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Development of Tunable Silk Fibroin/Xanthan Biopolymeric Scaffold for Skin Tissue Engineering Using L929 Fibroblast Cells

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Abstract

Skin acts as protective barrier against a number of factors such as dust, opportunistic microbial and viral infections, regulates body temperature and waste discharge. Fibroblast cell population plays an important role in development of skin architecture. A scaffold having capability to support and enhance fibroblast growth is a viable option for wound dressing material which can shorten the time for wound to heal. In this work, Silk Fibroin (SF) and Xanthan (Xa) were blended in three ratios 80 SF: 20 Xa (SFX82), 60 SF: 40 Xa (SFX64), and 50 SF: 50 Xa (SFX55) to create SF/Xa scaffold. Miscibility and other physicochemical properties of SF/Xa scaffold are functions of blending ratios and blend with the ratio 80 SF: 20 Xa has the highest miscibility. Thermal properties of SF/Xa blends are a function of miscibility with SFX82 having superior thermal properties of all fabricated scaffolds. The porosity of SF/Xa scaffolds is in the range of 67% to 50%, with pore size of 58.1 µm–45.5 µm, water uptake capacity of 92%–86%, and surface roughness of 49.95 nm–385 nm. SFX82 shows highest growth rate of L929 fibroblast cells indicating its superiority over other scaffolds for providing biological cues for the growth and proliferation of fibroblastic cells in natural environment. SFX82 scaffold is found to be most suitable for fibroblastic cells thereby enhancing the tissue regeneration at wound site.

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Acknowledgment

The first author thanks School of Biochemical Engineering, Indian Institute of Technology (Banaras Hindu University) and Ministry of Human Resource and Development, Government of India, for providing financial support regarding for carrying out the present research work. Authors also thank Central Instrument Facility Centre (CIFC), IIT (BHU) for providing characterization facilities and Sophisticated Analytical Instrument Facility (SAIF), Indian Institute of Technology, Bombay, for FTIR imaging and Thermal analysis facility.

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Authors and Affiliations

  1. Bioprocess Technology Laboratory, School of Biochemical Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi, 221005, India

    Shailendra Singh Shera & Rathindra Mohan Banik

  2. Department of Biotechnology, Faculty of Engineering & Technology, RAMA University Uttar Pradesh, Kanpur, 209217, India

    Shailendra Singh Shera

Authors
  1. Shailendra Singh Shera
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  2. Rathindra Mohan Banik
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Correspondence to Rathindra Mohan Banik.

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42235_2021_4_MOESM1_ESM.pdf

Development of Tunable Silk Fibroin/Xanthan Biopolymeric Scaffold for Skin Tissue Engineering Using L929 Fibroblast Cells

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Shera, S.S., Banik, R.M. Development of Tunable Silk Fibroin/Xanthan Biopolymeric Scaffold for Skin Tissue Engineering Using L929 Fibroblast Cells. J Bionic Eng 18, 103–117 (2021). https://doi.org/10.1007/s42235-021-0004-4

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  • DOI: https://doi.org/10.1007/s42235-021-0004-4

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