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Thiamine-producing lactic acid bacteria and their potential use in the prevention of neurodegenerative diseases

  • Applied microbial and cell physiology
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Abstract

Thiamine or vitamin B1, an essential micronutrient mainly involved in energy production, has a beneficial impact on the nervous system, and its deficiency can be associated with the development and progression of neurodegenerative diseases. The aim of this work was to select thiamine-producing lactic acid bacteria (LAB) and study their physiological effects using neuron cell cultures. In this study, 23 LAB able to produce thiamine were identified by growth in thiamine-free synthetic medium. Intra- and extracellular thiamine concentrations were determined using a microbiological method and results confirmed by HPLC techniques. A wide variation in vitamin production was found showing that this property was not only species specific but also a strain-dependent trait. Five of these strains were pre-selected for their capacity to produce higher concentrations of thiamine. Only the pre-treatment with the intracellular extract of Lactiplantibacillus (L.) plantarum CRL 1905 increased significantly neuronal survival in N2a cells’ model of neurotoxicity (MPP+) with thiamine deficiency conditions (amprolium). Furthermore, amprolium-resistant variants of CRL 1905 were isolated by exposition of the strain to increasing concentrations of this toxic thiamine analogue. The variant A9 was able to increase more than 2 times the intracellular thiamine production of the original strain. A9 bacterial extract significantly prevented neuronal cell death and the increase of IL-6. The amprolium-resistant strain A9 showed a modulating and neuroprotective effect in an in vitro model of neurotoxicity constituting a potential bio-strategy to counteract thiamine deficiencies and thus prevent or treat neurodegenerative diseases.

Key Points

• LAB can produce thiamine in a species- and strain-dependant manner.

• L. plantarum CRL 1905 significantly reduce MPP + -induced neurotoxicity in N2a cells.

• Amprolium-resistant strain A9 has neuroprotective effect and prevents IL-6 increase.

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Funding

This study was funded by the CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas, project PIP 2015-0697), the ANPCyT (Agencia Nacional de Promoción Científica y Tecnológica, project PICT 2016-03010), and CYTED (Programa Iberoamericano de Ciencia y Tecnologia para el Desarrollo, Project 917PTE0537).

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All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by MMT and AdeMdeL. The first draft of the manuscript was written by MMT, and all authors made corresponding revisions. All authors read and approved the final manuscript.

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Correspondence to Jean Guy LeBlanc.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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The authors declare no competing interests.

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Teran, M.d.M., de Moreno de LeBlanc, A., Savoy de Giori, G. et al. Thiamine-producing lactic acid bacteria and their potential use in the prevention of neurodegenerative diseases. Appl Microbiol Biotechnol 105, 2097–2107 (2021). https://doi.org/10.1007/s00253-021-11148-7

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  • DOI: https://doi.org/10.1007/s00253-021-11148-7

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