Abstract
The cereal cyst nematodes (CCN) severely threaten wheat production in many cereal-growing areas around the world as well as in Turkey. The screening of wheat and/or barley varieties for characterization of resistance against CCN is a critical step to develop resistant varieties. The present study describes the phenotyping and genotyping resistance situation of indigenous wheat varieties against one Kilis population of Heterodera latipons. Some barley varieties were also included in the phenotyping test. Among the phenotypically tested wheat varieties, Adana99, Menemen, Troya, Izmir, and Kakliç88 were highly resistant (females/plant < 3) to Kilis population whereas no barley variety was resistant. The microsatellite markers linked to Cre1, Cre3, and Cre5 were detected in the resistant varieties Adana99, Izmir, Ceyhan99, and Ata81, while the presence of Cre8 and CreX genes could not be detected in the varieties tested. As expected, no Cre marker was detected in any barley varieties. The presence of the resistance genes Cre1 and Cre5 in wheat does not adequately suppress the reproduction of H. latipons in Ceyhan99 and Ata81 varieties. The Cre3 gene detected using Xgwm301 marker provides resistance to H. latipons in İzmir and Ziyabey98 wheat varieties. Some Cre genes were detected in some wheat varieties regardless of whether they are phenotypically resistant or not; however, no correlation was found between the presence of any marker and phenotypic resistance.
Similar content being viewed by others
References
Akar T, Caliskan M, Nicol JM, Uranbey S, Sahin E et al (2009) Molecular characterization of cereal cyst nematode diagnostic markers Cre1 and Cre3 in some winter wheat germplasm and their potential use against Heterodera filipjevi. Field Crop Res 114:320–323. https://doi.org/10.1016/j.fcr.2009.08.001
Ali MA, Shahzadi M, Zahoor A, Dababat AA, Toktay H et al (2019) Resistance to cereal cyst nematodes in wheat and barley: an emphasis on classical and modern approaches. Int J Mol Sci 20(2):432. https://doi.org/10.3390/ijms20020432
Caliskan M, Uranbey S, Akar NJ, T, Elekçioğlu H, et al (2011) Indirect selection of Cre1 gene in winter wheat populations. Arch Biol Sci 63:49–53. https://doi.org/10.2298/ABS1101049C
Cui L, Gao X, Wang XM, Jian H, Tang WH et al (2012) Characterization of interaction between wheat roots with different resistance and Heterodera filipjevi. Acta Agron Sin 38:1009–1017. https://doi.org/10.3724/SP.J.1006.2012.01009
Cui JK, Huangpengh WK, Liuwanggao SM, Wang GF, Kong LA et al (2015) A new pathotype characterization of Daxing and Huangyuan populations of cereal cyst nematode (Heterodera avenae). J Integr Agric 14:724–731. https://doi.org/10.1016/S2095-3119(14)60982-5
Dababat AA, Fourie H (2018) Nematode parasites of cereals. In: Sikora RA, Coyne D, Hallmann J, Timper P (eds) Plant Parasitic Nematodes in Subtropical and Tropical Agriculture. CAB International, New York NY USA, pp 163–221
Dababat AA, Erginbas-Orakçı G, Toktay H, İmren M, Akın B (2014) Resistance of winter wheat to Heterodera filipjevi in Turkey. Turk J Agric For 38:180–186. https://doi.org/10.3906/tar-1305-47
Dababat AA, Imren M, Erginbas-Orakci G, Ashrafi S, Yavuzaslanoglu E, Toktay H et al (2015) The importance and management strategies of cereal cyst nematodes, Heterodera spp., in Turkey. Euphytica 202:173–188. https://doi.org/10.1007/s10681-014-1269-z
Dababat AA, Ferney GBH, Erginbas-Orakci G, Dreisigacker S, Imren M et al (2016) Association analysis of resistance to cereal cyst nematodes (Heterodera avenae) and root lesion nematodes (Pratylenchus neglectus and P. thornei) in CIMMYT advanced spring wheat lines for semi-arid conditions. Breed Sci 66:692–702. https://doi.org/10.1270/jsbbs.15158
Dababat AA, İmren M, Özer G, Mokrini F, Duman N et al (2020) Genetic and pathogenic variation in Heterodera latipons populations from Turkey. Nematology 23(1):47–56. https://doi.org/10.1163/15685411-bja10029
de Majnik J, Ogbonnaya FC, Moullet O, Lagudah ES (2003) The Cre1 and Cre3 nematode resistance genes are located at homeologous loci in the wheat genome. Mol Plant Microbe Interact 16:1129–1134. https://doi.org/10.1094/MPMI.2003.16.12.1129
Delibes A, Romero D, Aguaded S, Duce A, Mena M et al (1993) Resistance to the cereal cyst nematode (Heterodera avenae Woll.) transferred from the wild grass Aegilops ventricosa to hexaploid wheat by a “stepping-stone” procedure. Theor Appl Genet 87:402–408. https://doi.org/10.1007/BF01184930
Imren M, Waeyenberge L, Viaene N, Toktay H, Dababat A et al (2012) Molecular characterization of cereal cyst nematodes from the South Anatolian Region in Turkey using ITS-rDNA sequences. J Turk Ent 36:491–499
Imren M, Waeyenberge L, Viaene N, Elekçioğlu İH, Dababat A (2015) Morphological and molecular identification of cereal cyst nematodes from the Eastern Mediterranean region of Turkey. Turk J Agric For 39:91–98. https://doi.org/10.3906/tar-1404-56
Imren M, Waeyenberge L, Koca AS, Duman N, Yildiz Ş et al (2017) Genetic variation and population dynamics of the cereal cyst nematode, Heterodera filipjevi in wheat areas of Bolu, Turkey. Trop Plant Pathol 42:362–369
Imren M, Yildiz Ş, Çiftçi V, Dababat AA (2020) Effect of cereal cyst nematode Heterodera filipjevi on wheat yields in Turkey. Turk J Agric For 44:39–45. https://doi.org/10.3906/tar-1902-17
Jahier J, Abelard P, Tanguy M, Dedryver F, Rivoal R et al (2001) The Aegilops ventricosa segment on chromosome 2AS of the wheat cultivar ‘VPM1’carries the cereal cyst nematode resistance gene Cre5. Plant Breed 120:125–128. https://doi.org/10.1046/j.1439-0523.2001.00585.x
Li XH, Ma J, Gao B, Wang RY, Chen SL (2013) Resistance of wheat cultivars or germplasm lines to Heterodera avenae. J Triticeae Crops 33:1277–1283
Marshall JM, Smiley RW (2016) Spring barley resistance and tolerance to the cereal cyst nematode Heterodera avenae. Plant Dis 100:396–407. https://doi.org/10.1094/PDIS-05-15-0498-RE
Mokabli A, Valette S, Gauthier JP, Rivoal R (2002) Variation in virulence of cereal cyst nematode populations from North Africa and Asia. Nematology 4:521–525. https://doi.org/10.1163/156854102760290491
Nicol JM, Rivoal R (2008) Global Knowledge And Its Application For The Integrated Control And Management Of Nematodes On Wheat. In: Ciancio A, Mukerji KG (eds) Integrated Management and Biocontrol of Vegetable and Grain Crops Nematodes Integrated Management of Plant Pests and Diseases, vol 2. Springer, Dordrecht, pp 251–294. https://doi.org/10.1007/978-1-4020-6063-2_13
Nicol JM, Bolat N, Sahin E, Tülek A, Yıldırım AF et al (2006) The cereal cyst nematode is causing economic damage on rain-fed wheat production systems of Turkey. Phytopathology 96(6):169
Nicol JM, Turner SJ, Coyne DL, den Nijs L, Hockland S et al (2011) Current nematode threats to world agriculture. In: Jones J, Gheysen G, Fenoll C (eds) Genomics and molecular genetics of plant-nematode interactions. Springer, Dordrecht, pp 21–43. https://doi.org/10.1007/978-94-007-0434-3_2
Ogbonnaya FC, Seah S, Delibes A, Jahier J, López-Braña I et al (2001) Molecular-genetic characterization of a new nematode resistance gene in wheat. Theor Appl Genet 102:623–629. https://doi.org/10.1007/s001220051689
Peng J, Richards DE, Hartley NM, Murphy GP, Devos KM et al (1999) Green revolution’genes encode mutant gibberellin response modulators. Nature 400:256–261. https://doi.org/10.1038/22307
Philis I (1988) Occurrence of Heterodera latipons on barley in Cyprus. Nematol Mediterr 16:223
Rivoal R, Cook R (1993) Nematode pests of cereals. In: Evans K, Trudgill DL, Webster JM (eds) Plant parasitic nematodes in temperate agriculture. CAB International, Oxford, pp 259–303
Safari E, Gororo NN, Eastwood RF, Lewis J, Eagles HA et al (2005) Impact of Cre1, Cre8 and Cre3 genes on cereal cyst nematode resistance in wheat. Theor Appl Genet 110:567–572. https://doi.org/10.1007/s00122-004-1873-8
Sahin E, Nicol JM, Yorgancilar A, Elekcioglu IH, Tulek A et al (2008) Seasonal variation of field populations of Heterodera filipjevi, Pratylenchus thornei and P. neglectus on winter wheat in Turkey. Nematol Mediterr 36:51–56
Scholz U, Sikora R (2004) Hatching behaviour and life cycle of Heterodera latipons Franklin under Syrian agro-ecological conditions. Nematology 6(2):245–256. https://doi.org/10.1163/1568541041217924
Seah S, Miller C, Sivasithamparam K, Lagudah ES (2000) Root responses to cereal cyst nematode (Heterodera avenae) in hosts with different resistance genes. New Phytol 146:527–533. https://doi.org/10.1046/j.1469-8137.2000.00654.x
Sewell R (1973) Plant-parasitic nematodes from Canada and abroad, 1971. Can Plant Dis Surv 53:34–35
Sikora RA (1988) Plant parasitic nematodes of wheat and barley in temperate and temperate semiarid regions – a comparative analysis. In: Sikora RA, Srivastava JP (eds) Saxena MG. Nematodes Parasitic to Cereals and Legumes in Temperate Semi-Arid Regions. Aleppo. Syria, ICARDA, pp 46–48
Smiley RW (2014) Resistance of wheat to a Pacific Northwest population of Heterodera avenae, 2013. Plant Dis Manag Rep 8:CF039
Smiley RW, Gui PY, John NP (2011) Resistance of wheat, barley and oat to Heterodera avenae in the Pacific Northwest, USA. Nematology 13:539–552. https://doi.org/10.1163/138855410X531862
Smiley RW, Dababat AA, Iqbal S, Jones MGK, Maafi ZT et al (2017) Cereal cyst nematodes: a complex and destructive group of Heterodera species. Plant Dis 101:1692–1720. https://doi.org/10.1094/PDIS-03-17-0355-FE
Subbotin SA, Rumpenhorst HJ, Sturhan D (1996) Morphological and electrophoretic studies on populations of the Heterodera avenae complex from the former USSR. Russ J Nematol 4:29–38
Subbotin SA, Mundo-Ocampo M, Baldwin JG (2010) Systematics of Cyst Nematodes (Nematoda: Heteroderinae), Part A. In: Hunt, DJ Perry, R (eds) Nematology Monographs and Perspectives. Brill Leiden, Boston, 352 pp. https://doi.org/10.1163/ej.9789004162259.i-352
Talatschian P, Akhiani A, Grayeli Z, Shahmohammadi M, Teimuri F (1976) Survey on cyst forming nematodes in Iran in 1975 and their importance. Iran J Plant Pathol 12:42–43
Tanksley SD, Young ND, Paterson AH, Bonierbale MW (1989) RFLP mapping in plant breeding: new tools for an old science. Bio/Technology 7:257–264. https://doi.org/10.1038/nbt0389-257
Toktay T, Imren M, Ocal A, Waeyenberge L, Viaene N (2015) Incidence of cereal cyst nematodes in the East Anatolia Region in Turkey. Russ J Nematol 23:29–40
Vanstone VA, Hollaway GJ, Stirling GR (2008) Managing nematode pests in the southern and western regions of the Australian cereal industry: continuing progress in a challenging environment. Australas Plant Pathol 37:220–234. https://doi.org/10.1071/AP08020
Zhang RQ, Feng YX, Li HF, Yuan H, Junli D et al (2016) Cereal cyst nematode resistance gene CreV effective against Heterodera filipjevi transferred from chromosome 6VL of Dasypyrum villosum to bread wheat. Mol Breed 36:122. https://doi.org/10.1007/s11032-016-0549-9
Acknowledgements
We gratefully acknowledge the contribution of the Scientific and Technological Council of Turkey (TUBITAK) under 115 R 006 coded project.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Ethical approval
This article does not contain any studies with human participants or animals performed by any of the authors.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Imren, M., Ozer, G., Duman, N. et al. Phenotypic and genotypic characterization of wheat and barley varieties for resistance to cereal cyst nematode (Heterodera latipons). Genet Resour Crop Evol 68, 2131–2141 (2021). https://doi.org/10.1007/s10722-021-01125-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10722-021-01125-4