RESEARCH PAPERRapid Visualized Detection of Escherichia Coli O157:H7 by DNA Hydrogel Based on Rolling Circle Amplification
Graphical abstract
Herein, an aptasensor for the rapid visualization detection of E. coli O157: H7 was developed based on magnetic separation, specific identification of aptamers and characteristics of DNA hydrogel prepared by complementary hybridization of two RCA products. The proposed method was simple, rapid and had high sensitivity and specificity for the detection of E. coli O157: H7. In addition, it exhibited great potential to be a universal strategy for monitoring food safety.
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2023, TrAC - Trends in Analytical ChemistryAn electrochemical aptasensor based on dual-enzymes-driven target recycling strategy for patulin detection in apple juice
2022, Food ControlCitation Excerpt :Typically, one or two target molecules only triggers a single biorecognizing event, which limits the sensitivity of the aptasensor. Therefore, a variety of signal amplification strategies have been introduced to aptasensor to improve the utilization rate of the target, including rolling circle amplification (RCA), polymerase chain reaction (PCR), strand displacement amplification (SDA)(Fan et al., 2018; Xia Li et al., 2018; Ma et al., 2019; Zhang, Tao, Bian, Chen, & Yan, 2021). Besides, enzymes-assisted target recycling (EATR) is considered a promising signal amplification mechanism.
Recent advances in microchip-based methods for the detection of pathogenic bacteria
2022, Chinese Chemical LettersCitation Excerpt :Molecular diagnostics play an important role in medical diagnosis, biological studies, food safety, and environmental monitoring. However, the effective detection of low amounts of target nucleic acids in complex biological samples required signal amplification techniques; loop-mediated isothermal amplification (LAMP) [61,62], CHA [39,63], RCA [64,65], and strand displacement amplification (SDA) [66,67], have been developed in this regard. SDA is an isothermal amplification method that generally depends on an ssDNA template containing complementary bases to the endonuclease recognition sequence; however, the SDA method falls short in terms of detecting short nucleic acids.
This work was supported by the National Natural Science Foundation of China (No. 21775102) and the Natural Science Foundation of Shanghai Municipal, China (No. 20ZR1424100).