Issue 8, 2021, Issue in Progress

Quantifying genome DNA during whole-genome amplification via quantitative real-time multiple displacement amplification

Abstract

DNA quantification is important in the research of life sciences. In an independent quantification process, the extracted part of a DNA sample is usually difficult to be recycled for further use while the widely used real-time PCR is used to count the copies with certain sequences. Based on the popular multiple displacement amplification (MDA), we proposed and performed quantitative real-time MDA to obtain the information of template amount based on fluorescence signals while amplifying whole-genome DNA. The detection limit of real-time MDA was as low as 0.5 pg μl−1 (5 pg DNA input), offering the whole-genome research a promising tool to quantify the entire DNA during amplification without sacrificing sample completeness or introducing redundant steps.

Graphical abstract: Quantifying genome DNA during whole-genome amplification via quantitative real-time multiple displacement amplification

Supplementary files

Article information

Article type
Paper
Submitted
23 Oct 2020
Accepted
28 Dec 2020
First published
22 Jan 2021
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2021,11, 4617-4621

Quantifying genome DNA during whole-genome amplification via quantitative real-time multiple displacement amplification

J. Tu, Y. Qiao, Y. Luo, N. Long and Z. Lu, RSC Adv., 2021, 11, 4617 DOI: 10.1039/D0RA09021B

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