Original articleDesigning and constructing a phage display synthesized single domain antibodies library based on camel VHHs frame for screening and identifying humanized TNF-α-specific nanobody
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Graphical abstract
The solubility and Immunogenicity of protein significantly impacted the production efficiency and medical application of Nbs. The soluble and humanized framework was rationally designed through bioinformatics analysis. The phagemids carrying genetic diversity of Nb were electro-transformed into TG1 cells for library construction and isolated the binding TNF-α function of Nb. Protein products of TNF-α specific Nb were prepared with E.coli expression system and its bioactivity was verified by cytotoxicity assay.
Keywords
Single domain antibodies
Phage display library
Designing and constructing
Humanized TNF-α-specific Nb
Screening and identifyin
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These authors contributed equally to this work.
© 2021 Published by Elsevier Masson SAS.