Abstract
Aristeus antennatus is one of the most exploited and economically important resource for fisheries in the Western and Central Mediterranean Sea displaying low population differentiation with mitochondrial and microsatellite markers. The recent development of Genotyping-by-Sequencing (GBS) methods may contribute to the discovery of SNPs and the assessment of genetic differences between populations of this species for fisheries management. Using samples from four geographical sites in Western Mediterranean and Eastern Atlantic, 115,071 putative SNPs were detected. After the stringent quality control measures and the filtering procedure, 232 SNP loci were discovered. Finally, we selected 80 SNP subset panel for Fluidigm Dynamic array application. The results showed significant differentiation among populations from the four sampling sites. Population assignment power and patterns of population differentiation were comparable between the two SNP panels. These markers represent a useful tool for future genetic application of A. antennatus populations.
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Acknowledgements
This work has been funded by Government of Balearic Islands. The authors thank Centro Nacional de Análisis Genómico (CNAG) for technical support. We would like also to thank Margarida Castro for sampling and José Maria Valencia for helping in DNA extraction. G. Catanese is contracted under the INIA/CCAA cooperative research program for postdoctoral incorporation from the Spanish National Institute for Agricultural and Food Research and Technology (INIA).
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Catanese, G., Trotta, J.R., Iriondo, M. et al. Discovery of SNP markers of red shrimp Aristeus antennatus for population structure in Western Mediterranean Sea. Conservation Genet Resour 13, 21–25 (2021). https://doi.org/10.1007/s12686-020-01178-0
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DOI: https://doi.org/10.1007/s12686-020-01178-0