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The glomerular network of the zebrafish olfactory bulb

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Abstract

Each zebrafish olfactory bulb contains ~ 140 glomeruli that are distinguishable based on size, location, neurochemistry and function. Here we examine the mitral cell innervation of differently sized glomeruli in adult zebrafish. Type 1 glomeruli had diameters of 80.9 ± 8.1 μm and were innervated by 5.9 ± 0.9 mitral cells. The Type 1 mediodorsal glomeruli (mdG) were innervated by both uniglomerular (innervating only single glomeruli) and multiglomerular mitral cells (innervating two or more glomeruli). In contrast, the Type 1 ventroposterior (vpG) and lateral glomeruli (lG) were only innervated by uniglomerular mitral cells. Type 2 ventral glomeruli were 46 ± 5.1 μm in diameter and were innervated by 3.3 ± 0.2 mitral cells. Type 2 ventromedial glomeruli (vmG) were innervated exclusively by uniglomerular mitral cells. Type 3 glomeruli had diameters of 17 ± 2.5 μm and were innervated by 1.1 ± 0.6 multiglomerular mitral cells each. Finally, Type 4 glomeruli were small, with average diameters of 4.8 ± 3.9 μm and were restricted to the lateral plexus. These glomeruli were innervated mainly by multiglomerular mitral cells with extensively branching dendrites. This study provides the first specific associations between uni- and multiglomerular mitral cells with known zebrafish glomeruli. Our results suggest that glomeruli are distinguishable based on their postsynaptic compartment and that distinct input–output computations occur in different types of zebrafish glomeruli.

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Abbreviations

dG:

Dorsal glomerulus

dlG:

Dorsolateral glomerulus

lG:

Lateral glomerulus

lP:

Lateral plexus

maG:

Medial anterior glomerulus

mdG:

Mediodorsal glomerulus

mpG:

Medial posterior glomerulus

vaG:

Ventroanterior glomerulus

vmG:

Ventromedial glomerulus

vpG:

Ventroposterior glomerulus

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Acknowledgements

This work was funded by NSERC (Canada Discovery Grant RGPIN-2018–04593) and CIHR (Canada Open Operating Grant INMHA 330,849) and fueled by the Tim Hortons Walnut Crunch. The authors thank Song Ea Beak for her help with the figure preparation.

Funding

This work was funded by NSERC (Canada Discovery Grant RGPIN-2018–04593) and CIHR (Canada Open Operating Grant INMHA 330849).

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Authors and Affiliations

  1. Department of Physiology and Biophysics, Dalhousie University, Halifax, Nova Scotia, B3H4R2, Canada

    Oliver Braubach & Roger P. Croll

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  1. Oliver Braubach
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  2. Roger P. Croll
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Contributions

OB conceived the experiments; OB conducted the experiments and interpreted the data. OB and RC wrote the manuscript.

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Correspondence to Oliver Braubach.

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The authors declare that there is no conflict of interest regarding the publication of this article.

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Experiments adhered to guidelines established by the Canadian animal Council for Animal Care and were approved by the committee for animal care at Dalhousie University.

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Braubach, O., Croll, R.P. The glomerular network of the zebrafish olfactory bulb. Cell Tissue Res 383, 255–271 (2021). https://doi.org/10.1007/s00441-020-03394-4

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