nutrient deficiency in plants. Yeast is a biostimulant, having the capacity to modify physiological processes in plants by improving germination and seedling growth under environmental stress. The effects of yeast extract, a sustainable biostimulant derived from yeast (Saccharomyces cerevisiae) containing all the nutritive values, amino acids, vitamins, and growth factors, were investigated in rice (Oryza sativa). Various growth parameters, Chlorophyll a fluorescence, PS II, metabolites accumulation, and antioxidative function of enzymatic (SOD, CAT, APX, GPOX) as well as non-enzymatic (phenol, AsA, and GSH) antioxidant were analyzed in two rice varieties (Hraswa-tolerant and Manu ratna-sensitive) in both yeast extract primed and non-primed seeds subjected to nutrient-deficient soil conditions. The application of the biostimulant (yeast extract) as a priming agent increased the biomass, photosynthetic pigment, modified chlorophyll a fluorescence-related parameters, and also enhanced the photosystem activities of seedlings, supported by decreased content of ROS and MDA in primed seeds when compared to non-primed ones. These changes were accompanied by a significant enhancement in osmolytes and non-enzymatic antioxidants (AsA, GSH, and total phenolics). In general, yeast priming under nutrient-deficient conditions appeared to stimulate the activities of SOD, CAT, and APX in var. Hraswa while GPOX was dramatically upregulated in var. Manu ratna. Thus, these results speculate that yeast priming under nutrient-deficient conditions maintained cell homeostasis and provided better adaptation to the stress conditions in both tolerant as well as sensitive varieties of rice seedlings.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Abdelaal KA, Hafez YM, El Sabagh A, Saneoka H (2017) Ameliorative effects of abscisic acid and yeast on morpho-physiological and yield characteristics of maize plant (Zea mays L.) under water deficit conditions. Fresen Environ Bull 26:7372–7383

    CAS  Google Scholar 

  • Ahmad P, Jaleel CA, Salem MA, Nabi G, Sharma S (2010) Roles of enzymatic and nonenzymatic antioxidants in plants during abiotic stress. Crit Rev Biotechnol 30:161–175

    Article  CAS  Google Scholar 

  • Arnon DI (1949) Copper enzymes in isolated chloroplast. Plant physiol 24:1–15

    Article  CAS  Google Scholar 

  • Bates LS, Waldren RP, Teare LD (1973) Determination of proline for water-stress studies. Plant Soil 39:205–207

    Article  CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of microgram quantities of proteins utilizing the principle of protein dye-binding. Anal Biochem 72:154–248

    Article  Google Scholar 

  • Doke N (1983) Involvement of superoxide anion generation in the hypersensitive response of potato tuber tissues to infection with an incompatible race of Phytophtora infestans and the hyphal wall components. Physiol Plant Pathol 23:345–357

    Article  CAS  Google Scholar 

  • Dubois M, Gilles KA, Hamilton JK, Rebers PT, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356

    Article  CAS  Google Scholar 

  • Gaspar T, Penel C, Greppin H (1975) Peroxidase and isoperoxidase in relation to root and flower formation. J Plant Biochem 2:33–47

    CAS  Google Scholar 

  • Giannopolitis CN, Ries SK (1977) Superoxide dismutases: I. Occurrence in higher plants. Plant Physiol 59(2):309–314

  • Hammad SA, Ali OA (2014) Physiological and biochemical studies on drought tolerance of wheat plants by application of amino acids and yeast extract. Ann Agric Sci 59:133–145

    Article  Google Scholar 

  • Heath RL, Packer L (1968) Phytoperoxidation in isolated chloroplasts. I – kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem 125:189–198

    Article  CAS  Google Scholar 

  • Hussain S, Khan F, Hussain HA, Nie L (2016) Physiological and biochemical mechanisms of seed priming-induced chilling tolerance in rice cultivars. Front Plant Sci 7:116

    PubMed  PubMed Central  Google Scholar 

  • Jarošová M, Klejdus B, Kováčik J, Babula P, Hedbavny J (2016) Humic acid protects barley against salinity. Acta Physiol Plant 38(6)

  • Jeyalakshmi S, Radha R (2017) A review on diagnosis of nutrient deficiency symptoms in plant leaf image using digital image processing. ICTACT J Image Video Process

  • Jisha KC, Vijayakumari K, Puthur JT (2013) Seed priming for abiotic stress tolerance: an overview. Acta Physiol Plant 35:1381–1396

    Article  Google Scholar 

  • Kandlbinder A, Finkemeier I, Wormuth D, Hanitzsch M, Dietz KJ (2004) The antioxidant status of photosynthesizing leaves under nutrient deficiency: redox regulation, gene expression and antioxidant activity in Arabidopsis thaliana. Physiol Plant 120:63–73

    Article  CAS  Google Scholar 

  • Kar M, Mishra D (1976) Catalase, peroxidase, and polyphenoloxidase activities during rice leaf senescence. Plant Physiol 57:315–319

    Article  CAS  Google Scholar 

  • Koleška I, Hasanagić D, Todorović V, Murtić S, Klokić I, Parađiković N, Kukavica B (2017) Biostimulant prevents yield loss and reduces oxidative damage in tomato plants grown on reduced NPK nutrition. J Plant Interact 12(1):209–218

    Article  Google Scholar 

  • Kurtzman CP, Fell JW (2005) Biodiversity and ecophysiology of yeasts. The Yeast Handbook, pp 11–30

  • Lal R (2016) Feeding 11 billion on 0.5 billion hectare of area under cereal crops. Food Energy Secur 5:239–251

    Article  Google Scholar 

  • Law DR, Crafts-Brandner SJ, Salvucci ME (2001) Heat stress induces the synthesis of a new form of ribulose-1, 5-bisphosphate carboxylase/oxygenase activase in cotton leaves. Planta 214:117–125k

    Article  CAS  Google Scholar 

  • Li X, Zhang L (2012) SA and PEG-induced priming for water stress tolerance in rice seedling. In: Information technology and agricultural engineering. Springer, Berlin, Heidelberg, pp 881–887

    Chapter  Google Scholar 

  • Moore S, Stein WH (1948) Photometric ninhydrin method for use in chromatography of amino acids. J Biol Chem 176:367–388

    Article  CAS  Google Scholar 

  • Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880

    CAS  Google Scholar 

  • Oustric J, Morillon R, Luro F, Herbette S, Martin P, Giannettini J, Santini J (2019) Nutrient deficiency tolerance in citrus is dependent on genotype or ploidy level. Front Plant Sci 10:127

    Article  Google Scholar 

  • Ricci M, Tilbury L, Daridon B, Sukalac K (2019) General principles to justify plant biostimulant claims. Front Plant Sci 10:494

    Article  Google Scholar 

  • Sami F, Yusuf M, Faizan M, Faraz A, Hayat S (2016) Role of sugars under abiotic stress. Plant Physiol Biochem 109:54–61

    Article  CAS  Google Scholar 

  • Sarker U, Oba S (2018) Catalase, superoxide dismutase and ascorbate-glutathione cycle enzymes confer drought tolerance of Amaranthus tricolor. Sci Rep 8:1–12

    Google Scholar 

  • Sen A, Puthur JT (2020) Influence of different seed priming techniques on oxidative and antioxidative responses during the germination of Oryza sativa varieties. Physiol Mol Biol Plants 26:551–565

    Article  CAS  Google Scholar 

  • Shalaby MES, El-Nady MF (2008) Application of Saccharomyces cerevisiae as a biocontrol agent against Fusarium infection of sugar beet plants. Acta Biol Szeged 52:271–275

    Google Scholar 

  • Tewari RK, Kumar P, Sharma PN (2007) Oxidative stress and antioxidant responses in young leaves of mulberry plants grown under nitrogen, phosphorus or potassium deficiency. J Integ Plant Biol 49:313–322

    Article  CAS  Google Scholar 

  • Velikova V, Yordanov I, Edreva A (2000) Oxidative stress and some antioxidant systems in acid rain-treated bean plants: protective role of exogenous poly-amines. Plant Sci 151:59–66

    Article  CAS  Google Scholar 

  • Yeo ET, Kwon HB, Han SE, Lee JT, Ryu JC, Byu MO (2000) Genetic engineering of drought resistant potato plants by introduction of the trehalose-6-phosphate synthase (TPS1) gene from Saccharomyces cerevisiae. Mol Cells 10:263–268

    CAS  PubMed  Google Scholar 

  • Yin D, Chen S, Chen F, Guan Z, Fang W (2009) Morphological and physiological responses of two chrysanthemum cultivars differing in their tolerance to waterlogging. Environ Exp Botany 67:87–93

    Article  CAS  Google Scholar 

Download references

Acknowledgement

RJ received financial support from University of Calicut in the form of fellowship.

Author information

Authors and Affiliations

  1. Plant Physiology and Biochemistry Division, Department of Botany, University of Calicut, C.U. Campus P.O., Malappuram, Kerala, 673635, India

    Riya Johnson & Jos T. Puthur

Authors
  1. Riya Johnson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jos T. Puthur
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jos T. Puthur.

Ethics declarations

Conflict of interest

The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Supplementary Information

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Johnson, R., Puthur, J.T. Biostimulant priming in Oryza sativa: a novel approach to reprogram the functional biology under nutrient-deficient soil. CEREAL RESEARCH COMMUNICATIONS 50, 45–52 (2022). https://doi.org/10.1007/s42976-021-00150-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s42976-021-00150-4

Keywords

Search

Navigation