Abstract
Background
In recent years, studies have shown that cyfluthrin is harmful to the reproductive system. Lycium barbarum polysaccharide may be a protective agent.
Objective
To investigate the effect of cyfluthrin (Cy) on testosterone B (inhibin B, INHB) in male rats and the intervention of Lycium barbarum polysaccharide (LBP).
Results
Compared with the control group, the mRNA and protein expression levels of INH α and INH βB in the testis tissue decreased, and the difference was statistically significant (P < 0.05). The LBP intervention group can significantly increase the expression level, and the difference is statistically significant (P < 0.05).
Conclusion
The decrease of INHα and INHβB expression caused by Cy exposure indicates that Cy has a toxic effect on the testis of rats, and the addition of LBP has a certain protective effect on the testis of rats.
Similar content being viewed by others
References
Afzalzadeh MR, Ahangarpour A, Amirzargar A, Varnamkhasti MK, Ganjalidarani H (2015) The effect of Vitis vinifera L. juice on serum levels of inhibin B, sperm count in adult male rats. The world Journal of Men’s Health 33:109
Al-Sarar AS, Abobakr Y, Bayoumi AE, Hussein HI, Al-Ghothemi M (2014) Reproductive toxicity and histopathological changes induced by lambda-cyhalothrin in male mice. Environ Toxicol 29:750
Baskar MK, Murthy PB (2018) Acute in vitro neurotoxicity of some pyrethroids using microelectrode arrays. Toxicol In Vitro 47:165
Decanter C et al (2009) Serum inhibin B during controlled ovarian hyperstimulation: an additional criterion for deciding whether to proceed with egg retrieval. FertilSteril 91:2419
Franco AA, Zanardi OZ, Jacob C, de Oliveira M, Yamamoto PT (2017) Susceptibility of Euseius concordis (Mesostigmata: Phytoseiidae) to pesticides used in citrus production systems. ExpApplAcarol 73:61
Gabriele Lenga BEB et al (2018) Pyrethroids used indoor1ambient monitoring of pyrethroids following a pest control operation. RegulToxicolPharmacol 95:385
Goulis DG et al (2008) Serum inhibin-B and follicle stimulating hormone as predictors of the presence of sperm in testicular fine needle aspirate in men with azoospermia. Hormones (Athens, Greece) 7:140
Hocine L et al (2016) The effects of alpha-cypermethrin exposure on biochemical and redox parameters in pregnant rats and their newborns. PesticBiochemPhysiol 134:49
Huang F et al (2016) Cypermethrin induces macrophages death through cell cycle arrest and oxidative stress-mediated JNK/ERK signaling regulated apoptosis. Int J Mol Sci 17:885
Hussien HM, Abdou HM, Yousef MI (2013) Cypermethrin induced damage in genomic DNA and histopathological changes in brain and haematotoxicity in rats: the protective effect of sesame oil. Brain Res Bull 92:76
Jin T, Lin YY, Jin QA, Wen HB, Peng ZQ (2016) Population susceptibility to insecticides and the development of resistance in Bactrocera cucurbitae (Diptera: Tephritidae). J Econ Entomol 109:837
Lei X et al (2020) Lycium barbarum polysaccharides improve testicular spermatogenic function in streptozotocin-induced diabetic rats. Front Endocrinol 11:164
Li YF, Pan C, Hu JX, Li J, Xu LC (2013) Effects of cypermethrin on male reproductive system in adult rats. Biomed Environ Sci 26:201
Liu C et al (2020) Lycium barbarum polysaccharide protects against ethanol-induced spermiotoxicity and testicular degeneration in Immp2l(+/-) mice. ANDROLOGIA 52:e13554
Lu YC, Liang WZ, Kuo CC, Hao LJ, Chou CT, Jan CR (2020) Action of the insecticide cyfluthrin on Ca2+ signal transduction and cytotoxicity in human osteosarcoma cells. Hum Exp Toxicol 39:1268
Luo Q et al (2014) The effect of Lycium barbarum polysaccharides on the male rats׳ reproductive system and spermatogenic cell apoptosis exposed to low-dose ionizing irradiation. J Ethnopharmacol 154:249
Manzoor SM et al (2012) Serum inhibin B as a diagnostic marker of male infertility. J Ayub Med Coll Abbottabad 24:113
Maurya SK, Mishra J, Abbas S, Bandyopadhyay S (2016) Cypermethrin Stimulates GSK3beta-dependent abeta and p-tau proteins and cognitive loss in young rats: reduced HB-EGF signaling and downstream neuroinflammation as critical regulators. MolNeurobiol 53:968
Mehrpour O, Karrari P, Zamani N, Tsatsakis AM, Abdollahi M (2014) Occupational exposure to pesticides and consequences on male semen and fertility: a review. ToxicolLett 230:146
Mohaf MMS, Abdel-Hami S, Mossa HFHA (2016) Adverse effects of sixty days sub-chronic exposure to β-cyfluthrinon male rats. J Environ SciTechnol 10:1
Mylonas I et al (2009) Development and characterisation of an antibody for the immunohistochemical detection of inhibin/activinbetaE (βE) in normal human ovarian and placental tissue. ActaHistochem 111:367
Qian L, Yu S (2016) Protective effect of polysaccharides fromLycium barbarum on spermatogenesis of mice with impaired reproduction system induced by cyclophosphamide. Am J ReprodImmunol 76:383
Qu Y et al (2017) Sublethal and hormesis effects of beta-cypermethrin on the biology, life table parameters and reproductive potential of soybean aphid Aphis glycines. Ecotoxicology 26:1002
Rodriguez JL et al (2018) Bioavailability and nervous tissue distribution of pyrethroid insecticide cyfluthrin in rats. Food ChemToxicol 118:220
Scott JG, Yoshimizu MH, Kasai S (2015) Pyrethroid resistance in Culex pipiens mosquitoes. PesticBiochemPhysiol 120:68
Seifertova M et al (2017) Determination of selected neurotoxic insecticides in small amounts of animal tissue utilizing a newly constructed mini-extractor. Anal BioanalChem 409:6015
Sharma P, Huq A, Singh R (2014) Cypermethrin-induced reproductive toxicity in the rat is prevented by resveratrol. J Human ReprodSci 7:99
Syed F, Chandravanshi LP, Khanna VK, Soni I (2016) Beta-cyfluthrin induced neurobehavioral impairments in adult rats. Chem-Biol Interact 243:19
Taib IS et al (2013) Fenitrothion induced oxidative stress and morphological alterations of sperm and testes in male Sprague-Dawley rats. Clinics 68:93
Tanemura A, Nagasawa T, Inui S, Itami S (2005) LRIG-1 provides a novel prognostic predictor in squamous cell carcinoma of the skin: immunohistochemical analysis for 38 cases. DERMATOL SURG 31:423
Tang Z et al (2017) Lycium barbarum polysaccharide alleviates nonylphenol exposure induced testicular injury in juvenile zebrafish. Int J BiolMacromol 104:618
Walton KL, Makanji Y, Robertson DM, Harrison CA (2011) The synthesis and secretion of inhibins. VITAM HORM 85:149
Wang XZ et al (2009) Beta-cypermethrin impairs reproductive function in male mice by inducing oxidative stress. Theriogenology 72:599
Wang Q et al (2015) Antagonism effects of cypermethrin on interleukin-6-induced androgen receptor activation. Environ ToxicolPharmacol 40:172
Wang X et al (2017) beta-Cypermethrin and its metabolite 3-phenoxybenzoic acid exhibit immunotoxicity in murine macrophages. ActaBiochimBiophys Sin (Shanghai) 49:1083
Xin Y et al (2012) Protective effect of Lycium barbarum polysaccharides against doxorubicin-induced testicular toxicity in rats. Phytother Res 26:716
Yan Y et al (2018) Efficient preparative separation of beta-cypermethrin stereoisomers by supercritical fluid chromatography with a two-step combined strategy. J SEP SCI 41:1442
Yun X et al (2017) A comparative assessment of cytotoxicity of commonly used agricultural insecticides to human and insect cells. Ecotoxicol Environ Saf 137:179
Zhang J, Zhu W, Zheng Y, Yang J, Zhu X (2008) The antiandrogenic activity of pyrethroid pesticides cyfluthrin and beta-cyfluthrin. Reprod Toxicol 25:491
Zhou YJ et al (2018) Exposure to beta-cypermethrin impairs the reproductive function of female mice. RegulToxicolPharmacol 95:385
Acknowledgements
This work was supported by the National Natural Science Foundation of China (81960584).
Author information
Authors and Affiliations
Contributions
ZG designed and executed the research and wrote the manuscript. YX contributed to animal experiments, and CG and JW participated in the statistical analysis of the data. HY revised the manuscript and coordinated research. All authors have read and approved the final version of the manuscript.
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no competing interests.
Human and animal rights
The animal use protocol listed below has been reviewed and approved by the Laboratory Animal Ethical and Welfare Committee of laboratory Animal Center, Ningxia Medical University.(Approval No. IACUC-NYLAC-2019–076).
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Guo, X., Xie, YX., Guo, C. et al. The effect of cyfluthrin on testis inhibin B in rats and the intervention of Lycium barbarum polysaccharide. Mol. Cell. Toxicol. 17, 151–160 (2021). https://doi.org/10.1007/s13273-021-00120-1
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13273-021-00120-1