Abstract
Gynogenetic progeny of sterlet Acipenser ruthenus were obtained by inseminating eggs with UV-inactivated sperm followed by meiotic restoration of ploidy by heat shock. Gynogenetic origin of progeny was assessed by parentage assignment using 15 microsatellite markers. Sex ratio was investigated by gonad histology in 15 3-year-old fish, eight of which were confirmed gynogenotes, with five of those containing primary oocytes (62.5%). Blood plasma concentration of 11-ketotestosterone (KT-11) was analyzed in 63 4-year-old juveniles, 46 of which were genetically confirmed gynogenotes. Measurement of KT-11 showed the proportion of female sterlet to be 65.2%. This sex ratio conformed to the current hypothesis of female heterogametic sex determination but was at the low end of the reported female ratio in sturgeon gynogenetic progeny.
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The data that support the findings of this study are available from the corresponding author upon request.
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This study was financially supported by the Ministry of Education, Biodiversity (CZ.02.1.01/0.0 /0.0/16_025/0007370) and by Czech Science Foundation consequent projects (No. 14-02940S and No. 18-09323S).
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This study was carried out in strict accordance with the Czech Law No. 246/1992 on animal welfare. Protocols have undergone the ethical review process by the University of South Bohemia animal care committee and were approved by the Czech Ministry of Education, Youth and Sports (MSMT-8421/2015-14; in Czech). All surgery was performed under clove oil anesthesia, and all efforts were made to minimize suffering.
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Lebeda, I., Rodina, M., Gela, D. et al. Gonadal histology and concentration of 11-ketotesterone of meiotic gynogens confirm female heterogametic sex determination in sterlet (Acipenser ruthenus). Aquacult Int 29, 801–811 (2021). https://doi.org/10.1007/s10499-021-00658-w
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DOI: https://doi.org/10.1007/s10499-021-00658-w