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Isoptericola sediminis sp. nov., Isolated from Chilika Lagoon

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Abstract

A yellow-colored, Gram-stain-positive, rod shaped, non-motile bacterium, designated as strain JC619T, was isolated from the sediment of Chilika lagoon, India. Strain JC619T shows highest 16S rRNA gene sequence similarity (99.08%) with Isoptericola chiayiensis KCTC 19740T followed by Isoptericola halotolerans KCTC 19046T (98.6%) and other members of the genus Isoptericola (< 98%). NaCl is required for growth of strain JC619T and tolerates up to 18% (w/v) and pH up to 10. Strain JC619T grows optimally at temperature 30 °C, NaCl concentration of 3% (w/v), and at pH 7.5. The genome size of strain JC619T is 3.2 Mb with G+C content of 73.0 mol%. ANI scores of strain JC619T are 81.9% and 80.1% and dDDH values are 24.4% and 22.7% with I. chiayiensis KCTC 19740T and I. halotolerans KCTC 19046T, respectively. Respiratory quinones are MK-9(H4) and MK-9(H2). Predominant fatty acids (> 10%) are anteiso-C15:0, iso-C16:0, and iso-C15:0. Major polar lipids of strain JC619T are phosphatidylglycerol, phosphatidylinositolmannoside, diphosphatidylglycerol, and phosphatidylinositol. Strain JC619T is catalase positive but cytochrome oxidase negative and reduces nitrate. The genomic distinction of strain JC619T with its nearest related species of the genus Isoptericola is well supported with chemotaxonomic characteristics and differential physiological properties. Therefore, strain JC619T represents a new species under the genus Isoptericola for which Isoptericola sediminis sp. nov. is proposed. Type strain is JC619T (=KCTC 49244T =NBRC 114063T).

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Authors and Affiliations

  1. University of Hyderabad School of Life Sciences, Hyderabad, Telangana, India

    Dhanesh Kumar, A. Krishnaiah, G. Suresh & Ch. Venkata Ramana

  2. Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, J. N. T. University Hyderabad, Kukatpally, Hyderabad, 500085, India

    U. Jagadeeshwari & Ch. Sasikala

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  1. Dhanesh Kumar
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  2. U. Jagadeeshwari
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  3. A. Krishnaiah
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  4. G. Suresh
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  5. Ch. Sasikala
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  6. Ch. Venkata Ramana
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Correspondence to Ch. Sasikala or Ch. Venkata Ramana.

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Kumar, D., U., J., A., K. et al. Isoptericola sediminis sp. nov., Isolated from Chilika Lagoon. Curr Microbiol 78, 848–855 (2021). https://doi.org/10.1007/s00284-020-02325-4

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