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Salt Mediated Modulation of Autolysis of Thermolysin-Like Proteinase, Salilysin, Isolated from a Moderate Halophile, Chromohalobacter salexigens DSM3043

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Abstract

Halophilic salilysin is first synthesized as a pro-form, which has been shown autolysis activity to process pro-region (55 amino acids long) three times to form intermediate 1 (I1), intermediate 2 (I2) and final mature (M) salilysin. The autolysis of I1- to M-form salilysin in vitro was significantly accelerated with increasing NaCl concentration up to 4 M. Strong salting-out salts, (NH4)2SO4, Na2SO4 and MgSO4, were more effective, suggesting that autolysis is enhanced by inter-molecular association or structure compaction or both. However, MgCl2, a salting-in salt, was also effective, suggesting that other mechanisms, such as charge shielding and ionic binding to this halophilic protein, operated. Autolytic cleavage at site 3 resulted in mixed formation of correctly and incorrectly processed mature forms in the absence of salt, indicating that salt affected the accuracy of autolytic cleavage reaction. Far UV circular dichroism (CD) measurements indicated that E167A pro-salilysin showed an identical CD spectrum to the wild-type mature salilysin, suggesting pro-form has a proper fold for proteolytic activity. Thermal scanning indicated that E167A pro-salilysin was more heat-stable by ~ 10 °C than mature form. The CD spectra, thermal stability and modeling structure of salilysin clearly suggested that pro-salilysin is folded to the same structure as native form and is functional for autolysis.

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Data Availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

Abbreviations

I1:

Intermediate 1

I2:

Intermediate 2

M:

Mature

CD:

Circular dichroism

NTA:

Nitrilotriacetic acid

PAGE:

Polyacrylamide gel electrophoresis

NaPB:

Na-phosphate buffer

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Acknowledgements

We would like to thank M. Shimoyanagita, T. Yamashiro and N. Nagato for their study, discussion and helps.

Funding

This work was supported by Grant in Aid for Science Research (KAKENHI 20580372) from The Ministry of Education, Culture, Sports, Science and Technology (MEXT)Japan. This study was also supported by Sankei Science Foundation and by Institute for Fermentation, Osaka.

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Authors and Affiliations

  1. Applied and Molecular Microbiology, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima, 890-0065, Japan

    Matsujiro Ishibashi, Ryoichi Tanaka, Shunsuke Yamasaki, Hiroko Tokunaga & Masao Tokunaga

  2. Alliance Protein Laboratories, 13380 Pantera Rd, San Diego, CA, 92130, USA

    Tsutomu Arakawa

Authors
  1. Matsujiro Ishibashi
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  2. Ryoichi Tanaka
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  3. Shunsuke Yamasaki
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  4. Hiroko Tokunaga
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  5. Tsutomu Arakawa
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  6. Masao Tokunaga
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Contributions

MT corresponding author, conceptualization, investigation, and writing-original draft. MI investigation and formal analysis. RT and SY investigation. HT investigation and reviewing. TA writing—review & editing.

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Correspondence to Masao Tokunaga.

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Ishibashi, M., Tanaka, R., Yamasaki, S. et al. Salt Mediated Modulation of Autolysis of Thermolysin-Like Proteinase, Salilysin, Isolated from a Moderate Halophile, Chromohalobacter salexigens DSM3043. Protein J 40, 223–233 (2021). https://doi.org/10.1007/s10930-021-09964-x

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