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Morphological, cytological and phytochemical studies in naturally occurring diploid and tetraploid populations of Physochlaina praealta from high altitudes of Trans-Himalaya

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Abstract

Physochlaina praealta samples were studied macromorphologically and cytomorphologically along with their detailed phytochemical investigation. The concentration of phytoconstituents showed a strong positive correlation with the ploidy level and altitudinal gradients. The total phenol content was detected maximum in the methanolic extract of leaves and stem of higher altitudinal plants in both cytotypes (2x, 4x). The maximum content of flavonoids was detected in the methanolic extract of root and leaves. Root organ from higher elevation possessed the highest DPPH radical scavenging activity, with the maximum percentage of inhibition being obtained in methanolic extracts. The plants of both cytotypes from higher elevations accumulate an abundant quantity of secondary metabolites. The two cytotypes differ from each other with respect to various morphometric characters thereby depicting the drastic affect of polyploidy.

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Abbreviations

FCR:

Folin–Ciocalteu reagent

AlCl3 :

Aluminum chloride

DPPH:

1,1-diphenyl-2-picrylhydrazyl

AT:

Atropine

CA:

Caffeic acid

CHA:

Chlorogenic acid

KOH:

Potassium hydroxide

QE:

Quercetin

TPC:

Total phenol content

TFC:

Total flavonoid content

HPTLC:

High-performance thin-layer chromatography

LOD:

Limit of detection

LOQ:

Limit of quantification

SD:

Standard deviation

PK:

Panikher

MB:

Mulbekh

SP:

Sapi

KH:

Khardung

HU:

Hundur

ICH:

The International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use

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Acknowledgements

Authors wish to thank the University Grant Commission, New Delhi for providing financial assistance under the DRS, SAP I, II & III, and ASSIST Programme and also awarding Junior Research Fellowship to Younas Rasheed Tantray (Award Letter No. 2121430298 12/8/2015). Authors are also thankful to Head, Department of Botany, Punjabi University, Patiala for providing necessary laboratory, herbarium, and library facilities. Thanks are also due to In-charge IPLS-DBT project (BT/PR 4548/INF/22/146/2012) for laboratory facilities.

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Authors and Affiliations

  1. Punjabi University, Patiala, 147002, India

    Younas Rasheed Tantray, Saroj Kumar Pradhan, Vijay Kumar Singhal & Raghbir Chand Gupta

  2. Govt. Degree College, Surankote, Jammu, 185121, India

    Mohammad Saleem Wani

  3. Centre for Biodiversity and Taxonomy, Department of Botany, University of Kashmir, Srinagar, 190006, India

    Maroof Hamid & Ishrat Jan

  4. Department of Zoology, University of Kashmir, Srinagar, 190006, India

    Ishrat Jan

  5. Biology Department, Faculty of Science at Yanbu, Taibah University, King Khalid Rd., Al Amoedi, Yanbu El-Bahr, 46423, Saudi Arabia

    Talaat H. Habeeb

Authors
  1. Younas Rasheed Tantray
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  2. Mohammad Saleem Wani
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Correspondence to Younas Rasheed Tantray.

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Tantray, Y.R., Wani, M.S., Pradhan, S.K. et al. Morphological, cytological and phytochemical studies in naturally occurring diploid and tetraploid populations of Physochlaina praealta from high altitudes of Trans-Himalaya. JPC-J Planar Chromat 33, 567–577 (2020). https://doi.org/10.1007/s00764-020-00075-4

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