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Meiobenthic nematode Oncholaimus campylocercoides as a model in laboratory studies: selection, culture, and fluorescence microscopy after exposure to phenanthrene and chrysene

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Abstract

Numerous studies have focused on the response of meiofauna after exposure to polycyclic aromatic hydrocarbons (PAHs), but none has been devoted to their uptake into nematode body compartments. The present study monitored PAH uptake by Oncholaimus campylocercoides which was selected after 40 days in the laboratory through original protocols from natural sediments collected in the Old Harbor of Bizerte, Tunisia. To achieve the mono-species level, a grain size magnification was applied by gradually adding a biosubstrate made from either the crushed shells of Mytilus galloprovincialis or minced leaves of Posidonia oceanica. After selection, O. campylocercoides was cultured and fed with earthworm powder (560 mg.l-1). Thereafter, it was exposed for 3 weeks to phenanthrene and chrysene (38, 116, and 348 ppb). Fluorescence microscopy revealed higher intensities of PAHs at the spicules, mouths, and pharynges compared with the other organs considered. Moreover, the buccal fluorescence showed a significant correlation with that measured in the biosubstrate made with shells of M. galloprovincialis.

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Acknowledgments

The authors extendtheir appreciation to Researchers Supporting Project number RSP-2021/17, King Saud University, Riyadh, Saudi Arabia. The authors also thank Dr. Mateusz Płóciennik (University of Łódz, Poland) for the valuable assistance during the fluorescence examinations.

Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Funding

The authors extendtheir appreciation to Researchers Supporting Project number RSP-2021/17, King Saud University, Riyadh, Saudi Arabia.

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Authors and Affiliations

  1. Faculty of Sciences of Bizerte, Laboratory of Environment Biomonitoring, Coastal Ecology and Ecotoxicology Unit, University of Carthage, 7021, Zarzouna, Tunisia

    Mohamed Allouche, Ahmed Nasri, Hamouda Beyrem & Fehmi Boufahja

  2. Zoology Department, College of Science, King Saud University, Box 2455, Riyadh, 11451, Saudi Arabia

    Abdel Halim Harrath, Lamjed Mansour & Saleh Alwasel

  3. Faculty of Biology, Alexandru Ioan Cuza University of Iasi, Iasi, Romania

    Gabriel Plăvan

  4. Texas Water Development Board, 1700 North Congress Avenue, Austin, TX, 78701, USA

    Melissa Rohal-Lupher

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  1. Mohamed Allouche
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Contributions

Mohamed Allouche: formal analysis, validation, writing (original draft), writing (review and editing). Ahmed Nasri: data curation, investigation. Abdel Halim Harrath: funding acquisition, writing (review and editing). Lamjed Mansour: funding acquisition, writing (review and editing). Saleh Alwasel: funding acquisition, investigation. Hamouda Beyrem: data curation, investigation. Gabriel Plăvan: investigation. Melissa Rohal-Lupher: writing (review and editing). Fehmi Boufahja: conceptualization, formal analysis, funding acquisition, investigation, methodology, supervision, validation, writing (review and editing).

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Correspondence to Fehmi Boufahja.

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Allouche, M., Nasri, A., Harrath, A.H. et al. Meiobenthic nematode Oncholaimus campylocercoides as a model in laboratory studies: selection, culture, and fluorescence microscopy after exposure to phenanthrene and chrysene. Environ Sci Pollut Res 28, 29484–29497 (2021). https://doi.org/10.1007/s11356-021-12688-1

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