Phylogenetic diversity of indigenous Rhizobium trapped from the natural habitat of Pisum sativum L. in eastern and central Algeria

Gaci, Meriem; Benhizia, Yacine; Dems, Mohamed AbdEsselem; Boukaous, Leyla; Benguedouar, Ammar; Hynes, Michael Francis

doi:10.1007/s11104-020-04795-8

Phylogenetic diversity of indigenous Rhizobium trapped from the natural habitat of Pisum sativum L. in eastern and central Algeria

Regular Article
Published: 25 January 2021

Volume 461, pages 369–388, (2021)
Cite this article

Plant and Soil Aims and scope Submit manuscript

Meriem Gaci^1,2,
Yacine Benhizia¹,
Mohamed AbdEsselem Dems³,
Leyla Boukaous¹,
Ammar Benguedouar¹ &
…
Michael Francis Hynes ORCID: orcid.org/0000-0002-3608-2971²

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Abstract

Aims

The nitrogen-fixing symbiosis fixation between legumes and Rhizobium helps the plant to survive and to compete effectively on nitrogen poor soils. The soil environment attached to the root system is characterized by intense microbial activity. This work aimed to study the diversity of indigenous rhizobia living in the rhizosphere of forage pea in eastern and central Algeria.

Methods

Bacteria were trapped in vitro from collected soils. They were isolated from nodules by direct isolation or by bacterial trapping, and tested for nodulation. Further characterization was carried out to identify the isolates.

Results

Ninety-four isolates were obtained. Thirty-four were rhizobial strains that could, with two exceptions, establish root-nodule symbioses with the host plant. Different profiles of susceptibility to rhizobiophages indicated that the majority of isolates were likely to be members of the Rhizobium leguminosarum group of species. The strains revealed diverse plasmid profiles, and diverse restriction patterns of cpn60 gene amplicons and on Southern blot analysis of nodABC genes. Sequences of 16S rRNA gene showed that all isolates exhibited 99.85% identity with R. leguminosarum sv. viciae USDA 2370^T, R. laguerreae FB206^T and R. anhuiense CCBAU 23252^T. Analysis of cpn60 sequences revealed two groups. One of them clustered with ≤99% identity with R. laguerreae FB403. The second was clustered with ≤99% identity with R. laguerreae FB206^T.

Conclusion

Our findings suggest that the isolates may belong to the species R. laguerreae.

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Abbreviations

Rlv :: Rhizobium leguminosarum sv. viciae
LPS:: Lipopolysaccharide
bp:: bases pairs
ITGC:: Institut Technique des Grandes Cultures
EC:: Electrical Conductivity
OM:: Organic Matter
ANRH:: Agence Nationale des Ressources Hydrauliques
NRCS:: Natural Resources Conservation Service
USDA:: United States Department of Agriculture
subsp.:: subspecies
var.:: variety
cv.:: cultivar
TY:: Tryptone Yeast
SDW:: Shoot Dry Weight
YEM:: Yeast Extract-Mannitol
TAE:: Tris-acetate-EDTA
DIG:: digoxigenin
BLASTN:: Nucleotide Basic Local Alignment and Search Tool
cpnDB:: Chaperonin Sequence Database
MEGA:: Molecular Evolutionary Genetics Analysis
SD:: Standard Deviation
LSD:: Least Significant Difference
DCA:: Detrended correspondence analysis
RDA:: Redundancy Analysis
CR:: Congo Red

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Acknowledgements

This work was supported by a grant from the Ministry of Higher Education and Scientific Research of Algeria, and by a Natural Science and Engineering Research Council of Canada Discovery grant to MFH.

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Authors and Affiliations

Laboratoire de Biologie Moléculaire et Cellulaire, Département de Microbiologie, Faculté des Sciences de la Nature et de la Vie, Université Frères Mentouri Constantine 1, Route de Aïn El Bey, Constantine, Algeria
Meriem Gaci, Yacine Benhizia, Leyla Boukaous & Ammar Benguedouar
Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta, T2N 1N4, Canada
Meriem Gaci & Michael Francis Hynes
Centre de Recherche en Biotechnologie, Ali Mendjli Nouvelle Ville UV 03, BP E73, Constantine, Algeria
Mohamed AbdEsselem Dems

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Meriem Gaci
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Yacine Benhizia
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Mohamed AbdEsselem Dems
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Leyla Boukaous
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Ammar Benguedouar
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Michael Francis Hynes
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Correspondence to Michael Francis Hynes.

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Gaci, M., Benhizia, Y., Dems, M.A. et al. Phylogenetic diversity of indigenous Rhizobium trapped from the natural habitat of Pisum sativum L. in eastern and central Algeria. Plant Soil 461, 369–388 (2021). https://doi.org/10.1007/s11104-020-04795-8

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Received: 17 July 2020
Accepted: 03 December 2020
Published: 25 January 2021
Issue Date: April 2021
DOI: https://doi.org/10.1007/s11104-020-04795-8

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Phylogenetic diversity of indigenous Rhizobium trapped from the natural habitat of Pisum sativum L. in eastern and central Algeria

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