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Formulation development of cream with mupirocin and essential oils for eradication of biofilm mediated antimicrobial resistance

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Abstract

Staphylococcus aureus (S.aureus) is both a colonizer as well as a human pathogen that causes a variety of diseases. Mupirocin is a topical antimicrobial agent which is very effective against S.aureus infection. However, treating the S.aureus infection using mupirocin could be complicated due to biofilm formation. Consequently, resistance to mupirocin occurs and leads to chronic infection. The combination of mupirocin with a compound that has biofilm eradicating effect would be an ideal solution for effectively treating biofilm infections. Therefore, in this study, we have investigated the biofilm inhibitory and eradication effect of mupirocin with three essential oils (Cinnamon Oil (CO), Eugenol (EU) and Eucalyptus Oil (EO)) against sessile S.aureus. From these preliminary results, it was found that the mupirocin-CO (0.2 µg/ml-5.218 mg/ml) combination has a better synergistic antibiofilm effect against sessile S.aureus and the fractional inhibitory concentration index was found to be 0.458. The best combination of mupirocin with CO was loaded into a non-greasy O/W cream. The physico-chemical and microbiological evaluations were carried out for the prepared cream. The prepared cream has better biofilm eradication activity (40%) when compared to a marketed cream (20%).

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Acknowledgements

We would like to thank the Principal, PSG College of Pharmacy and PSG Insitute of Medical Sciences and Research for providing necessary facilities to do the work. We also thank Fourts India, Stedman Pharmaceuticals Pvt Ltd Chennai for providing free gift sample of Mupriocin and Council of Scientific and Industrial Research, New Delhi for providing a major extramural research grant to initiate the preliminary research work (Grant. No: 60(0117)/19/EMR-II). We also acknowledge The Tamil Nadu Dr.M.G.R. Medical University, Chennai, India for providing minor research grant and Dr Sivaram Hariharan for English proofreading and editing.

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Authors and Affiliations

  1. Department of Pharmaceutics, PSG College of Pharmacy, Coimbatore, India, 641 004

    Mahalakshmi Sundaramoorthy, Arjunan Karuppaiah, Munusamy Nithyanth, Rajandurai Baberoselin & Sankar Veintramuthu

  2. Affiliated to The Tamilnadu Dr.M.G.R Medical University, Chennai, India

    Mahalakshmi Sundaramoorthy, Arjunan Karuppaiah, Munusamy Nithyanth, Rajandurai Baberoselin & Sankar Veintramuthu

  3. Department of Pharmaceutics, Ratnam Institute of Pharmacy, Nellore, India, 524 346

    Santhanam Ramesh

  4. Department of Botany, Bharathiar University, Coimbatore, India, 641046

    Natesan Geetha

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  1. Mahalakshmi Sundaramoorthy
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  2. Arjunan Karuppaiah
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  3. Munusamy Nithyanth
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  4. Rajandurai Baberoselin
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  5. Santhanam Ramesh
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  6. Natesan Geetha
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  7. Sankar Veintramuthu
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Correspondence to Sankar Veintramuthu.

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Communicated by Erko Stackebrandt.

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Sundaramoorthy, M., Karuppaiah, A., Nithyanth, M. et al. Formulation development of cream with mupirocin and essential oils for eradication of biofilm mediated antimicrobial resistance. Arch Microbiol 203, 1707–1715 (2021). https://doi.org/10.1007/s00203-020-02175-5

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  • DOI: https://doi.org/10.1007/s00203-020-02175-5

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