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Structures of the Mouse Central Nervous System Contain Different Quantities of Proteasome Gene Transcripts

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Abstract

Proteasomes are multisubunit complexes that degrade most intracellular proteins. Three of the 14 subunits of the 20S proteasome, specifically β1, β2, and β5, demonstrate catalytic activity and hydrolyze peptide bonds after acidic, basic, and hydrophobic amino acids, respectively. Within proteasome, the constitutive catalytic subunits β1, β2, and β5 can be substituted by the immune β1i, β2i, and β5i subunits, respectively. However, proteasomes do not always contain all the immune subunits at once; some proteasomes contain both immune and constitutive catalytic subunits simultaneously. Incorporation of immune subunits modifies the pattern of peptides produced by proteasomes. This is essential for antigen presentation and cellular response to stress as well as for a number of intracellular signaling pathways. We have developed a quantitative PCR-based system for the determination of the absolute levels of murine constitutive and immune proteasome subunits gene expression. Using the obtained system, we have estimated the expression levels of genes encoding proteasome subunits in the mouse central nervous system (CNS) tissues. We have shown that the quantity of transcripts of proteasome catalytic subunits in different CNS structures differed significantly. These data allow us to assume that the studied brain regions can be divided into two groups, with relatively “high” (cerebral cortex and spinal cord) and “low” (hippocampus and cerebellum) levels of proteasome subunit genes expression. Moreover, it was possible to distinguish structures with similar and significantly different gene expression profiles of proteasome catalytic subunits. Thus, the gene expression profiles in the cortex, spinal cord, and cerebellum were similar, but different from the expression profile in the hippocampus. Based on the obtained data, we suggest that there are differences in the proteasome pool, as well as in the functional load on the ubiquitin–proteasome system in different parts of the CNS.

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Notes

  1. Abbreviations: CNS, central nervous system; UPS, ubiquitin-proteasome system.

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Funding

This study was supported by the Russian Science Foundation, grant no. 18-74-10095. Animal housing was supported by the Program for Support of Bioresource Collections of the Institute of Physiologically Active Substances, Russian Academy of Sciences, Federal Agency of Scientific Organizations, project no. 0090-2017-0016.

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Authors and Affiliations

  1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991, Moscow, Russia

    S. Yu. Funikov, D. S. Spasskaya, A. V. Burov, V. L. Karpov & A. V. Morozov

  2. Moscow State University, 119991, Moscow, Russia

    A. V. Burov

  3. Institute of Physiologically Active Substances, Russian Academy of Sciences, 142432, Chernogolovka, Moscow oblast, Russia

    E. V. Teterina & A. A. Ustyugov

Authors
  1. S. Yu. Funikov
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  2. D. S. Spasskaya
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  3. A. V. Burov
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  4. E. V. Teterina
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  5. A. A. Ustyugov
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  6. V. L. Karpov
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  7. A. V. Morozov
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Correspondence to A. V. Morozov.

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Conflict of interest. The authors declare no conflict of interest.

Statement on the welfare of animals. The experiments were performed in accordance with the international, national, and/or institutional guidelines for the care and use of animals, and with the Order of the Ministry of Health Care of the Russian Federation no. 199n, April 1, 2016 “On approval of the rules of good laboratory practice.”

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Translated by M. Stepanichev

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Funikov, S.Y., Spasskaya, D.S., Burov, A.V. et al. Structures of the Mouse Central Nervous System Contain Different Quantities of Proteasome Gene Transcripts. Mol Biol 55, 47–55 (2021). https://doi.org/10.1134/S0026893320060047

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