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Salicylic Acid and Iron Nanoparticles Application to Mitigate the Adverse Effects of Salinity Stress Under In Vitro Culture of Strawberry Plants

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Abstract

To explore the impact of salicylic acid (SA) (0, 0.01, and 0.05 mM) and iron nanoparticles (INs) (0, 0.08, and 0.8 ppm) and their interaction on morpho-physiological response of strawberry to salinity stress (0, 50, and 100 mM NaCl), a three-way factorial experiment with three replications was conducted under in vitro condition. Based on the results, salinity not only decreased total dry weight (TWD) and total soluble protein, but also increased the content of hydrogen peroxide (H2O2), malondialdehyde (MDA), proline, and the activity of superoxide dismutase (SOD) and peroxidase (POD) enzymes. Most of the observed changes under control and salinity were explained by principal component analysis (PCA) based on vegetative and physiological traits. The first two components (PC1 and PC2) accounted for 72.8% and 20.4% of the changes, respectively. The highest activity of POD and SOD enzymes as well as the highest content of proline was obtained in the simultaneous presence of 0.05 mM salicylic acid and 0.8 ppm INs. Taken together, the present study revealed that implementation of SA and INs under salinity stress could alleviate the adverse effects of salinity on strawberry under in vitro conditions.

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References

  • Aghaeifard F, Babalar M, Fallahi E, Ahmadi A (2016) Influence of humic acid and salicylic acid on yield, fruit quality, and leaf mineral elements of strawberry (Fragaria× Ananassa Duch.) cv. Camarosa J Plant Nutr 39:1821–1829

    Article  Google Scholar 

  • Ahmad R, Tripathi AK, Tripathi P, Singh S, Singh R, Singh RK (2008) Malondialdehyde and protein carbonyl as biomarkers for oxidative stress and disease progression in patients with chronic myeloid leukemia. In Vivo 22:525–528

    Google Scholar 

  • Askary M, Amirjani MR, Saberi T (2016) Comparison of the effects of nano-iron fertilizer with iron-chelate on growth parameters and some biochemical properties of Catharanthus roseus. J Plant Nutr 40(7):123–143. https://doi.org/10.1080/01904167.2016.1262399

    Article  Google Scholar 

  • Bates L, Waldren R, Teare I (1973) Rapid determination of free proline for water-stress studies. Plant soil 39:205–207

    Article  Google Scholar 

  • Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287

    Article  Google Scholar 

  • Bompadre MJ, Silvani VA, Bidondo LF, Ríos de Molina MDC, Colombo RP, Pardo AG, Godeas AM (2014) Arbuscular mycorrhizal fungi alleviate oxidative stress in pomegranate plants growing under different irrigation conditions. Botany 92:187–193

    Article  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  Google Scholar 

  • Bryla D, Scagel C (2014) Limitations of CaCl2 salinity to shoot and root growth and nutrient uptake in ‘Honeoye’ strawberry (Fragaria× ananassa Duch.). J Hortic Sci Biotechnol 89:458–470

    Article  Google Scholar 

  • Bu R, Xie J, Yu J, Liao W, Xiao X, Lv J, Wang C, Ye J, Calderón-Urrea A (2016) Autotoxicity in cucumber (Cucumis sativus L.) seedlings is alleviated by silicon through an increase in the activity of antioxidant enzymes and by mitigating lipid peroxidation. J Plant Biol 59:247–259

    Article  Google Scholar 

  • Chamani E, Karimi Ghalehtaki S, Mohebodini M, Ghanbari A (2015) The effect of zinc oxide nano particles and humic acid on morphological characters and secondary metabolite production in Lilium ledebourii Bioss. Iran J Genet Plant Breed 4:11–19

    Google Scholar 

  • Chunthaburee S, Sanitchon J, Pattanagul W, Theerakulpisut P (2015) Effects of salt stress after late booting stage on yield and antioxidant capacity in pigmented rice grains and alleviation of the salt-induced yield reduction by exogenous spermidine. Plant Prod Scent 18(1):32–42. https://doi.org/10.1626/pps.18.32

    Article  Google Scholar 

  • Christensen B, Sriskandarajah S, Serek M, Müller R (2008) In vitro culture of Hibiscus rosa-sinensis L.: influence of iron, calcium and BAP on establishment and multiplication. Plant Cell Tissue Organ Cult 93(2):151–161. https://doi.org/10.1007/s11240-008-9354-4

    Article  Google Scholar 

  • Du S-T, Liu Y, Zhang P, Liu H-J, Zhang X-Q, Zhang R-R (2015) Atmospheric application of trace amounts of nitric oxide enhances tolerance to salt stress and improves nutritional quality in spinach (Spinacia oleracea L.). Food Chem 173:905–911

    Article  Google Scholar 

  • El-Kereti MA, El-feky SA, Khater MS, Osman YA, El-Sherbini E-S (2013) Effect of He Ne laser irradiation and Fe3O4 NPs foliar spray on growth and yield of sweet basil plant. Int J Indigenous Medic Plants 46(2):1410–1421

    Google Scholar 

  • Fathi A, Zahedi M, Torabian S, Khoshgoftar A (2016) Response of wheat genotypes to foliar spray of ZnO and Fe2O3 nanoparticles under salt stress. J Plant Nutr 32:223–235

    Google Scholar 

  • Filippou P, Bouchagier P, Skotti E, Fotopoulos V (2014) Proline and reactive oxygen/nitrogen species metabolism is involved in the tolerant response of the invasive plant species Ailanthus altissima to drought and salinity. Environ Exp Bot 97:1–10

    Article  Google Scholar 

  • Flohé L, Günzler WA (1984) [12] Assays of glutathione peroxidase. Methods Enzymol 105:114–120

    Article  Google Scholar 

  • Ghadakchiasl A, Mozafari A.-a, Ghaderi N, (2017) Mitigation by sodium nitroprusside of the effects of salinity on the morpho-physiological and biochemical characteristics of Rubus idaeus under in vitro conditions. Physiol Mol Biol Plants 23:73–83

    Article  Google Scholar 

  • Ghasemi S, Khoshgoftarmanesh AH, Afyuni M, Hadadzadeh H (2014) Iron(II)–amino acid chelates alleviate salt-stress induced oxidative damages on tomato grown in nutrient solution culture. Sci Hortic 165:91–98

    Article  Google Scholar 

  • Gill SS, Anjum NA, Gill R, Tuteja N (2016) Abiotic stress signaling in plants-an overview. Abiot Stress Response Plants 3:424–457. https://doi.org/10.1002/9783527694570.ch1

    Article  Google Scholar 

  • Gupta B, Huang B (2014) Mechanism of salinity tolerance in plants: physiological, biochemical, and molecular characterization. Int J Genomics 2014:234–244

    Article  Google Scholar 

  • Gupta R, Wall T, Baxter L (2007) Impact of mineral impurities in solid fuel combustion. Springer, New York . https://doi.org/10.1007/b118076

    Book  Google Scholar 

  • Harun-Or-Rashid M, Bari M, Islam S (2017) Improvement of cold tolerance efficiency on storage conditions of encapsulated nodal segments of potato using salicylic acid. SKUAST J Res 19:109–114

    Google Scholar 

  • Hossain MA, Bhattacharjee S, Armin S-M, Qian P, Xin W, Li H-Y, Burritt DJ, Fujita M, Tran L-SP (2015) Hydrogen peroxide priming modulates abiotic oxidative stress tolerance: insights from ROS detoxification and scavenging. Front plant sci 6:345–361

    Google Scholar 

  • Jamali B, Eshghi S (2015) Salicylic acid-induced salinity redressal in hydroponically grown strawberry. Commun Soil Sci Plant Anal 46:1482–1493

    Article  Google Scholar 

  • Koc A (2015) Effect of plant growth-promoting bacteria and arbuscular mycorrhizal fungi on lipid peroxidation and total phenolics of strawberry (Fragaria× ananassa’ San Andreas’) under salt stress. Turk J Agric For 39:992–998

    Article  Google Scholar 

  • Koc A, Balci G, Erturk Y, Keles H, Bakoglu N, Ercisli S (2016) Influence of arbuscular mycorrhizae and plant growth promoting rhizobacteria on proline, membrane permeability and growth of strawberry (Fragaria x ananassa) under salt stress. J Appl Bot Food Qual 89:345–366

    Google Scholar 

  • Metsalu T, Vilo J (2015) ClustVis: A web tool for visualizing clustering of multivariate data using principal component analysis and heatmap. Nucleic Acids Res 43(W1):W566–W570. https://doi.org/10.1093/nar/gkv468

    Article  Google Scholar 

  • Mozafari AA, Vafaee Y, Karami E (2015) In vitro propagation and conservation of Satureja avromanica Maroofi-an indigenous threatened medicinal plant of Iran. Physiol Mol Biol Plants 21:433–439

    Article  Google Scholar 

  • Mozafari A, Havas F, Ghaderi N (2017a) Application of iron nanoparticles and salicylic acid in in vitro culture of strawberries (Fragaria× ananassa Duch.) to cope with drought stress. Plant Cell Tissue Org Cult 33:1–13

    Google Scholar 

  • Mozafari A, Havas F, Ghaderi N (2017b) Application of iron nanoparticles and salicylic acid in in vitro culture of strawberries (Fragaria× ananassa Duch.) to cope with drought stress. Plant Cell Tissue Org Cult 132:511–523. https://doi.org/10.1007/s11240-017-1347-8

    Article  Google Scholar 

  • Mozafari A, Dedejani S, Ghaderi N (2018) Positive responses of strawberry (Fragaria × ananassa Duch.) explants to salicylic and iron nanoparticle application under salinity conditions. Plant Cell Tissue Org Cult 134:267–275. https://doi.org/10.1007/s11240-018-1420-y

    Article  Google Scholar 

  • Murashige TC, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  Google Scholar 

  • Niste M, Vidican R, Rotar I, Stoian V, Pop R, Miclea R (2014) Plant nutrition affected by soil salinity and response of rhizobium regarding the nutrients accumulation. J Pro Environ 7:71–75

    Google Scholar 

  • Pati PK, Rath SP, Sharma M, Sood A, Ahuja PS (2006) In vitro propagation of rosea review. Biotechnol Adv 24:94–114

  • Pick E, Mizel D (1981) Rapid microassays for the measurement of superoxide and hydrogen peroxide production by macrophages in culture using an automatic enzyme immunoassay reader. J Immunol Methods 46:211–226

    Article  Google Scholar 

  • Rahman A, Hossain MS, Mahmud J-A, Nahar K, Hasanuzzaman M, Fujita M (2016) Manganese-induced salt stress tolerance in rice seedlings: Regulation of ion homeostasis, antioxidant defense and glyoxalase systems. Physiol Molecul Biol Plants 22:291–306

    Article  Google Scholar 

  • Rajaie M, Tavakoly A-R (2017) Iron and/or acid foliar spray versus soil application of Fe-EDDHA for prevention of iron deficiency in valencia orange grown on a calcareous soil. J Plant Nut 40:150–158

  • Rzepka-Plevnes D, Kulpa D, Gołębiowska D, Porwolik D (2011) Effects of auxins and humic acids on in vitro rooting of strawberry (Fragaria x ananassa Duch.). J Food Agric Environ 9(34):592–595

    Google Scholar 

  • Saed-Moucheshi A, Heidari B, Zarei M, Emam Y, Pessarakli M (2013) Changes in antioxidant enzymes activity and physiological traits of wheat cultivars in response to arbuscular mycorrhizal symbiosis in different water regimes. Iran Agric Res 31:35–50

    Google Scholar 

  • Saed-Moucheshi A, Pakniyat H, Pirasteh-Anosheh H, Azooz M (2014a) Role of ROS as signaling molecules in plants. In: Ahmad P (ed) Reactive oxygen species, antioxidant network and signaling in plants. Springer, New York, pp 585–626

    Google Scholar 

  • Saed-Moucheshi A, Shekoofa A, Pessarakli M (2014b) Reactive oxygen species (ROS) generation and detoxifying in plants. J Plant Nutr 37:1573–1585

    Article  Google Scholar 

  • Sarabi B, Bolandnazar S, Ghaderi N, Tabatabaei SJ (2016) Multivariate analysis as a tool for studying the effects of salinity in different melon landraces at germination stage. Not Bot Hortic Agrobot Cluj Napoca 44:264–271

    Article  Google Scholar 

  • Vafaee Y, Ghaderi N, Khadivi A (2017) Morphological variation and marker-fruit trait associations in a collection of grape (Vitis vinifera L.). Sc Hortic 225:771–782

    Article  Google Scholar 

  • Valentinuzzi F, Mason M, Scampicchio M, Andreotti C, Cesco S, Mimmo T (2015) Enhancement of the bioactive compound content in strawberry fruits grown under iron and phosphorus deficiency. J Sci Food Agric 95:2088–2094

    Article  Google Scholar 

  • Waheed A-M, Madi A (2016) Influence of salicylic acid (SA) and ascorbic acid (ASA) on ‘in vitro’ propagation and salt tolerance of date palm (Phoenix dactylifera L.) cv. ‘Nersy.’ Aust J Crop Sci 10:969

    Article  Google Scholar 

  • Yaghubi K, Ghaderi N, Vafaee Y, Javadi T (2016) Potassium silicate alleviates deleterious effects of salinity on two strawberry cultivars grown under soilless pot culture. Sci Hortic 213:87–95

    Article  Google Scholar 

  • Yaghubi K, Vafaee Y, Ghaderi N, Javadi T (2019) Potassium silicate improves salinity resistant and affects fruit quality in two strawberry cultivars grown under salt stress. Commun Soil Sci Plant Anal 50:1439–1451

    Article  Google Scholar 

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Funding

The research presented was funded by University of Kurdistan (Grant Number: GRC96-00228-2).

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Authors and Affiliations

  1. Department of Horticultural Sciences, Faculty of Agriculture, University of Kurdistan, P. O. Box: 416, Sanandaj, 66177-15175, Iran

    Sajede Dedejani, Ali akbar Mozafari & Nasser Ghaderi

  2. Research Center of Strawberry Breeding and Improvement, Faculty of Agriculture, University of Kurdistan, P. O. Box: 416, Sanandaj, 66177-15175, Iran

    Ali akbar Mozafari & Nasser Ghaderi

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  1. Sajede Dedejani
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  2. Ali akbar Mozafari
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  3. Nasser Ghaderi
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Correspondence to Ali akbar Mozafari.

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Dedejani, S., Mozafari, A.a. & Ghaderi, N. Salicylic Acid and Iron Nanoparticles Application to Mitigate the Adverse Effects of Salinity Stress Under In Vitro Culture of Strawberry Plants. Iran J Sci Technol Trans Sci 45, 821–831 (2021). https://doi.org/10.1007/s40995-021-01082-8

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  • DOI: https://doi.org/10.1007/s40995-021-01082-8

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