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Features of iron accumulation at high concentration in pulcherrimin-producing Metschnikowia yeast biomass

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Abstract

In previous studies it was found that the antimicrobial properties of pulcherrimin-producing Metschnikowia species are related to the formation of a red pigment—pulcherrimin and sequestration of free iron from their growth medium. For strains of Metschnikowia pulcherrima, M. sinensis, M. shaxiensis, and M. fructicola, at a high, ≈80 mg/kg, elemental Fe concentration in agar growth media we observed the essentially different (metal luster, non-glossy rust like, and colored) yeast biomass coatings. For the studied strains the optical and scanning electron microscopies showed the increased formation of chlamydospores that accumulate a red pigment—insoluble pulcherrimin rich in iron. The chlamydospore formation and decay depended on the iron concentration. In this study pulcherrimin in biomass of the selected Metschnikowia strains was detected by Mössbauer spectroscopy. At ≈80 mg/kg elemental Fe concentration the Mössbauer spectra of biomass of the studied strains were almost identical to these of purified pulcherrimin. Iron in pulcherrimin reached ≈1% of biomass by weight which is very high in comparison with elemental Fe percentage in growth medium and is not necessary for yeast growth. The pulcherrimin in biomass was also observed by Mössbauer spectroscopy at lower, ≈5 mg/kg, elemental Fe concentration. Through chemical binding of iron pulcherrimin sequestrates the soluble Fe in the growth media. However, at high Fe concentrations, the chemical and biochemical processes lead to the pulcherrimin accumulation in biomass chlamydospores. When soluble iron is sequestrated or removed from the growth media in this way, it becomes inaccessible for other microorganisms.

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Fig. 1
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taken from places shown by numbers in (a1); c1 M10 and M.p. at 5 mg/kg elemental Fe concentration after 22 h and c2 4 days; d1 M10 at 22 mg/kg elemental Fe concentration after 20 days and Liesegang rings of pulcherrimin around biomass, growth medium thickness is 2–3 mm; d2 same medium, biomass washed from the surface; e M4, M6, M7 (M. pulcherrima) and M10 strains at ≈80 mg/kg elemental Fe concentration after 22 h, and f scheme of biomass coatings accumulating pulcherrimin

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Acknowledgments

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors. The authors are grateful to Z. Kuodis for the FT-IR spectra and to R. Juškėnas for XRD data.

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Authors and Affiliations

  1. Center for Physical Sciences and Technology, Savanorių 231, 02300, Vilnius, Lithuania

    Kęstutis Mažeika & Rasa Garjonytė

  2. Nature Research Center, Akademijos 2, 08412, Vilnius, Lithuania

    Laurynas Šiliauskas, Gražina Skridlaitė, Antanas Matelis, Algimantas Paškevičius & Vytautas Melvydas

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  1. Kęstutis Mažeika
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Mažeika, K., Šiliauskas, L., Skridlaitė, G. et al. Features of iron accumulation at high concentration in pulcherrimin-producing Metschnikowia yeast biomass. J Biol Inorg Chem 26, 299–311 (2021). https://doi.org/10.1007/s00775-021-01853-z

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