Abstract
Objective and design
Diabetic retinopathy (DR) is one of the most serious microvascular complications of diabetes mellitus (DM). MicroRNAs (miRNAs) have been discovered to play a crucial role in DR, but the mechanisms underlying the effects of miR-301a-3p on DR are poorly understood. This paper was designed to explore the possible role of miR-301a-3p in DR.
Methods
The diabetic rat model was established by a single intraperitoneal injection of streptozotocin (STZ). The effects of miR-301a-3p on the biological functions of HRECs were determined through a series of experiments in vitro/vivo.
Results
The results revealed that interference with miR-301a-3p could decrease the expressions of inflammatory factors and apoptosis in the retinal tissue of DR. Furthermore, it can alleviate the oxidative stress in DR serum, reduce VEGF expression, increase endothelial cell marker expression, and inhibit (High Glucose) HG-induced apoptosis of HRECs. Six-transmembrane epithelial antigen of prostate 4 (STEAP4) was the target of miR-301a-3p. All the effects of miR-301a-3p in DR model were reversed by STEAP4 inhibitor.
Conclusion
miR-301a-3p promotes diabetic retinopathy via regulation of STEAP4. The findings in this study may provide a vital reference for the drug research and development in DR treatment.
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Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
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YMW, LJG, and ZLL designed the study and collected the data; XYM, YMW, and LJG participated in analyzing and interpreting the data and were major contributors in writing the manuscript; ZLL critically revised the article. All authors read and approved the final manuscript.
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All animals are received the human care and experimental procedures. All experimental operations performed on all rats were subject to approved by the Ministry of Health of the People’s Republic of China (documentation number 19890503) and Animal Experiment Ethics Committee of Hebei University of Chinese Medicine. Measures were taken to minimize animal suffering.
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Wang, Y., Gao, L., Li, Z. et al. MicroRNA-301a-3p promotes diabetic retinopathy via regulation of six-transmembrane epithelial antigen of prostate 4. Inflamm. Res. 70, 445–457 (2021). https://doi.org/10.1007/s00011-020-01431-0
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DOI: https://doi.org/10.1007/s00011-020-01431-0