We studied respiratory activity and microviscosity of mitochondrial membranes from the left ventricular myocardium of 2-month-old (n=8) and 15-month-old (n=8) rats. The respiratory control (RC) during substrate phosphorylation was calculated as the ratio of oxygen absorption rates in the presence of ADP and after its utilization. In 2-month-old rats, RC was 4.66 (4.56; 4.71); in 15-month-old animals, it was significantly (р<0.05) lower: 3.57 (3.50; 3.62). Pyrene probe eximerization indices for regions of protein-lipid and lipid-lipid interactions in mitochondria of 2-month-old animals were significantly lower than in the group of 15-month-old rats, which indicated reduced microviscosity of the lipid environment of proteins. Thus, the decrease in RC of mitochondria from the left ventricle of 15-month-old animals and the increase in the microviscosity coefficients of their membranes indicate age-related changes in the structural and functional activity of mitochondria.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 170, No. 9, pp. 366-369, September, 2020
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Rebrova, T.Y., Korepanov, V.A. & Afanasiev, S.A. Age Peculiarities of Respiratory Activity and Membrane Microviscosity of Mitochondria from Rat Cardiomyocytes. Bull Exp Biol Med 170, 368–370 (2021). https://doi.org/10.1007/s10517-021-05069-8
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DOI: https://doi.org/10.1007/s10517-021-05069-8