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Molecular cloning and characterization of three CoIDA genes in Camellia oleifera

  • Genetics & Evolutionary Biology - Original Article
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Abstract

The INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) controls floral organ abscission in plants. IDA belongs to IDA-LIKE (IDL) gene family that is involved in regulation of Arabidopsis development. Herein, we identified three genes, CoIDA1, CoIDA2 and CoIDA3 in Camellia oleifera (Camellia oleifera Abel. cv. Huashuo) and suggested their involvement in the regulation of fruits abscission. The full-length cDNA sequences of CoIDA1, CoIDA2 and CoIDA3 were of 207 bp, 276 bp and 273 bp, encoding proteins of 68, 91 and 90 amino acids, respectively. These CoIDA genes were single exon genes (SEGs) with a conserved extended PIP motif (EPIP) at C-terminal that has been implicated to play an important role in governing protein function for enhanced flower abortion rate. The highest expression of CoIDA1 was in young peduncles and the lowest in young fruits. However, the highest expressions of CoIDA2 and CoIDA3 were both in young roots, and the lowest in young fruits. The expressions of CoIDA1 and CoIDA2 significantly increased in abscission zones (AZs) of both abnormal fruits (AF) and ethephon treated fruits (ETH-F) with respect to normal fruits (NF), which suggest that CoIDA1 and CoIDA2 genes are related to fruits abscission in C. oleifera. This study provided a preliminary understanding about CoIDA genes which could lead to their detailed functional analysis and utilization for improving C. oleifera yield potential.

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Acknowledgements

This work was supported by The National Key R&D Program of China (2018YFD1000603), the Scientific Research Starting Foundation for introduce of talents of Central South University of Forestry and Technology (2019YJ003) and the Natural Science Foundation of Hunan Province, China (2018JJ3378).

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Author notes

  1. Mi Yang and Xiao Hu have contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees of Ministry of Education, Central South University of Forestry and Technology, Changsha, 410004, China

    Yang Mi, Hu Xiao, Ma Xiaoling & Yuan Deyi

  2. Key Laboratory of Non-Wood Forest Products of Forestry Ministry, Central South University of Forestry and Technology, Changsha, 410004, China

    Yang Mi, Hu Xiao, Ma Xiaoling & Yuan Deyi

  3. State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha, 410125, People’s Republic of China

    Ouyang Xiang

  4. Department of Biosciences, COMSATS University Islamabad (CUI), Park Road, Islamabad, 45550, Pakistan

    Muhammad Sajjad

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  1. Yang Mi
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  2. Hu Xiao
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  3. Ouyang Xiang
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  4. Muhammad Sajjad
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  5. Ma Xiaoling
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  6. Yuan Deyi
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Contributions

DYY and XLM conceived and designed the experiments. Material preparation, data collection and analysis were performed by MY and XH. The first draft of the manuscript was written by MY and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Ma Xiaoling or Yuan Deyi.

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Yang, M., Hu, X., Ouyang, X. et al. Molecular cloning and characterization of three CoIDA genes in Camellia oleifera. Braz. J. Bot 44, 391–400 (2021). https://doi.org/10.1007/s40415-020-00691-8

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  • DOI: https://doi.org/10.1007/s40415-020-00691-8

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